Effects of size and dimension in optic relaxation in Rb vapor

We report on an experiment in which the fluorescence decay time of 5P levels of Rb atoms in a coated vapor cell exceeds several of milliseconds that many orders of magnitude longer than normal decay time of excited of rubidium atoms. This effect has been observed by using the pump laser with fixing frequency and the periodically scan the probing laser frequency of which overlap all Rb hyperfine linens of 5P-5S transition. Long time fluorescence decay take place in wide part of the spherical high quality coated cell. In cylindrical part of the cell or in uncoated cell the long time fluorescence decay was disappeared.

Evaporative deposition of SiO2 nanoparticles multilayer on ITO substrates and application for vertical alignment of liquid crystals - Effect of dichroic dye

In this work the SiO2 nanoparticles layer was deposited by solvent evaporation on ITO substrates and used for vertical alignment of pure and dye doped liquid crystal (LC) in confined cells. The pure nematic liquid crystal (NLC) material was filled into SiO2 layer coated cell by the capillary injection method. The 0.5 wt.% of dichroic dye added into the LC mixture and studied its effect on electro-optic behaviour of display cell. The FESEM study reveals the multilayered deposition of the SiO2 on Indium tin oxide (ITO) substrates. Conoscopic and polarised optical microscopy (POM) study show good dark black and bright textures at OFF and ON state under the cross polarizers and confirm vertical alignment (VA) of LC molecules. The electro-optical (E-O) study shows the lower threshold and operating voltage with higher contrast ratio in dye doped vertically aligned LC (VALC) cell as compare to the pure SiO2 coated VALC cell.

STABILITY ENHANCEMENT OF ALUMINUM-AIR BATTERY

A comparative analysis is presented for an aluminum-air battery with a carbon-coated and non-coated anode made of 4N pure aluminum with the purpose to enhance the stability of the battery. The carbon coating was proven to be quite effective which lasted almost two times more than the non-coated cell with little to almost no effect on the electrochemical behavior. A method was also proposed to limit the self-discharge electrode corrosion of the aluminum-air battery by limiting the oxygen supply to the cell from atmospheric air. The blockage of the air supply limits the oxidation-reduction reaction necessary for cell operation. For that purpose, the cell was tested in vacuum condition for 25 days which showed quite impressive results when compared with the cell kept in a non-vacuum room condition. It had retained its potential as well as resisted the corrosion quite well with almost negligible weight loss and byproduct accumulation

Design of RGDS Peptide-Immobilized Self-Assembling β-Strand Peptide from Barnacle Protein

We designed three types of RGD-containing barnacle adhesive proteins using self-assembling peptides. In the present study, three types of RGD-containing peptides were synthesized by solid-phase peptide synthesis, and the secondary structures of these peptides were analyzed by CD and FT-IR spectroscopy. The mechanical properties of peptide hydrogels were characterized by a rheometer. We discuss the correlation between the peptide conformation, and cell attachment and cell spreading activity from the viewpoint of developing effective tissue engineering scaffolds. We created a peptide-coated cell culture substrate by coating peptides on a polystyrene plate. They significantly facilitated cell adhesion and spreading compared to a non-coated substrate. When the RGDS sequence was modified at N- or C-terminal of R-Y, it was found that the self-assembling ability was dependent on the strongly affects hydrogel formation and cell adhesion caused by its secondary structure.

Development of pH-Responsive Polymer Coating as an Alternative to Enzyme-Based Stem Cell Dissociation for Cell Therapy

Cell therapy usually accompanies cell detachment as an essential process in cell culture and cell collection for transplantation. However, conventional methods based on enzymatic cell detachment can cause cellular damage including cell death and senescence during the routine cell detaching step due to an inappropriate handing. The aim of the current study is to apply the pH-responsive degradation property of poly (amino ester) to the surface of a cell culture dish to provide a simple and easy alternative method for cell detachment that can substitute the conventional enzyme treatment. In this study, poly (amino ester) was modified (cell detachable polymer, CDP) to show appropriate pH-responsive degradation under mild acidic conditions (0.05% (w/v) CDP, pH 6.0) to detach stem cells (human adipose tissue-derived stem cells (hADSCs)) perfectly within a short period (less than 10 min). Compared to conventional enzymatic cell detachment, hADSCs cultured on and detached from a CDP-coated cell culture dish showed similar cellular properties. We further performed in vivo experiments on a mouse hindlimb ischemia model (1.0 × 106 cells per limb). The in vivo results indicated that hADSCs retrieved from normal cell culture dishes and CDP-coated cell culture dishes showed analogous therapeutic angiogenesis. In conclusion, CDP could be applied to a pH-responsive cell detachment system as a simple and easy nonenzymatic method for stem cell culture and various cell therapies.

Au–Fe3O4 nanoagent coated cell membrane for targeted delivery and enhanced chem/photo therapy

We report the preparation of a Au–Fe3O4 nanoagent cell membrane coating, and the treatment process in cell.