Design and analysis of tissue engineering scaffolds that mimic soft tissue mechanical anisotropy

Additive manufacturing, or three-dimensional (3D) printing, has emerged as a viable technique for the production of vascularized tissue engineering scaffolds. In this report, a biocompatible and biodegradable poly(tri(ethylene glycol) adipate) dimethacrylate was synthesized and characterized for suitability in soft-tissue scaffolding applications. The polyester dimethacrylate exhibited highly efficient photocuring, hydrolyzability, and 3D printability in a custom microstereolithography system. The photocured polyester film demonstrated significantly improved cell attachment and viability as compared with controls. These results indicate promise of novel, printable polyesters for 3D patterned, vascularized soft-tissue engineering scaffolds.

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4D polycarbonates via stereolithography as scaffolds for soft tissue repair

Nature Communications ◽

10.1038/s41467-021-23956-6 ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Andrew C. Weems ◽

Maria C. Arno ◽

Wei Yu ◽

Robert T. R. Huckstepp ◽

Andrew P. Dove

Keyword(s):

Tissue Engineering ◽

Soft Tissue ◽

Healing Process ◽

Surface Erosion ◽

Tissue Engineering Scaffolds ◽

Adipose Tissue Engineering ◽

Filling Materials ◽

Aliphatic Polycarbonate ◽

Soft Tissue Engineering

Abstract3D printing has emerged as one of the most promising tools to overcome the processing and morphological limitations of traditional tissue engineering scaffold design. However, there is a need for improved minimally invasive, void-filling materials to provide mechanical support, biocompatibility, and surface erosion characteristics to ensure consistent tissue support during the healing process. Herein, soft, elastomeric aliphatic polycarbonate-based materials were designed to undergo photopolymerization into supportive soft tissue engineering scaffolds. The 4D nature of the printed scaffolds is manifested in their shape memory properties, which allows them to fill model soft tissue voids without deforming the surrounding material. In vivo, adipocyte lobules were found to infiltrate the surface-eroding scaffold within 2 months, and neovascularization was observed over the same time. Notably, reduced collagen capsule thickness indicates that these scaffolds are highly promising for adipose tissue engineering and repair.

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Apigenin Induces Browning in White Adipocytes Mediated by VEGF-PRDM16 Signaling.

10.21203/rs.3.rs-1210533/v1 ◽

2022 ◽

Author(s):

Sreelekshmi Sreeku ◽

Vinu Vijayan ◽

Fathe Singh ◽

Manu Sudhakar ◽

Kiran M S

Keyword(s):

Tissue Engineering ◽

Soft Tissue ◽

De Novo ◽

Metabolic Shift ◽

Tissue Engineering Scaffolds ◽

White Adipocyte ◽

White Adipocytes ◽

Soft Tissue Engineering ◽

Treatment Of Obesity ◽

Endothelial Growth Factor

Abstract The white adipose tissues are metabolically inert which results in deranged biological signalling disorders resulting in obesity. Lack of vascularisation in these tissues is mainly responsible to make them metabolically inert. Not much work has been done in this direction to understand the role of angiogenesis in white adipocytes metabolism. In the present study, we evaluated the effect of angiogenic modulator in modulating the metabolism in white adipocyte. Nutraceuticals apigenin (Apg) was employed as angiogenic modulator. The results indicated that promoting angiogenesis by Apg enhanced the de novo differentiation and trans-differentiation of white adipocyte into brown like phenotype by triggering vascular endothelial growth factor A. Cross talk between endothelial and adipocytes were observed in co-culture studies. The metabolic shift in white adipocytes was observed to be due to the upregulation of PRDM16 cascade. The study provides new insights for inducing metabolic shift in white adipocytes by modulation of angiogenesis in white adipocyte to trigger browning for the treatment of obesity. Further the study opens scopes for development of medical devices for obese subjects, an area that needs to be addressed specifically with reference to soft tissue engineering as commercial soft tissue engineering scaffolds does not suit the obese patients.

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Novel tissue engineering scaffolds as wound dressings loaded with Alkannins/Shikonins as active ingredients

10.1055/s-0039-3400068 ◽

2019 ◽

Author(s):

AS Arampatzis ◽

K Theodoridis ◽

E Aggelidou ◽

KN Kontogiannopoulos ◽

I Tsivintzelis ◽

...

Keyword(s):

Tissue Engineering ◽

Wound Dressings ◽

Active Ingredients ◽

Tissue Engineering Scaffolds

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HYDROXYAPATITE, ALGINATE, AND CHITOSAN FOR BONE SCAFFOLDS: SPECTROSCOPY STUDY

Dentika Dental Journal ◽

10.32734/dentika.v19i2.457 ◽

2016 ◽

Vol 19 (2) ◽

pp. 93-100

Author(s):

Lalita El Milla

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Functional Groups ◽

Bone Growth ◽

Three Dimensional ◽

Dimensional Structure ◽

Tissue Engineering Scaffolds ◽

Hydroxyapatite Powder ◽

Materials Used

Scaffolds is three dimensional structure that serves as a framework for bone growth. Natural materials are often used in synthesis of bone tissue engineering scaffolds with respect to compliance with the content of the human body. Among the materials used to make scafffold was hydroxyapatite, alginate and chitosan. Hydroxyapatite powder obtained by mixing phosphoric acid and calcium hydroxide, alginate powders extracted from brown algae and chitosan powder acetylated from crab. The purpose of this study was to examine the functional groups of hydroxyapatite, alginate and chitosan. The method used in this study was laboratory experimental using Fourier Transform Infrared (FTIR) spectroscopy for hydroxyapatite, alginate and chitosan powders. The results indicated the presence of functional groups PO43-, O-H and CO32- in hydroxyapatite. In alginate there were O-H, C=O, COOH and C-O-C functional groups, whereas in chitosan there were O-H, N-H, C=O, C-N, and C-O-C. It was concluded that the third material containing functional groups as found in humans that correspond to the scaffolds material in bone tissue engineering.

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