Hyaluronic acid hydrogels with controlled degradation properties for oriented bone regeneration

AbstractHyaluronic acid (HA) is widely distributed in the human body, and it is heavily involved in many physiological functions such as tissue hydration, wound repair, and cell migration. In recent years, HA and its derivatives have been widely used as advanced bioactive polymers for bone regeneration. Many medical products containing HA have been developed because this natural polymer has been proven to be nontoxic, noninflammatory, biodegradable, and biocompatible. Moreover, HA-based composite scaffolds have shown good potential for promoting osteogenesis and mineralization. Recently, many HA-based biomaterials have been fabricated for bone regeneration by combining with electrospinning and 3D printing technology. In this review, the polymer structures, processing, properties, and applications in bone tissue engineering are summarized. The challenges and prospects of HA polymers are also discussed.

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Photocuring Hyaluronic Acid/Silk Fibroin Hydrogel Containing Curcumin Loaded CHITOSAN Nanoparticles for the Treatment of MG-63 Cells and ME3T3-E1 Cells

Polymers ◽

10.3390/polym13142302 ◽

2021 ◽

Vol 13 (14) ◽

pp. 2302

Author(s):

Qingwen Yu ◽

Zhiyuan Meng ◽

Yichao Liu ◽

Zehao Li ◽

Xing Sun ◽

...

Keyword(s):

Hyaluronic Acid ◽

Bone Regeneration ◽

Water Uptake ◽

Silk Fibroin ◽

Bone Repair ◽

Ph Value ◽

Chitosan Nanoparticles ◽

Vitro Assay ◽

Long Term Treatment

After an osteosarcoma excision, recurrence and bone defects are significant challenges for clinicians. In this study, the curcumin (Cur) loaded chitosan (CS) nanoparticles (CCNP) encapsulated silk fibroin (SF)/hyaluronic acid esterified by methacrylate (HAMA) (CCNPs-SF/HAMA) hydrogel for the osteosarcoma therapy and bone regeneration was developed by photocuring and ethanol treatment. The micro or nanofibers networks were observed in the CCNPs-SF/HAMA hydrogel. The FTIR results demonstrated that alcohol vapor treatment caused an increase in β-sheets of SF, resulting in the high compression stress and Young’s modulus of CCNPs-SF/HAMA hydrogel. According to the water uptake analysis, SF caused a slight decrease in water uptake of CCNPs-SF/HAMA hydrogel while CCNPs could enhance the water uptake of it. The swelling kinetic results showed that both the CCNPs and the SF increased the swelling ratio of CCNPs-SF/HAMA hydrogel. The accumulative release profile of CCNPs-SF/HAMA hydrogel showed that the release of Cur from CCNPs-SF/HAMA hydrogel was accelerated when pH value was decreased from 7.4 to 5.5. Besides, compared with CCNPs, the CCNPs-SF/HAMA hydrogel had a more sustainable drug release, which was beneficial for the long-term treatment of osteosarcoma. In vitro assay results indicated that CCNPs-SF/HAMA hydrogel with equivalent Cur concentration of 150 μg/mL possessed both the effect of anti-cancer and promoting the proliferation of osteoblasts. These results suggest that CCNPs-SF/HAMA hydrogel with superior physical properties and the bifunctional osteosarcoma therapy and bone repair may be an excellent candidate for local cancer therapy and bone regeneration.

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Development of an Injectable Calcium Phosphate/Hyaluronic Acid Microparticles System for Platelet Lysate Sustained Delivery Aiming Bone Regeneration

Macromolecular Bioscience ◽

10.1002/mabi.201600141 ◽

2016 ◽

Vol 16 (11) ◽

pp. 1662-1677 ◽

Cited By ~ 15

Author(s):

Pedro S. Babo ◽

Vítor E. Santo ◽

Manuela E. Gomes ◽

Rui L. Reis

Keyword(s):

Hyaluronic Acid ◽

Calcium Phosphate ◽

Bone Regeneration ◽

Platelet Lysate ◽

Sustained Delivery

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COMBINED USE OF HYALURONIC ACID WITH NANO-BIOACTIVE GLASS ENHANCED BIOCEMENT BASED SILICATE STIMULATED BONE REGENERATIVE CAPACITY IN TIBIAL BONE DEFECTS OF RABBITS: IN-VIVO STUDY

Journal of Experimental Biology and Agricultural Sciences ◽

10.18006/2021.9(5).630.638 ◽

2021 ◽

Vol 9 (5) ◽

pp. 630-638

Author(s):

Fatema Aziz Al-Sayed ◽

◽

Radwa Hamed Hegazy ◽

Zeinab Amin Salem ◽

Hanan Hassan El-Beheiry ◽

...

Keyword(s):

Hyaluronic Acid ◽

Bioactive Glass ◽

Bone Regeneration ◽

Bone Defects ◽

Bone Area ◽

Regenerative Capacity ◽

Tibial Bone ◽

Group 3 ◽

Area Percentage ◽

Group 1

An ideal biomaterial for bone regeneration is a longstanding quest nowadays. This study aimed to evaluate the osteogenic potentiality of nano-bioactive glass enhanced biocement based silicate with or without hyaluronic acid seeded in rabbits’ tibial bone defects. For this, 24 male rabbits with two 5 mm defects (1 defect per tibia) were divided into three equal groups. Among the predefined three groups, for the rabbits of group 1(control) bone defects were left untreated while for the members of group 2 defects received nano-bioactive glass enhanced biocement based silicate cement, and group 3 defects received nano-bioactive glass cement mixed with hyaluronic acid. Animals of each group were divided equally for euthanization after 3 and 6 weeks. Bone specimens were processed and examined histologically with histomorphometrically analysis of new bone area percentage. The bone defects in group 3 showed significantly improved osseous healing histologically as compared to the group 1&2. The morphometric analysis also revealed a significant increase in the new bone area percentage in group 3 as compared to the group 1 and 2 (P < 0.05). The results of the present study can be concluded that bone defects could be treated with nano-bioactive glass and hyaluronic acid cement. Although, nano-bioactive glass alone was capable of bone regeneration the combination of both had significant regenerative capacity.

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