Cellular uptake pathways of lipid-modified cationic polymers in gene delivery to primary cells

In the past few years gene delivery system has gained a huge attention owing to its proved efficacy in several diseases especially in those caused by genetic and/oroncological malfunctioning. The effective gene delivery mainly depends on the carrier molecules that can ensure the safe and specific delivery of the nucleic acidmolecules. Viral vectors have been used for a longer period as the gene transfer vehicle. However, these viral vectors have potential immunological disadvantages that made them less preferred. Recently, non-viral vectors such as polyplexes have emerged as a promising alternative for viral vectors. Polyplexes are formed by conjugating a polymer with DNA and in maximum cases the cationic polymers are preferred over others. The structure and stability of the polyplexes depends on various factors. The ability of the polymer to condense the DNA mainly dictates the efficiency of the polyplex mediated transfection. In this review we are going to provide a framework for the synthesis and design of the polyplexes along with the structure and stability of the complexes pertaining to mechanism of action, characterization and therapeutic application, including polyethyleneimine mediated cytotoxicity as well as newer strategies for the generation of better polyplexes.

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Brushlike Cationic Polymers with Low Charge Density for Gene Delivery

Biomacromolecules ◽

10.1021/acs.biomac.7b01267 ◽

2017 ◽

Vol 19 (5) ◽

pp. 1410-1415 ◽

Cited By ~ 8

Author(s):

Jonathan O’Keeffe Ahern ◽

Sigen A ◽

Dezhong Zhou ◽

Yongsheng Gao ◽

Jing Lyu ◽

...

Keyword(s):

Gene Delivery ◽

Charge Density ◽

Cationic Polymers ◽

Low Charge

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Cellular uptake and gene delivery using layered double hydroxide nanoparticles

Journal of Materials Chemistry B ◽

10.1039/c2tb00081d ◽

2013 ◽

Vol 1 (1) ◽

pp. 61-68 ◽

Cited By ~ 63

Author(s):

Shuangde Li ◽

Jinghuan Li ◽

Chengle J. Wang ◽

Qiang Wang ◽

M. Zameel Cader ◽

...

Keyword(s):

Gene Delivery ◽

Layered Double Hydroxide ◽

Cellular Uptake ◽

Double Hydroxide ◽

Layered Double Hydroxide Nanoparticles

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Nonviral Vehicles for Gene Delivery

Nano LIFE ◽

10.1142/s1793984421300028 ◽

2021 ◽

Vol 11 (02) ◽

pp. 2130002

Author(s):

Eric Warga ◽

Brian Austin-Carter ◽

Noelle Comolli ◽

Jacob Elmer

Keyword(s):

Gene Delivery ◽

Lower Cost ◽

Clinical Applications ◽

Inorganic Nanoparticles ◽

Nonviral Gene Delivery ◽

Viral Gene ◽

Cationic Polymers ◽

Recent Developments ◽

Low Efficiency ◽

Viral Gene Delivery

Nonviral gene delivery (NVGD) is an appealing alternative to viral gene delivery for clinical applications due to its lower cost and increased safety. A variety of promising nonviral vectors are under development, including cationic polymers, lipids, lipid-polymer hybrids (LPHs) and inorganic nanoparticles. However, some NVGD strategies have disadvantages that have limited their adoption, including high toxicity and low efficiency. This review focuses on the most common NVGD vehicles with an emphasis on recent developments in the field.

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Multicomponent Polymerization toward Cationic Polymers for Efficient Gene Delivery

Macromolecular Rapid Communications ◽

10.1002/marc.202000464 ◽

2020 ◽

pp. 2000464

Author(s):

Nan Zheng ◽

Daniel Kwesi Cudjoe ◽

Wangze Song

Keyword(s):

Gene Delivery ◽

Cationic Polymers ◽

Efficient Gene

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Dendron conjugation to graphene oxide using click chemistry for efficient gene delivery

RSC Advances ◽

10.1039/c5ra07004j ◽

2015 ◽

Vol 5 (62) ◽

pp. 50196-50211 ◽

Cited By ~ 21

Author(s):

Kishor Sarkar ◽

Giridhar Madras ◽

Kaushik Chatterjee

Keyword(s):

Graphene Oxide ◽

Gene Delivery ◽

Click Chemistry ◽

Surface Area ◽

Cellular Uptake ◽

Large Surface Area ◽

Efficient Gene ◽

Attractive Candidate

Owing to its large surface area and rapid cellular uptake, graphene oxide (GO) is emerging as an attractive candidate material for delivery of drugs and genes.

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