3D cell printing of in vitro stabilized skin model and in vivo pre-vascularized skin patch using tissue-specific extracellular matrix bioink: A step towards advanced skin tissue engineering

Reconstruction of skin defects is often a challenging effort due to the currently limited reconstructive options. In this sense, tissue engineering has emerged as a possible alternative to replace or repair diseased or damaged tissues from the patient’s own cells. A substantial number of tissue-engineered skin substitutes (TESSs) have been conceived and evaluated in vitro and in vivo showing promising results in the preclinical stage. However, only a few constructs have been used in the clinic. The lack of standardization in evaluation methods employed may in part be responsible for this discrepancy. This review covers the most well-known and up-to-date methods for evaluating the optimization of new TESSs and orientative guidelines for the evaluation of TESSs are proposed.

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Needleless electrospinning of PVP/PGS fibrous scaffolds for skin tissue engineering applications

10.31224/osf.io/xs64k ◽

2018 ◽

Cited By ~ 1

Author(s):

Antonios Keirouz ◽

Giuseppino Fortunato ◽

Anthony Callanan ◽

Norbert Radacsi

Keyword(s):

Tissue Engineering ◽

Tensile Modulus ◽

Dermal Fibroblasts ◽

Skin Tissue ◽

Skin Substitute ◽

Electrospinning Technique ◽

Skin Tissue Engineering ◽

Needleless Electrospinning ◽

High Concentrations

Scaffolds and implants used for tissue engineering need to be adapted for their mechanical properties with respect to their environment within the human body. Therefore, a novel composite for skin tissue engineering is presented by use of blends of Poly(vinylpyrrolidone) (PVP) and Poly(glycerol sebacate) (PGS) were fabricated via the needleless electrospinning technique. The formed PGS/PVP blends were morphologically, thermochemically and mechanically characterized. The morphology of the developed fibers related to the concentration of PGS, with high concentrations of PGS merging the fibers together plasticizing the scaffold. The tensile modulus appeared to be affected by the concentration of PGS within the blends, with an apparent decrease in the elastic modulus of the electrospun mats and an exponential increase of the elongation at break. Ultraviolet (UV) crosslinking of PGS/PVP significantly decreased and stabilized the wettability of the formed fiber mats, as indicated by contact angle measurements. In vitro examination showed good viability and proliferation of human dermal fibroblasts over the period of a week. The present findings provide important insights for tuning the elastic properties of electrospun material by incorporating this unique elastomer, as a promising future candidate for skin substitute constructs.

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An in vitro evaluation of Genipin‐crosslinked and Hypericum perforatum incorporated novel membranes for skin tissue engineering applications

Journal of Applied Polymer Science ◽

10.1002/app.51385 ◽

2021 ◽

pp. 51385

Author(s):

Seda Ceylan

Keyword(s):

Tissue Engineering ◽

Hypericum Perforatum ◽

Skin Tissue ◽

Engineering Applications ◽

In Vitro Evaluation ◽

Skin Tissue Engineering

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Recapitulating Cardiac Structure and Function In Vitro from Simple to Complex Engineering

Micromachines ◽

10.3390/mi12040386 ◽

2021 ◽

Vol 12 (4) ◽

pp. 386

Author(s):

Ana Santos ◽

Yongjun Jang ◽

Inwoo Son ◽

Jongseong Kim ◽

Yongdoo Park

Keyword(s):

Tissue Engineering ◽

Extracellular Matrix ◽

Computational Modeling ◽

Cardiac Tissue ◽

Cardiac Development ◽

Heart Tissue ◽

Cardiac Tissue Engineering ◽

Cardiac Cells

Cardiac tissue engineering aims to generate in vivo-like functional tissue for the study of cardiac development, homeostasis, and regeneration. Since the heart is composed of various types of cells and extracellular matrix with a specific microenvironment, the fabrication of cardiac tissue in vitro requires integrating technologies of cardiac cells, biomaterials, fabrication, and computational modeling to model the complexity of heart tissue. Here, we review the recent progress of engineering techniques from simple to complex for fabricating matured cardiac tissue in vitro. Advancements in cardiomyocytes, extracellular matrix, geometry, and computational modeling will be discussed based on a technology perspective and their use for preparation of functional cardiac tissue. Since the heart is a very complex system at multiscale levels, an understanding of each technique and their interactions would be highly beneficial to the development of a fully functional heart in cardiac tissue engineering.

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075 Generation and quantification of oxidized squalene to develop an acne testing model in vitro based on skin tissue engineering

Journal of Investigative Dermatology ◽

10.1016/j.jid.2016.06.093 ◽

2016 ◽

Vol 136 (9) ◽

pp. S173 ◽

Cited By ~ 1

Author(s):

N. Esselin ◽

C. Capallere ◽

C. Meyrignac ◽

C. Plaza ◽

C. Coquet ◽

...

Keyword(s):

Tissue Engineering ◽

Skin Tissue ◽

Testing Model ◽

Skin Tissue Engineering

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Scaffolds in tissue engineering of blood vessels

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y10-073 ◽

2010 ◽

Vol 88 (9) ◽

pp. 855-873 ◽

Cited By ~ 60

Author(s):

Divya Pankajakshan ◽

Devendra K. Agrawal

Keyword(s):

Tissue Engineering ◽

Extracellular Matrix ◽

Blood Vessels ◽

Vascular Tissue ◽

Small Diameter ◽

Biological Scaffolds ◽

Hemodynamic Forces ◽

Biodegradable Scaffolds

Tissue engineering of small diameter (<5 mm) blood vessels is a promising approach for developing viable alternatives to autologous vascular grafts. It involves in vitro seeding of cells onto a scaffold on which the cells attach, proliferate, and differentiate while secreting the components of extracellular matrix that are required for creating the tissue. The scaffold should provide the initial requisite mechanical strength to withstand in vivo hemodynamic forces until vascular smooth muscle cells and fibroblasts reinforce the extracellular matrix of the vessel wall. Hence, the choice of scaffold is crucial for providing guidance cues to the cells to behave in the required manner to produce tissues and organs of the desired shape and size. Several types of scaffolds have been used for the reconstruction of blood vessels. They can be broadly classified as biological scaffolds, decellularized matrices, and polymeric biodegradable scaffolds. This review focuses on the different types of scaffolds that have been designed, developed, and tested for tissue engineering of blood vessels, including use of stem cells in vascular tissue engineering.

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