scholarly journals The active ingredients and mechanisms of Longchai Jiangxue Formula in treating PV, based on UPLC/Q-TOF-MS/MS, systematic pharmacology, and molecular biology validation

2021 ◽  
Vol 140 ◽  
pp. 111767
Author(s):  
Jing Ming ◽  
Weiyi Liu ◽  
Hongwei Wu ◽  
Yujin Li ◽  
Erpeng Yang ◽  
...  
Keyword(s):  
Molecular Biology ◽  
Active Ingredients ◽  
Tof Ms

The effect of Wogonoside on the expression of pulmonary fibrosis factor in Mycoplasma pneumoniae pneumonia

2020 ◽  
Author(s):  
Huihui Wang ◽  
Xiaoxi Wang ◽  
Zhimin Yang ◽  
Huixing Xu ◽  
Xin Wang ◽  
...  
Keyword(s):  
Mycoplasma Pneumoniae ◽  
Active Ingredient ◽  
A549 Cells ◽  
Scutellaria Baicalensis ◽  
Chinese Herbs ◽  
Affinity Constant ◽  
Active Ingredients ◽  
Pulmonary Interstitial Fibrosis ◽  
Mycoplasma Pneumoniae Pneumonia ◽  
Tof Ms

Abstract BACKGROUND Mycoplasma pneumoniae pneumonia (MPP) is an acute respiratory infectious pneumonia with pulmonary interstitial fibrosis. TGF-β1 is well accepted as the central mediator for fibrosis which promotes the formation of tissue fibrosis factor. Qinbaiqingfei pellet (Qinbai) has already been approved as the first effective new traditional Chinese medicine which can delay activities of Mycoplasma pneumoniae (M.pneumoniae) and protect lung epithelial cells in clinical trials. However, the mechanism of Qinbai inhibiting the expression of TGF-β1 is still unclear. METHODS The Chinese herbs in Qinbai were screened by surface plasmon resonance (SPR) and it was deteimined that Scutellaria baicalensis extracts in Qinbai showed the best binding with TGF-β1. Then the active ingredient whicn can be bound with TGF-β1 protein in the solution of Qinbai and Scutellaria baicalensis was isolated and analyzed by UPLC-Q-TOF-MS, which proved the active ingredient was Wogonoside. The affinity constant of Wogonoside and TGF-β1 protein was measured by SPR affinity analysis. A549 cells infected with M.pneumoniae were intervened by Wogonoside, and then the expression of TGF-β1 and Smad3 in A549 cells were analyzed by PCR and western blotting. RESULTS The results showed the bound effect of Scutellaria baicalensis and TGF-β1 was effective. The active ingredients which can be bound with TGF-β1 in the solution of Scutellaria baicalensis and Qinbai were obtained and analyzed to investigate the mechanism of Qinbai inhibiting the expression of TGF-β1. UPLC-Q-TOF-MS results showed that the active ingredients was Wogonoside. SPR affinity analysis showed that the affinity constant was 21.71 µM. Pharmacological experiments revealed that Wogonoside strongly inhibited the expression of TGF-β1 and Smad3 in A549 cells infected by M.pneumoniae. CONCLUSION Wogonoside in Qinbai can be bound with TGF-β1 and down-regulate the expression of lung fibrosis factors TGF-β1 and Smad3. The finding may improve our understanding the molecular mechanism of Qinbai mediating MPP and provide new sights into the future pharmacological investigation of Qinbai.

Screening of anti-complement active ingredients from Eucommia ulmoides Oliv. branches and their metabolism in vivo based on UHPLC-Q-TOF/MS/MS

2019 ◽  
Vol 1124 ◽  
pp. 26-36 ◽  
Author(s):  
Jia Jia ◽  
Mi Liu ◽  
Quan Wen ◽  
Mingzhen He ◽  
Hui Ouyang ◽  
...  
Keyword(s):  
Eucommia Ulmoides ◽  
Active Ingredients ◽  
Tof Ms

scholarly journals Rapid Identification and Assignation of the Active Ingredients in Fufang Banbianlian Injection Using HPLC-DAD-ESI-IT-TOF-MS

2016 ◽  
Vol 54 (7) ◽  
pp. 1225-1237 ◽  
Author(s):  
Sensen Li ◽  
Zongtao Lin ◽  
Haixiu Jiang ◽  
Lingkun Tong ◽  
Hong Wang ◽  
...  
Keyword(s):  
Rapid Identification ◽  
Active Ingredients ◽  
Tof Ms

scholarly journals Cosmeceuticals. Composition and effects

2021 ◽  
Vol 10 (3) ◽  
pp. 135-141
Author(s):  
Justyna Marwicka ◽  
◽  
Aleksandra Gałuszka ◽  
Małgorzata Kotwica ◽  
◽  
...  
Keyword(s):  
Molecular Biology ◽  
Human Skin ◽  
Scientific Literature ◽  
Popular Science ◽  
Active Ingredients ◽  
High Concentration ◽  
Professional Advice ◽  
Intense Action ◽  
Active Substances

Cosmeceuticals are preparations that are described in numerous scientific and popular science works as combining the features of a drug and a cosmetic. They are cosmetics, but unlike the traditional ones, they contain a high concentration of active ingredients, usually plant-based, obtained with the use of modern pharmacy, biotechnology and molecular biology techniques. The aim of the study was to present, based on scientific literature, cosmeceuticals as multi-ingredient products and their impact on human skin. Active substances contained in cosmeceuticals cause their more intense action than traditional cosmetics. Therefore, they should be used based on the professional advice of a cosmetologist, pharmacist or doctor.

Identification Techniques of Acetic Acid Bacteria: Comparison between MALDI-TOF MS and Molecular Biology Techniques

2017 ◽  
pp. 162-192 ◽  
Author(s):  
Cristina Andrés-Barrao ◽  
François Barja ◽  
Ruben Ortega Pérez ◽  
Marie-Louise Chappuis ◽  
Sarah Braito ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Molecular Biology ◽  
Acetic Acid Bacteria ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Identification Techniques ◽  
Tof Ms

scholarly journals Study of the active ingredients and mechanism of Sparganii rhizoma in gastric cancer based on HPLC-Q-TOF–MS/MS and network pharmacology

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Xiaona Lu ◽  
Yawei Zheng ◽  
Fang Wen ◽  
Wenjie Huang ◽  
Xiaoxue Chen ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Aqueous Extract ◽  
Target Prediction ◽  
Network Pharmacology ◽  
Chemical Components ◽  
Therapeutic Effects ◽  
Active Ingredients ◽  
Hub Genes ◽  
Anticancer Mechanism ◽  
Tof Ms

AbstractSparganii rhizoma (SL) has potential therapeutic effects on gastric cancer (GC), but its main active ingredients and possible anticancer mechanism are still unclear. In this study, we used HPLC-Q-TOF–MS/MS to comprehensively analyse the chemical components of the aqueous extract of SL. On this basis, a network pharmacology method incorporating target prediction, gene function annotation, and molecular docking was performed to analyse the identified compounds, thereby determining the main active ingredients and hub genes of SL in the treatment of GC. Finally, the mRNA and protein expression levels of the hub genes of GC patients were further analysed by the Oncomine, GEPIA, and HPA databases. A total of 41 compounds were identified from the aqueous extract of SL. Through network analysis, we identified seven main active ingredients and ten hub genes: acacetin, sanleng acid, ferulic acid, methyl 3,6-dihydroxy-2-[(2-hydroxyphenyl) ethynyl]benzoate, caffeic acid, adenine nucleoside, azelaic acid and PIK3R1, PIK3CA, SRC, MAPK1, AKT1, HSP90AA1, HRAS, STAT3, FYN, and RHOA. The results indicated that SL might play a role in GC treatment by controlling the PI3K-Akt and other signalling pathways to regulate biological processes such as proliferation, apoptosis, migration, and angiogenesis in tumour cells. In conclusion, this study used HPLC-Q-TOF–MS/MS combined with a network pharmacology approach to provide an essential reference for identifying the chemical components of SL and its mechanism of action in the treatment of GC.

scholarly journals The effect of Wogonoside on the expression of pulmonary fibrosis factor in Mycoplasma pneumoniae pneumonia

2020 ◽  
Author(s):  
Huihui Wang ◽  
Xiaoxi Wang ◽  
Zhimin Yang ◽  
Huixing Xu ◽  
Xin Wang ◽  
...  
Keyword(s):  
Mycoplasma Pneumoniae ◽  
Active Ingredient ◽  
A549 Cells ◽  
Scutellaria Baicalensis ◽  
Chinese Herbs ◽  
Affinity Constant ◽  
Active Ingredients ◽  
Pulmonary Interstitial Fibrosis ◽  
Mycoplasma Pneumoniae Pneumonia ◽  
Tof Ms

Abstract BACKGROUND Mycoplasma pneumoniae pneumonia (MPP) is an acute respiratory infectious pneumonia with pulmonary interstitial fibrosis. TGF-β1 is well accepted as the central mediator for fibrosis which promotes the formation of tissue fibrosis factor. Qinbaiqingfei pellet (Qinbai) has already been approved as the first effective new traditional Chinese medicine which can delay activities of Mycoplasma pneumoniae (M.pneumoniae) and protect lung epithelial cells in clinical trials. However, the mechanism of Qinbai inhibiting the expression of TGF-β1 is still unclear. METHODS The Chinese herbs in Qinbai were screened by surface plasmon resonance (SPR) and it was deteimined that Scutellaria baicalensis extracts in Qinbai showed the best binding with TGF-β1. Then the active ingredient whicn can be bound with TGF-β1 protein in the solution of Qinbai and Scutellaria baicalensis was isolated and analyzed by UPLC-Q-TOF-MS, which proved the active ingredient was Wogonoside. The affinity constant of Wogonoside and TGF-β1 protein was measured by SPR affinity analysis. A549 cells infected with M.pneumoniae were intervened by Wogonoside, and then the expression of TGF-β1 and Smad3 in A549 cells were analyzed by PCR and western blotting. RESULTS The results showed the bound effect of Scutellaria baicalensis and TGF-β1 was effective. The active ingredients which can be bound with TGF-β1 in the solution of Scutellaria baicalensis and Qinbai were obtained and analyzed to investigate the mechanism of Qinbai inhibiting the expression of TGF-β1. UPLC-Q-TOF-MS results showed that the active ingredients was Wogonoside. SPR affinity analysis showed that the affinity constant was 21.71 µM. Pharmacological experiments revealed that Wogonoside strongly inhibited the expression of TGF-β1 and Smad3 in A549 cells infected by M.pneumoniae. CONCLUSION Wogonoside in Qinbai can be bound with TGF-β1 and down-regulate the expression of lung fibrosis factors TGF-β1 and Smad3. The finding may improve our understanding the molecular mechanism of Qinbai mediating MPP and provide new sights into the future pharmacological investigation of Qinbai.

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