Effects of light sources on growth and carotenoid content of photosynthetic bacteria Rhodopseudomonas palustris

2012 ◽  
Vol 113 ◽  
pp. 315-318 ◽  
Author(s):  
Fu-Shiu Kuo ◽  
Yew-Hu Chien ◽  
Chang-Jiang Chen
Keyword(s):  
Photosynthetic Bacteria ◽  
Rhodopseudomonas Palustris ◽  
Carotenoid Content ◽  
Light Sources

scholarly journals The effects of Rhodopseudomonas palustris PSB06 and CGA009 with different agricultural applications on rice growth and rhizosphere bacterial communities

AMB Express ◽  
2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Luyun Luo ◽  
Pei Wang ◽  
Zhongying Zhai ◽  
Pin Su ◽  
Xinqiu Tan ◽  
...  
Keyword(s):  
Treatment Group ◽  
Bacterial Communities ◽  
Photosynthetic Bacteria ◽  
Rhodopseudomonas Palustris ◽  
Rice Seedling ◽  
Seedling Stage ◽  
Rice Rhizosphere ◽  
Rice Growth ◽  
Agricultural Applications ◽  
Rhizosphere Bacterial Communities

Abstract In recent years, the photosynthetic bacteria have been used widely in agriculture, but the effects of different agricultural applications on crop rhizosphere microorganism and crops are lack. In this study, we provide new insights into the structure and composition of the rice root-associated microbiomes as well as the effect on crop of the Rhodopseudomonas palustris(R. palustris) PSB06 and CGA009 at the rice seedling stage with seed immersion and root irrigation. Compare with CK group, the length of stem, the peroxidase (POD), and superoxide dismutase (SOD) activities in PSB06 treatment group was significantly higher, while the length of stem in CGA009 treatment group was significantly higher. The POD and SOD activities in CGA009 treatment groups only were higher slightly than the CK group. In the study, the dominant phyla were Proteobacteria (51.95–61.66%), Bacteroidetes (5.40–9.39%), Acidobacteria (4.50–10.52%), Actinobacteria (5.06–8.14%), Planctomycetes (2.90–4.48%), Chloroflexi (2.23–5.06%) and Firmicutes (2.38–7.30%), accounted for 87% bacterial sequences. The principal coordinate analysis (pCoA) and mantel results showed the two application actions of R. palustris CGA009 and PSB06 had significant effects on rice rhizosphere bacterial communities (p < 0.05). The PSB06 can significantly promote the rice growth and enhance stress resistance of rice at the seedling stage, while the R. palustris CGA009 has no significant effect on rice. Dissimilarity test and canonical correspondence analysis (CCA) results showed that the TN and pH were the key factors affecting rice rhizosphere bacterial community in the seedling stage. This study will provide some guidance advices for the study of the microecological regulation of photosynthetic bacteria on crops.

scholarly journals Efficient Ammonium Removal by Bacteria Rhodopseudomonas Isolated from Natural Landscape Water: China Case Study

Water ◽  
2018 ◽  
Vol 10 (8) ◽  
pp. 1107 ◽  
Author(s):  
Xuejiao Huang ◽  
Jiupai Ni ◽  
Chong Yang ◽  
Mi Feng ◽  
Zhenlun Li ◽  
...  
Keyword(s):  
Photosynthetic Bacteria ◽  
Novel Species ◽  
Fatty Acid Content ◽  
Rhodopseudomonas Palustris ◽  
Morphological Properties ◽  
Specific Primers ◽  
Natural Landscape ◽  
Landscape Water ◽  
Rdna Amplification

In this study, we isolated a strain of photosynthetic bacteria from landscape water located in Southwest University, Chongqing, China, and named it Smobiisys501. Smobiisys501 was Rhodopseudomonas sp. according to its cell morphological properties and absorption spectrum analysis of living cells. The analysis of the 16S rDNA amplification sequence with specific primers of photosynthetic bacteria showed that the homology between Smobiisys501 and Rhodopseudomonas sp. was 100%, and the alignment results of protein sequences of the bacterial chlorophyll Y subunit showed that Smobiisys501 and Rhodopseudomonas palustris were the most similar, with a similarity of more than 92%. However, Smobiisys501 could not utilize glucose and mannitol as a carbon source and had a low fatty acid content, which were different from the related strains of the genus Rhodopseudomonas. Moreover, the DNA-DNA relatedness was only 42.2 ± 3.3% between Smobiisys501 and the closest strain Rhodopseudomonas palustris. Smobiisys501 grew optimally at 30 °C and pH 7.0 in the presence of yeast extract, and it could efficiently remove ammonium (99.67% removal efficiency) from synthetic ammonium wastewater. All the results indicated that Smobiisys501 was a novel species of Rhodopseudomonas, with the ability to remove ammonium.

Pyruvic decarboxylase and acetoin formation in Athiorhodaceae

1973 ◽  
Vol 19 (9) ◽  
pp. 1137-1143 ◽  
Author(s):  
S. M. Hussain Qadri ◽  
D. S. Hoare
Keyword(s):  
Photosynthetic Bacteria ◽  
Bacterial Species ◽  
Rhodopseudomonas Palustris ◽  
Potassium Phosphate ◽  
Thiamine Pyrophosphate ◽  
Divalent Metal Ions ◽  
Sulfur Bacteria ◽  
Optimum Ph ◽  
Rhodospirillum Molischianum ◽  
Rhodomicrobium Vannielii

Studies with cell-free extracts of Rhodopseudomonas palustris strain Q showed that under anaerobic conditions pyruvate was decarboxylated with the stoichiometric formation of carbon dioxide. Other products of the reaction include acetaldehyde and acetoin (acetyl methyl carbinol), indicating the presence of pyruvic decarboxylase. A survey of purple non-sulfur bacteria demonstrated that pyruvic decarboxylase was also formed by R. gelatinosa and R. capsulata and Rhodospirillum molischianum but not by R. rubrum and R. spheroides and Rhodomicrobium vannielii. The enzyme had an optimum pH of 6.25 and an apparent Km of 1.22 × 10−2 M in potassium phosphate buffer (50 mM) at 30C. Pyruvic decarboxylase in photosynthetic bacteria required only thiamine pyrophosphate as a cofactor and it differed in this respect from the enzyme in yeast and in other bacteria which required divalent metal ions as well. Acetoin formation in the Athiorhodaceae, like that in yeast but unlike that in most bacterial species, involved the formation of acetaldehyde from pyruvate.

Immobilization and Ammonia Removal of Photosynthetic Bacteria

2012 ◽  
Vol 610-613 ◽  
pp. 311-314 ◽  
Author(s):  
Pei Rong Zhan ◽  
Wei Liu
Keyword(s):  
Electron Microscopy ◽  
Amino Acids ◽  
Photosynthetic Bacteria ◽  
Rhodopseudomonas Palustris ◽  
Carbon Sources ◽  
Water Environment ◽  
Photosynthetic Bacterium ◽  
Ammonia Removal ◽  
Fish Pond ◽  
Immobilized Microorganisms

The photosynthetic bacteria have been widely used in improving the water environment, especially for pollutant purification. A photosynthetic bacterium was isolated from fish pond sludge using various methods. The bacterium is rod-shaped and slightly curved, and they reproduce by budding. It grew anaerobically when exposed to light and aerobically in darkness. Based on electron microscopy, utilization of carbon sources and amino acids, and factors required for growth, the bacterium is identified as Rhodopseudomonas palustris. The R. palustris was immobilized using different carriers to increase its concentration and its targeted use. The results show that immobilization of the bacteria stabilized the ammonia removal and protected the bacteria from predation by plankton. The method is also easy to use and prolonged the purification effect in the reactor. The immobilized microorganisms are 30%–40% more effective than free bacteria in removing ammonia.

scholarly journals The Effect of Red & Blue Rich LEDs vs Fluorescent Light on Lollo Rosso Lettuce Morphology and Physiology

2021 ◽  
Vol 12 ◽  
Author(s):  
Laura Cammarisano ◽  
Iain S. Donnison ◽  
Paul R. H. Robson
Keyword(s):  
Broad Spectrum ◽  
New Technologies ◽  
Carotenoid Content ◽  
Fluorescent Light ◽  
Plant Responses ◽  
Light Sources ◽  
Light Emitting ◽  
Yield And Quality ◽  
Use Efficiency ◽  
Light Absorptance

The challenges of feeding an increasing population, an increasingly urban population and within an increasingly challenging global environment have focused ideas on new ways to grow food. Growing food in a controlled environment (CE) is not new but new technologies such as broad-spectrum LEDs and robotics are generating new opportunities. Growth recipes can be tailored to plant species in a CE and plasticity in plant responses to the environment may be utilized to make growth systems more efficient for improved yield and crop quality. Light use efficiency within CE must consider energy requirements, yield and impacts on quality. We hypothesized that understanding how plants change their morphology and physiology in response to light will allow us to identify routes to make light more efficient for delivery of high-quality produce. We focused on responses to light in Lollo rosso lettuce which produces compact, crinkly and highly pigmented leaves. We compared the spectra of the commonly used artificial light sources in indoor farming (compact fluorescence tubes, FL, and broad-spectrum light-emitting diodes, LEDs) at two irradiance levels (270 and 570 μmol m–2 s–1). We discovered LEDs (λP: 451, 634, and 665 nm) produced the same amount of produce for half the incident energy of FL (T5). At higher irradiances LEDs produced 9% thicker leaves, 13% larger rosettes and 15% greater carotenoid content. Leaves differed in light absorptance with plants grown under lower FL absorbing 30% less of mid-range wavelengths. We show that the relative efficiencies of LED and FL is a function of the irradiances compared and demonstrate the importance of understanding the asymptotes of yield and quality traits. Increasing our understanding of structural and biochemical changes that occur under different combination of wavelengths may allow us to better optimize light delivery, select for different ranges of plasticity in crop plants and further optimize light recipes.

scholarly journals Investigating and modelling the effect of light intensity on Rhodopseudomonas palustris growth

2021 ◽  
Author(s):  
Brandon Ross ◽  
Robert William McClelland Pott
Keyword(s):  
Experimental Data ◽  
Light Intensity ◽  
Photosynthetic Bacteria ◽  
Rhodopseudomonas Palustris ◽  
Photosynthetic Bacterium ◽  
Light Penetration ◽  
Tubular Photobioreactor ◽  
Photobioreactor Design ◽  
High Purity Hydrogen ◽  
Effect Of Light

Photosynthetic bacteria can be useful biotechnological tools – they produce a variety of valuable products, including high purity hydrogen, and can simultaneously treat recalcitrant wastewaters. However, while photobioreactors have been designed and modelled for photosynthetic algae and cyanobacteria, there has been less work on understanding the effect of light in photosynthetic bacterial fermentations. In order to design photobioreactors, and processes using these organisms, robust models of light penetration, utilisation and conversion are needed. This article uses experimental data from a tubular photobioreactor designed to focus in on light intensity effects, to model the effect of light intensity on the growth of Rhodopseudomonas palustris, a model photosynthetic bacterium. The work demonstrates that growth is controlled by light intensity, and that this organism does experience photoinhibition above 600 W/m2, which has implications for outdoor applications. Further, the work presents a model for light penetration in circular photobioreactors, which tends to be the most common geometry. The work extends the modelling tools for these organisms, and will allow for better photobioreactor design, and the integration of modelling tools in designing processes which use photosynthetic bacteria.

scholarly journals Clades of Photosynthetic Bacteria Belonging to the Genus Rhodopseudomonas Show Marked Diversity in Light-Harvesting Antenna Complex Gene Composition and Expression

mSystems ◽  
2015 ◽  
Vol 1 (1) ◽  
Author(s):  
Kathryn R. Fixen ◽  
Yasuhiro Oda ◽  
Caroline S. Harwood
Keyword(s):  
Photosynthetic Bacteria ◽  
Light Harvesting ◽  
Rhodopseudomonas Palustris ◽  
Phototrophic Bacteria ◽  
High Light ◽  
Gene Content ◽  
Low Light ◽  
Transcript Levels ◽  
Content Type ◽  
Light Intensities

ABSTRACT Rhodopseudomonas palustris is a phototrophic purple nonsulfur bacterium that adapts its photosystem to allow growth at a range of light intensities. It does this by adjusting the amount and composition of peripheral light-harvesting (LH) antenna complexes that it synthesizes. Rhodopseudomonas strains are notable for containing numerous sets of light-harvesting genes. We determined the diversity of LH complexes and their transcript levels during growth under high and low light intensities in 20 sequenced genomes of strains related to the species Rhodopseudomonas palustris. The data obtained are a resource for investigators with interests as wide-ranging as the biophysics of photosynthesis, the ecology of phototrophic bacteria, and the use of photosynthetic bacteria for biotechnology applications. Many photosynthetic bacteria have peripheral light-harvesting (LH) antenna complexes that increase the efficiency of light energy capture. The purple nonsulfur photosynthetic bacterium Rhodopseudomonas palustris produces different types of LH complexes under high light intensities (LH2 complex) and low light intensities (LH3 and LH4 complexes). There are multiple pucBA operons that encode the α and β peptides that make up these complexes. However, low-resolution structures, amino acid similarities between the complexes, and a lack of transcription analysis have made it difficult to determine the contributions of different pucBA operons to the composition and function of different LH complexes. It was also unclear how much diversity of LH complexes exists in R. palustris and affiliated strains. To address this, we undertook an integrative genomics approach using 20 sequenced strains. Gene content analysis revealed that even closely related strains have differences in their pucBA gene content. Transcriptome analyses of the strains grown under high light and low light revealed that the patterns of expression of the pucBA operons varied among strains grown under the same conditions. We also found that one set of LH2 complex proteins compensated for the lack of an LH4 complex under low light intensities but not under extremely low light intensities, indicating that there is functional redundancy between some of the LH complexes under certain light intensities. The variation observed in LH gene composition and expression in Rhodopseudomonas strains likely reflects how they have evolved to adapt to light conditions in specific soil and water microenvironments. IMPORTANCE Rhodopseudomonas palustris is a phototrophic purple nonsulfur bacterium that adapts its photosystem to allow growth at a range of light intensities. It does this by adjusting the amount and composition of peripheral light-harvesting (LH) antenna complexes that it synthesizes. Rhodopseudomonas strains are notable for containing numerous sets of light-harvesting genes. We determined the diversity of LH complexes and their transcript levels during growth under high and low light intensities in 20 sequenced genomes of strains related to the species Rhodopseudomonas palustris. The data obtained are a resource for investigators with interests as wide-ranging as the biophysics of photosynthesis, the ecology of phototrophic bacteria, and the use of photosynthetic bacteria for biotechnology applications.

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