Microbial community structures in different wastewater treatment plants as revealed by 454-pyrosequencing analysis

2012 ◽  
Vol 117 ◽  
pp. 72-79 ◽  
Author(s):  
Man Hu ◽  
Xiaohui Wang ◽  
Xianghua Wen ◽  
Yu Xia
Keyword(s):  
Wastewater Treatment ◽  
Microbial Community ◽  
454 Pyrosequencing ◽  
Wastewater Treatment Plants ◽  
Community Structures ◽  
Microbial Community Structures ◽  
Pyrosequencing Analysis

Revealing microbial community structures in large- and small-scale activated sludge systems by barcoded pyrosequencing of 16S rRNA gene

2012 ◽  
Vol 66 (10) ◽  
pp. 2155-2161 ◽  
Author(s):  
Purnika Damindi Ranasinghe ◽  
Hiroyasu Satoh ◽  
Mamoru Oshiki ◽  
Kenshiro Oshima ◽  
Wataru Suda ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Microbial Community ◽  
16S Rrna ◽  
Activated Sludge ◽  
Wastewater Treatment Plants ◽  
Small Scale ◽  
Rrna Gene ◽  
Community Structures ◽  
Microbial Community Structures ◽  
Insight Into

The diversity of bacterial groups in activated sludge from large- and small-scale wastewater treatment plants was explored by barcoded pyrosequencing of 16S rRNA gene. Activated sludge samples (three small and 17 large scale) were collected from 12 wastewater treatment plants to clarify precise taxonomy and relative abundances. DNA was extracted, and amplified by 4 base barcoded 27f/519r primer set. The 454 Titanium (Roche) pyrosequences were obtained and analyses performed by Quantitative Insight Into Microbial Ecology (QIIME) with around 100,000 reads. Sequence statistics were computed, while constructing a phylogenetic tree and heatmap. Computed results explained total microbial diversity at phylum and class level and resolution was further extended to Operational Taxonomic Unit (OTU) based taxonomic assignment for investigating community distribution based on individual sample. Composition of sequence reads were compared and microbial community structures for large- and small-scale treatment plants were identified as major phyla (Proteobacteria and Bacteroidetes) and classes (Betaproteobacteria and Bacteroidetes). Also, family level breakdowns were explained and differences in family Nitrospiraceae and phylum Actinobacteria found at their species level were also illustrated. Thus, the pyrosequencing method provides high resolution insight into microbial community structures in activated sludge that might have been unnoticed with conventional approaches.

Microbial community structures and functions of wastewater treatment systems in plateau and cold regions

2018 ◽  
Vol 249 ◽  
pp. 684-693 ◽  
Author(s):  
Dexin Fang ◽  
Gen Zhao ◽  
Xiaoyi Xu ◽  
Qian Zhang ◽  
Qiushi Shen ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Microbial Community ◽  
Community Structures ◽  
Cold Regions ◽  
Microbial Community Structures ◽  
Wastewater Treatment Systems

Application of molecular methods to microbial community analysis of activated sludge

2000 ◽  
Vol 42 (3-4) ◽  
pp. 17-22 ◽  
Author(s):  
M. Onuki ◽  
H. Satoh ◽  
T. Mino ◽  
T. Matsuo
Keyword(s):  
Microbial Community ◽  
Activated Sludge ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Wastewater Treatment Plants ◽  
Community Analysis ◽  
Molecular Methods ◽  
Microbial Community Analysis ◽  
Community Structures ◽  
Microbial Community Structures ◽  
Gradient Gel Electrophoresis

In the last decade, molecular biology has made significant progress, and innovative molecular methods have become available to analyze microbial community structures. Among them, we applied the FISH (Fluorescent in situ Hybridization) method to analyze activated sludge in wastewater treatment plants (WWTPs). As a result, domain- or division-level community structures in activated sludge were determined successfully without cultivation. We also applied the PCR (polymerase chain reaction) -DGGE (Denaturing Gradient Gel Electrophoresis) method for laboratory nitrifying sludge in order to investigate more detailed microbial community structure. By this method, genus- or species-level community structures were characterized well. This method was also found to be powerful for monitoring the change of microbial community structures. For example, the behavior of Nitrosomonas group was successfully detected in the reactor with nitrification by the PCR-DGGE method.

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