Microbial community composition of a down-flow hanging sponge (DHS) reactor combined with an up-flow anaerobic sludge blanket (UASB) reactor for the treatment of municipal sewage

2014 ◽  
Vol 151 ◽  
pp. 144-150 ◽  
Author(s):  
Kengo Kubota ◽  
Mikio Hayashi ◽  
Kengo Matsunaga ◽  
Akinori Iguchi ◽  
Akiyoshi Ohashi ◽  
...  
2016 ◽  
Vol 74 (2) ◽  
pp. 500-507 ◽  
Author(s):  
Nguyen Thi Thanh ◽  
Takahiro Watari ◽  
Tran Phuong Thao ◽  
Masashi Hatamoto ◽  
Daisuke Tanikawa ◽  
...  

In this study, granular sludge formation was carried out using an aluminum chloride supplement in an upflow anaerobic sludge blanket (UASB) reactor treating natural rubber processing wastewater. Results show that during the first 75 days after the start-up of the UASB reactor with an organic loading rate (OLR) of 2.65 kg-COD·m−3·day−1, it performed stably with a removal of 90% of the total chemical oxygen demand (COD) and sludge still remained in small dispersed flocs. However, after aluminum chloride was added at a concentration of 300 mg·L−1 and the OLR range was increased up to 5.32 kg-COD·m−3·day−1, the total COD removal efficiency rose to 96.5 ± 2.6%, with a methane recovery rate of 84.9 ± 13.4%, and the flocs began to form granules. Massively parallel 16S rRNA gene sequencing of the sludge retained in the UASB reactor showed that total sequence reads of Methanosaeta sp. and Methanosarcina sp., reported to be the key organisms for granulation, increased after 311 days of operation. This indicates that the microbial community structure of the retained sludge in the UASB reactor at the end of the experiment gave a good account of itself in not only COD removal, but also granule formation.


2020 ◽  
Vol 7 (2) ◽  
pp. 43 ◽  
Author(s):  
Matia Mainardis ◽  
Marco Buttazzoni ◽  
Daniele Goi

Up-flow anaerobic sludge blanket (UASB) reactor belongs to high-rate systems, able to perform anaerobic reaction at reduced hydraulic retention time, if compared to traditional digesters. In this review, the most recent advances in UASB reactor applications are critically summarized and discussed, with outline on the most critical aspects for further possible future developments. Beside traditional anaerobic treatment of soluble and biodegradable substrates, research is actually focusing on the treatment of refractory and slowly degradable matrices, thanks to an improved understanding of microbial community composition and reactor hydrodynamics, together with utilization of powerful modeling tools. Innovative approaches include the use of UASB reactor for nitrogen removal, as well as for hydrogen and volatile fatty acid production. Co-digestion of complementary substrates available in the same territory is being extensively studied to increase biogas yield and provide smooth continuous operations in a circular economy perspective. Particular importance is being given to decentralized treatment, able to provide electricity and heat to local users with possible integration with other renewable energies. Proper pre-treatment application increases biogas yield, while a successive post-treatment is needed to meet required effluent standards, also from a toxicological perspective. An increased full-scale application of UASB technology is desirable to achieve circular economy and sustainability scopes, with efficient biogas exploitation, fulfilling renewable energy targets and green-house gases emission reduction, in particular in tropical countries, where limited reactor heating is required.


RSC Advances ◽  
2016 ◽  
Vol 6 (55) ◽  
pp. 49995-50001 ◽  
Author(s):  
Min-Hua Cui ◽  
Dan Cui ◽  
Bin Liang ◽  
Thangavel Sangeetha ◽  
Ai-Jie Wang ◽  
...  

An up-flow anaerobic sludge blanket (UASB) reactor was developed to investigate the effect of azo dye loading rate on the decolorization performance and microbial community.


2012 ◽  
Vol 65 (2) ◽  
pp. 254-261 ◽  
Author(s):  
Rongjiu Shi ◽  
Ying Zhang ◽  
Weichao Yang ◽  
Hui Xu

The microbial community of a mesophilic lab-scale upflow anaerobic sludge blanket (UASB) reactor treating vitamin C biosynthesis wastewater at gradually elevated organic loading rates (OLRs) was characterized using 16S rDNA-based polymerase chain reaction-DGGE (denatured gradient gel electrophoresis) analysis. The DGGE fingerprints suggested that the elevated OLRs did not cause any significant changes in the microbial community. The predominant bacterial bands were affiliated with the Firmicutes (Clostridiales, four bands), Proteobacteria (Deltaproteobacteria, six bands), Bacteroidetes, and Synergistetes, respectively. All the archaeal bands were very similar to already known methanogenic species: Methanobacterium formicicum (two bands), Methanomethylovorans hollandica (one band) and Methanosaeta concilli (two bands), which belonged to the divisions Methanobacteria and Methanomicrobia, respectively.


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