Effects of lactic acid bacteria and molasses additives on the microbial community and fermentation quality of soybean silage

This experiment was conducted to determine the ensiling characteristics and microbial changes of fodder ramie silage treated without additive (Control), or with molasses (M), lactic acid bacteria (LAB), and mixtures of lactic acid bacteria and molasses (LABM). Triplicate samples were randomly opened on days 0, 3, 7, 14, 28 and 60 of ensiling for sampling and the contents were processed for quality assessment and laboratory analysis. Compared with control silage, addition of M and LABM decreased pH and butyric acid while increasing lactic acid during ensiling (P < 0.05). For the LAB treatment, the pH value declined slowly at initial days then kept relatively stable at about 5.39 and the concentration of lactic acid increased for the first 7 days then maintained stable until day 60. The control silage showed a rise in pH and a significant decline in lactic acid concentration at later stage. Microbial changes had similar trend during ensiling for all the treatments where the lactic acid bacteria increased at initial days then showed a decline at later stage. Furthermore, LAB treatment had the highest (P<0.05) lactic acid bacteria population at almost all ensiling periods. It was concluded that both M and LABM treatment can improve the fermentation quality of fodder ramie silage to some extent, but the effects of adding lactic acid bacteria still need further research.

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Effect of 3-Phenyllactic Acid and 3-Phenyllactic Acid-Producing Lactic Acid Bacteria on the Characteristics of Alfalfa Silage

Agriculture ◽

10.3390/agriculture10010010 ◽

2019 ◽

Vol 10 (1) ◽

pp. 10 ◽

Cited By ~ 1

Author(s):

Zhe Wu ◽

Shengyang Xu ◽

Ying Yun ◽

Tingting Jia ◽

Zhu Yu

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Gene Sequencing ◽

16S Rdna Gene ◽

Phenyllactic Acid ◽

Colony Forming Units ◽

Fermentation Quality ◽

Alfalfa Silage ◽

16S Rdna Gene Sequencing

In this study, an experiment was performed to evaluate the effect of lactic acid bacteria and 3-phenyllactic acid (PLA) on the fermentation quality and chemical composition of alfalfa silage. Several PLA-tolerant strains were screened from silages and identified. The selected strains (1 × 106 colony forming units/g fresh alfalfa) and PLA (1.0, 2.0, or 3.0 g/kg) were applied to alfalfa before ensiling. After 45 days of storage, the silages were unsealed and subjected to component analysis. Biochemical methods and 16S rDNA gene sequencing were used for the identification of the two strains as Lactobacillus plantarum. The characteristics of chemical and fermentation compounds indicated that PLA and the two strains efficiently improved the quality of the alfalfa silage. It can be concluded that the use of the strains and PLA can significantly improve the quality of silage.

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Effect of Addition of Chlorella and Lactic Acid Bacteria on Nutritive Value and Fermentation Quality of Fresh Rice Straw Silage

Journal of The Korean Society of Grassland and Forage Science ◽

10.5333/kgfs.2015.35.2.159 ◽

2015 ◽

Vol 35 (2) ◽

pp. 159-165 ◽

Cited By ~ 2

Author(s):

Ki Choon Choi ◽

Soundarrajan Ilavenil ◽

Mariadhas Valan Arasu ◽

Hyung-Su Park ◽

Won-Ho Kim

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Rice Straw ◽

Nutritive Value ◽

Fermentation Quality

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Potential Sustainable Properties of Microencapsulated Endophytic Lactic Acid Bacteria (KCC-42) in In-Vitro Simulated Gastrointestinal Juices and Their Fermentation Quality of Radish Kimchi

BioMed Research International ◽

10.1155/2018/6015243 ◽

2018 ◽

Vol 2018 ◽

pp. 1-10

Author(s):

Chae Eun Song ◽

Han Hyo Shim ◽

Palaniselvam Kuppusamy ◽

Young-IL Jeong ◽

Kyung Dong Lee

Keyword(s):

Acetic Acid ◽

Lactic Acid ◽

Lactic Acid Bacteria ◽

Organic Acids ◽

Succinic Acid ◽

Fermentation Medium ◽

Gastrointestinal Fluid ◽

Fermentation Quality

The objective of this study was to investigate alginate microencapsulated lactic acid bacteria (LAB) fermentation quality of radish kimchi sample and its potential survivability in different acidic and alkaline environments. Initially, we isolated 45 LAB strains. One of them showed fast growth pattern with potential probiotic and antifungal activities against Aspergillus flavus with a zone of inhibition calculated with 10, 8, 4mm for the 4th, 5th, and 6th day, respectively. Therefore, this strain (KCC-42) was chosen for microencapsulation with alginate biopolymer. It showed potential survivability in in-vitro simulated gastrointestinal fluid and radish kimchi fermentation medium. The survival rate of this free and encapsulated LAB KCC-42 was 6.85 × 105 and 7.48× 105 CFU/ml, respectively; the viability count was significantly higher than nonencapsulated LAB in simulated gastrointestinal juices (acid, bile, and pancreatin) and under radish kimchi fermentation environment. Kimchi sample added with this encapsulated LAB showed increased production of organic acids compared to nonencapsulated LAB sample. Also, the organic acids such as lactic acid, acetic acid, propionic acid, and succinic acid production in fermented kimchi were measured 59mM, 26mM, 14mM, and 0.6mM of g/DW, respectively. The production of metabolites such as lactic acid, acetic acid, and succinic acid and the bacteria population was high in microencapsulated LAB samples compared with free bacteria added kimchi sample. Results of this study indicate that microencapsulated LAB KCC-42 might be a useful strategy to develop products for food and healthcare industries.

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