Bioaugmentation with Diaphorobacter polyhydroxybutyrativorans to enhance nitrate removal in a poly (3-hydroxybutyrate-co-3-hydroxyvalerate)-supported denitrification reactor

2018 ◽  
Vol 263 ◽  
pp. 499-507 ◽  
Author(s):  
Shusong Zhang ◽  
Xingbin Sun ◽  
Xuming Wang ◽  
Tianlei Qiu ◽  
Min Gao ◽  
...  
Keyword(s):  
Nitrate Removal ◽  
Denitrification Reactor

scholarly journals Denitrification-Potential Evaluation and Nitrate-Removal-Pathway Analysis of Aerobic Denitrifier Strain Marinobacter hydrocarbonoclasticus RAD-2

Water ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 1298 ◽  
Author(s):  
Dedong Kong ◽  
Wenbing Li ◽  
Yale Deng ◽  
Yunjie Ruan ◽  
Guangsuo Chen ◽  
...  
Keyword(s):  
Nitrate Removal ◽  
Aerobic Denitrification ◽  
Transcriptional Level ◽  
16S Rrna Sequence ◽  
Recirculating Aquaculture ◽  
N Removal ◽  
Denitrification Reactor ◽  
Potential Alternative ◽  
Marinobacter Hydrocarbonoclasticus

An aerobic denitrifier was isolated from a long-term poly (3-hydroxybutyrate-co-3-hydroxyvalerate) PHBV-supported denitrification reactor that operated under alternate aerobic/anoxic conditions. The strain was identified as Marinobacter hydrocarbonoclasticus RAD-2 based on 16S rRNA-sequence phylogenetic analysis. Morphology was observed by scanning electron microscopy (SEM), and phylogenetic characteristics were analyzed with the API 20NE test. Strain RAD-2 showed efficient aerobic denitrification ability when using NO3−-N or NO2−-N as its only nitrogen source, while heterotrophic nitrification was not detected. The average NO3−-N and NO2−-N removal rates were 6.47 mg/(L·h)and 6.32 mg/(L·h), respectively. Single-factor experiments indicated that a 5:10 C/N ratio, 25–40 °C temperature, and 100–150 rpm rotation speed were the optimal conditions for aerobic denitrification. Furthermore, the denitrifying gene napA had the highest expression on a transcriptional level, followed by the denitrifying genes nirS and nosZ. The norB gene was found to have significantly low expression during the experiment. Overall, great aerobic denitrification ability makes the RAD-2 strain a potential alternative in enhancing nitrate management for marine recirculating aquaculture system (RAS) practices.

Nitrate removal with lateral flow sulphur autotrophic denitrification reactor

2014 ◽  
Vol 35 (21) ◽  
pp. 2692-2697 ◽  
Author(s):  
Xiaomei Lv ◽  
Mingfei Shao ◽  
Ji Li ◽  
Chuanbo Xie
Keyword(s):  
Nitrate Removal ◽  
Lateral Flow ◽  
Autotrophic Denitrification ◽  
Denitrification Reactor

scholarly journals PENGOLAHAN AIR LINDI DENGAN PROSES BIOFILTER ANAEROB-AEROB DAN DENITRIFIKASI

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Nusa Idaman Said ◽  
Dinda Rita Krishumartani Hartaja
Keyword(s):  
Removal Efficiency ◽  
Retention Time ◽  
Nitrate Removal ◽  
Quality Standards ◽  
Ammonia Removal ◽  
Leachate Treatment ◽  
Denitrification Reactor ◽  
Aerobic Reactor ◽  
Environment Agency ◽  
Denitrification Process

Most of the leachate treatment in Indonesia using pond system, that is maturation ponds, anaerobic ponds, stabilization ponds, and continued using wetland. The weakness of this technology is long retention time (between 30-50 days), thus the building a pond requires a wide area. In addition, the processed leachate is over quality standards to be discharged into the environment agency. To overcome these problems, one alternative is to use a combination of processing leachate within anaerobic-aerobic biofilter and denitrification. The technology is expected to shorten the residence time, so that the land required for the processing of leachate is not too extensive . The processed leachate is also expected to meet the quality standards are allowed to be discharged into the environment. Leachate treatment using anaerobic - aerobic biofilter and the denitrification process with a total hidraulic retention time of 12 day, the retention time in the anaerobic reactor 8 ( eight ) days , the retention time in the aerobic reactor 3 (three) days and retention time in the denitrification reactor 1 (one) day can be generated COD removal efficiency of 97 %, ammonia removal efficiency of 97.56 %, TSS removal  efficiency 87.5 % , and nitrate removal efficiency of 86.4 % Keywords : Anaerob-aerob biofilter, denitrification, leachate.

Short and Long-Term Responses to Changes in Hydraulic Loading in a Fixed Denitrifying Biofilm

1992 ◽  
Vol 26 (3-4) ◽  
pp. 535-544
Author(s):  
M. M. de Mendonca ◽  
J. Silverstein ◽  
N. E. Cook
Keyword(s):  
Field Experiments ◽  
Nitrate Removal ◽  
Short Term ◽  
Packed Tower ◽  
Hydraulic Loading ◽  
Denitrification Reactor ◽  
Water Field ◽  
Short And Long Term ◽  
Term Response

The long- and short-term effects of hydraulic loading were investigated in a pilot packed tower bioreactor used for denitrifying a groundwater used for drinking water. Field experiments were conducted at hydraulic loading rates of 3.63 and 7.24 m3/hr/m2. The long-term (21 day average) performance of the denitrification reactor and the short-term response (48 hour) to regular air scour are reported here. In summary, an increase in hydraulic loading resulted in a long-term increase in the half-order denitrification rate constant, so that, on average, nitrate removal was maintained in the 5.2 m tower. At the lower hydraulic loading rate (3.63 m3/hr/m2) denitrification performance in the first 48 hours after air scour was maintained; however at twice the hydraulic loading, several days were required to restore the biofilm performance to long-term average levels.

Denitrification performance and microbial community dynamics in a denitrification reactor as revealed by high-throughput sequencing

2016 ◽  
Vol 17 (4) ◽  
pp. 940-946 ◽  
Author(s):  
Sevgi Demirel
Keyword(s):  
High Throughput Sequencing ◽  
Community Dynamics ◽  
Nitrate Removal ◽  
Fixed Bed ◽  
Adaptation Period ◽  
Column Reactor ◽  
Operational Conditions ◽  
Microbial Profile ◽  
Microbial Community Dynamics ◽  
Denitrification Reactor

The dynamics of the bacterial community associated with the denitrification process in a fixed bed column reactor (FBCR) were investigated using 454-pyrosequencing methodology. A FBCR filled with elemental sulfur and limestone was operated for about 94 days under autotrophic and mixotrophic (autotrophic + heterotrophic) conditions at 30 °C. Efficient simultaneous bromate and nitrate removal was achieved at feed concentrations of 500 µg/L bromate and 45 mg/L nitrate under autotrophic and mixotrophic conditions. Operational taxonomic units-based analysis (97% similarity cut-off) of bioreactor samples (three periods) revealed that the microbial diversity changed regardless of operational conditions. Sulfurimonas spp. was dominant in the reactor at the adaptation stage. Thiobacillus denitrificans is a chemolithoautotrophic bacterium that is capable of the oxidation of inorganic sulfur compounds. After the adaptation period, the microbial profile changed such that Spirochaetacea spp. and Denitratisoma spp. were major species in the column reactor. After 60 d of operation, Hyphomicrobium vulgare became dominant due to the mixotrophic denitrification conditions.

EQUILIBRIUM AND KINETICS STUDY OF NITRATE REMOVAL FROM WATER BY PUROLITE A520-E RESIN

2012 ◽  
Vol 11 (1) ◽  
pp. 37-45 ◽  
Author(s):  
Liliana Lazar ◽  
Laura Bulgariu ◽  
Anca Ceica ◽  
Igor Cretescu ◽  
Ion Balasanian
Keyword(s):  
Nitrate Removal ◽  
Kinetics Study ◽  
Equilibrium And Kinetics

Optimal operations for groundwater denitrification of drinking water using heterotrophic biological denitrification process

2006 ◽  
Vol 6 (2) ◽  
pp. 125-130
Author(s):  
C.-H. Hung ◽  
K.-H. Tsai ◽  
Y.-K. Su ◽  
C.-M. Liang ◽  
M.-H. Su ◽  
...  
Keyword(s):  
Drinking Water ◽  
Removal Efficiency ◽  
Nitrate Removal ◽  
Drinking Water Treatment ◽  
Nitrate Content ◽  
Source Water ◽  
Nitrogen Gas ◽  
Negative Effect ◽  
Denitrification Process ◽  
Heterotrophic Denitrification

Due to the extensive application of artificial nitrogen-based fertilizers on land, groundwater from the central part of Taiwan faces problems of increasing concentrations of nitrate, which were measured to be well above 30 mg/L all year round. For meeting the 10 mg/L nitrate standard, optimal operations for a heterotrophic denitrification pilot plant designed for drinking water treatment was investigated. Ethanol and phosphate were added for bacteria growing on anthracite to convert nitrate to nitrogen gas. Results showed that presence of high dissolved oxygen (around 4 mg/L) in the source water did not have a significantly negative effect on nitrogen removal. When operated under a C/N ratio of 1.88, which was recommended in the literature, nitrate removal efficiency was measured to be around 70%, sometimes up to 90%. However, the reactor often underwent severe clogging problems. When operated under C/N ratio of 1.0, denitrification efficiency decreased significantly to 30%. Finally, when operated under C/N ratio of 1.5, the nitrate content of the influent was almost completely reduced at the first one-third part of the bioreactor with an overall removal efficiency of 89–91%. Another advantage for operating with a C/N ratio of 1.5 is that only one-third of the biosolids was produced compared to a C/N value of 1.88.

Phosphate Elimination in a USB Denitrification Reactor

1991 ◽  
Vol 24 (10) ◽  
pp. 335-336
Author(s):  
J. de Bruin ◽  
A. Klapwijk ◽  
I. W. Koster ◽  
H. M. M. Bosgoed
Keyword(s):  
Denitrification Reactor
Sign in / Sign up

Export Citation Format

Share Document