An integrated semi-continuous culture to treat original swine wastewater and fix carbon dioxide by an indigenous Chlorella vulgaris MBFJNU-1 in an outdoor photobioreactor

Chlorella vulgaris is one of the promising microalgae strains that can produce high yield of bio-oils. The C. vulgaris was pretreated with microwave irradiation prior to extraction using supercritical carbon dioxide (SCCO2). Fourier transform infrared spectroscopy (FTIR) analysis showed microwave irradiation pretreatment does not affect the material composition of C. vulgaris. Scanning electron microscopy (SEM) of the microwave irradiation pretreated microalgae showed an agglomeration of the cells with the cells shape became distorted due to rupturing of the cell walls. Optimization of the SCCO2 process parameters (pressure, temperature and CO2 flow rate) was performed by using response surface methodology (RSM) with central composite design (CCD). Two factors significantly affecting the extraction yield were temperature and pressure. The model equation also predicted the optimum condition for the SCCO2 (without microwave pretreatment) at 70 , 5676 psi and 7 sL/ min while optimum condition for SCCO2 (microwave irradiation pretreatment) at 63 , 5948 psi and 10 sL/ min. High amount of saturated fatty acids (SFA), monounsaturated fatty acids (MUFA), -linolenic acid and palmitoleic acid were found in the extracted oil with microwave irradiation pre-treatment sample. In addition, the polyunsaturated fatty acids (PUFA) content in the microwave irradiation pretreated oil was considerably low and is desirable for biodiesel production.

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Impact of Cultivation Condition and Media Content on Chlorella vulgaris Composition

Advanced Pharmaceutical Bulletin ◽

10.15171/apb.2019.022 ◽

2019 ◽

Vol 9 (2) ◽

pp. 182-194 ◽

Cited By ~ 5

Author(s):

Yunes Panahi ◽

Ahmad Yari Khosroushahi ◽

Amirhossein Sahebkar ◽

Hamid Reza Heidari

Keyword(s):

Carbon Dioxide ◽

Chlorella Vulgaris ◽

Nutritional Supplements ◽

Growth Efficiency ◽

Source Material ◽

Cultivation Condition ◽

Media Content ◽

Specific Production ◽

Nutrient Requirements ◽

Promising Source

Microalgae are a source material in food, pharmacy, and cosmetics industries for producing various products including high-protein nutritional supplements, synthetic pharmaceuticals, and natural colors. A promising algal source for such productions is Chlorella vulgaris which contains a considerable protein content. Similar to other microalgae, its desirability is minimal nutrient requirements since they are unicellular, photosynthetic, and fast-growing microorganisms. Another propitious option to be produced by C. vulgaris is biodiesel, since it is rich in oil too. Besides, algal well thriving in presence of increased amount of carbon dioxide makes them a practicable alternative biofuel resource without some problems of the traditional ones. At the same time, C. vulgaris is also a promising source for nutraceuticals such as amino acids, vitamins, and antioxidants. This review aims to discuss the conditions need to be observed for achieving a favorable growth efficiency of the C. vulgaris, as well as targeted productions such as biomass, antioxidant, and biofuel. Additionally, different approaches to induce any specific production are also considered comprehensively.

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Examining the impact of carbon dioxide levels and modulation of resulting hydrogen peroxide in Chlorella vulgaris

Algal Research ◽

10.1016/j.algal.2021.102492 ◽

2021 ◽

Vol 60 ◽

pp. 102492

Author(s):

Chien-Ting Li ◽

Kevin Trigani ◽

Cristal Zuñiga ◽

Richard Eng ◽

Elizabeth Chen ◽

...

Keyword(s):

Carbon Dioxide ◽

Hydrogen Peroxide ◽

Chlorella Vulgaris ◽

Carbon Dioxide Levels ◽

The Impact

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Carbon Dioxide Fixation and Conidiation in Fusarium culmorum Grown in Continuous Culture

Journal of General Microbiology ◽

10.1099/00221287-99-1-59 ◽

1977 ◽

Vol 99 (1) ◽

pp. 59-68 ◽

Cited By ~ 5

Author(s):

R. LARMOUR ◽

R. MARCHANT

Keyword(s):

Carbon Dioxide ◽

Continuous Culture ◽

Fusarium Culmorum ◽

Carbon Dioxide Fixation

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Effect of carbon dioxide on growth of Pseudomonas putida ATCC 11172 on asparagine, citrate, glucose, and lactate in batch and continuous culture

Canadian Journal of Microbiology ◽

10.1139/m85-143 ◽

1985 ◽

Vol 31 (9) ◽

pp. 763-766 ◽

Cited By ~ 5

Author(s):

Göran Molin

Keyword(s):

Carbon Dioxide ◽

Growth Rate ◽

Pseudomonas Putida ◽

Continuous Culture ◽

Specific Growth ◽

Carbon Sources ◽

Maximum Specific Growth Rate ◽

The Other ◽

Continuous Cultures ◽

Batch Cultures

The growth of Pseudomonas putida ATCC 11172 on L-asparagine, citrate, D-glucose, and L-lactate was followed in air and in 40% CO2 + air, using batch and carbon-limited continuous cultures. Batch cultures in air utilized a mixture of the carbon sources simultaneously. However, a change to 40% CO2 favoured the utilization of glucose. The maximum specific growth rate (μmax) in air was about 0.3 h−1 on glucose and 0.6 h−1 on the other carbon sources. In CO2, the μmax for glucose was reduced by 16% compared with almost 60–70% for the others. An order of preference for the different carbon sources in continuous cultures was determined by comparing the dilution rates at which the different carbon sources started to appear in the effluent. Glucose was the first compound to appear as the dilution rate increased (lowest preference when grown in air). In 40% CO2, the μmax for glucose was slightly higher than the others and the recorded preference for glucose in continuous culture was equal to that for citrate but was somewhat lower than that of lactate and asparagine. D-Gluconate and glucono-δ-lactone were produced as a step in the utilization of glucose. The D-gluconate production was enhanced by CO2.

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