Disposable amperometric immunosensing strips fabricated by Au nanoparticles-modified screen-printed carbon electrodes for the detection of foodborne pathogen Escherichia coli O157:H7

Escherichia coli O157:H7 ( E. coli O157:H7) is a dangerous foodborne pathogen, mainly found in beef, milk, fruits, and their products, causing harm to human health or even death. Therefore, the detection of E. coli O157:H7 in food is particularly important. In this paper, we report a lateral flow immunoassay strip (LFIS) based on aggregation-induced emission (AIE) material labeling antigen as a fluorescent probe for the rapid detection of E. coli O157:H7. The detection sensitivity of the strip is 105 CFU/mL, which is 10 times higher than that of the colloidal gold test strip. This method has good specificity and stability and can be used to detect about 250 CFU of E. coli O157:H7 successfully in 25 g or 25 mL of beef, jelly, and milk. AIE-LFIS might be valuable in monitoring food pathogens for rapid detection.

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A Comparison of the Survival in Feces and Water of Escherichia coli O157:H7 Grown under Laboratory Conditions or Obtained from Cattle Feces

Journal of Food Protection ◽

10.4315/0362-028x-69.1.6 ◽

2006 ◽

Vol 69 (1) ◽

pp. 6-11 ◽

Cited By ~ 41

Author(s):

L. SCOTT ◽

P. McGEE ◽

J. J. SHERIDAN ◽

B. EARLEY ◽

N. LEONARD

Keyword(s):

Escherichia Coli ◽

Foodborne Pathogen ◽

Hemorrhagic Colitis ◽

Contaminated Water ◽

Escherichia Coli O157 ◽

E Coli ◽

Cattle Feces ◽

Oral Inoculation ◽

Before And After ◽

Uremic Syndrome

Escherichia coli O157:H7 is an important foodborne pathogen that can cause hemorrhagic colitis and hemolytic uremic syndrome. Cattle feces and fecally contaminated water are important in the transmission of this organism on the farm. In this study, the survival of E. coli O157:H7 in feces and water was compared following passage through the animal digestive tract or preparation in the laboratory. Feces were collected from steers before and after oral inoculation with a marked strain of E. coli O157:H7. Fecal samples collected before cattle inoculation were subsequently inoculated with the marked strain of E. coli O157:H7 prepared in the laboratory. Subsamples were taken from both animal and laboratory-inoculated feces to inoculate 5-liter volumes of water. E. coli O157:H7 in feces survived up to 97 days, and survival was not affected by the method used to prepare the inoculating strain. E. coli O157:H7 survived up to 109 days in water, and the bacteria collected from inoculated cattle were detected up to 10 weeks longer than the laboratory-prepared culture. This study suggests that pathogen survival in low-nutrient conditions may be enhanced by passage through the gastrointestinal tract.

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Rapid and accurate detection of Escherichia coli O157:H7 in beef using microfluidic wax-printed paper-based ELISA

The Analyst ◽

10.1039/d0an00224k ◽

2020 ◽

Vol 145 (8) ◽

pp. 3106-3115 ◽

Cited By ~ 4

Author(s):

Yanan Zhao ◽

Dexin Zeng ◽

Chao Yan ◽

Wei Chen ◽

Jianluan Ren ◽

...

Keyword(s):

Escherichia Coli ◽

Foodborne Pathogens ◽

Point Of Care ◽

Foodborne Pathogen ◽

Escherichia Coli O157 ◽

Good Sensitivity ◽

Point Of Care Testing ◽

Accurate Detection ◽

New Ideas ◽

Sensitivity Specificity

Escherichia coli O157:H7 is a severe foodborne pathogen. Paper-based ELISA can rapidly and accurately detect E.coli O157:H7 in beef. The method has good sensitivity, specificity and repeatability. It is suitable for point-of-care testing and offers new ideas for the detection of other foodborne pathogens.

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Incidence and Toxin Production Ability of Escherichia coli O157:H7 Isolated from Cattle Trucks†

Journal of Food Protection ◽

10.4315/0362-028x-70.10.2383 ◽

2007 ◽

Vol 70 (10) ◽

pp. 2383-2385 ◽

Cited By ~ 11

Author(s):

E. P. CUESTA ALONSO ◽

S. E. GILLILAND ◽

C. R. KREHBIEL

Keyword(s):

Escherichia Coli ◽

Potential Risk ◽

Foodborne Pathogen ◽

Toxin Production ◽

Escherichia Coli O157 ◽

Foodborne Illnesses ◽

E Coli

Twelve cattle trucks were analyzed for the presence of Escherichia coli O157:H7. Three of them had been washed prior to arrival, and the others had not. Seventy-five percent of the trailers were positive for the presence of this foodborne pathogen. A total of 54 cultures were isolated and identified as E. coli O157:H7, all from the trucks that had not been cleaned. Most of the cultures (96.4%) produced Shiga-like toxin (verotoxin). No E. coli O157:H7 was detected in cattle trucks that were cleaned before arrival at the cattle pens. The incidence of E. coli O157:H7 in transport trailers increases the potential risk of contamination of cattle and transmission from farms to feedlots and to packing plants. This contamination increases the potential of contamination of meat during harvest and the risk of foodborne illnesses.

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Growth of Escherichia coli O157:H7 at Fluctuating Incubation Temperatures

Journal of Food Protection ◽

10.4315/0362-028x-58.12.1307 ◽

1995 ◽

Vol 58 (12) ◽

pp. 1307-1313 ◽

Cited By ~ 24

Author(s):

KATHLEEN T. RAJKOWSKI ◽

BENNE S. MARMER

Keyword(s):

Escherichia Coli ◽

Foodborne Pathogen ◽

Brain Heart Infusion ◽

Growth Curves ◽

Escherichia Coli O157 ◽

Growth Responses ◽

Growth Data ◽

Fluctuating Temperature ◽

E Coli ◽

Fluctuating Temperatures

Temperature abuse of foods is often transitory and little information is available describing the response of the foodborne pathogen, Escherichia coli O157:H7, to nonisothermal and/or fluctuating temperature storage. Growth responses were determined for a mixture of three E. coli O157:H7 strains in brain heart infusion (BHI) broth as a function of temperature (static and fluctuating), initial pH (5, 6, and 7), and NaCl content (0.5, 1, 2, and 3%). Five 6-h “square-wave” fluctuating temperature regimes were used: 4 to 12, 4 to 19, 4 to 28, 8 to 19, and 12 to 28°C and compared with growth at 8, 10, 12, 19, and 28°C. The growth curves obtained from fitting the Gompertz equation for the fluctuating temperatures were compared to those obtained for the static temperatures. Increased NaCl concentration decreased growth temperature both for the fluctuating temperature growth curves and the static growth data. The cells grew or remained viable for up to 21 days under all conditions and fluctuating temperatures. Growth kinetics at fluctuating temperatures more closely approximated the higher temperature than the midpoint temperature of each cyclic range. The results indicate that transitory abuse could lead to more rapid growth than expected of E. coli O157:H7 in foods and that given sufficient time E. coli O157:H7 can grow at as low as 8°C.

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CdS quantum dots/Au nanoparticles/ZnO nanowire array for self-powered photoelectrochemical detection of Escherichia coli O157:H7

Biosensors and Bioelectronics ◽

10.1016/j.bios.2019.111843 ◽

2020 ◽

Vol 149 ◽

pp. 111843 ◽

Cited By ~ 6

Author(s):

Xiuxiu Dong ◽

Zengliang Shi ◽

Chunxiang Xu ◽

Chi Yang ◽

Feng Chen ◽

...

Keyword(s):

Escherichia Coli ◽

Quantum Dots ◽

Au Nanoparticles ◽

Nanowire Array ◽

Escherichia Coli O157 ◽

Cds Quantum Dots ◽

Zno Nanowire ◽

Self Powered

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Escherichia coli O157:H7 Prevalence in Fecal Samples of Cattle from a Southeastern Beef Cow-Calf Herd

Journal of Food Protection ◽

10.4315/0362-028x-66.10.1778 ◽

2003 ◽

Vol 66 (10) ◽

pp. 1778-1782 ◽

Cited By ~ 19

Author(s):

D. G. RILEY ◽

J. T. GRAY ◽

G. H. LONERAGAN ◽

K. S. BARLING ◽

C. C. CHASE

Keyword(s):

Escherichia Coli ◽

Control Point ◽

Foodborne Pathogen ◽

Beef Cows ◽

Escherichia Coli O157 ◽

Fecal Samples ◽

E Coli ◽

Critical Control Point ◽

Cow Calf ◽

Culture Positive

The proportion of fecal samples culture-positive for Escherichia coli O157:H7 was determined for samples collected from 296 beef cows on pasture in a single Florida herd in October, November, and December 2001. The overall proportion of samples that cultured positive was 0.03. The proportion of cows that were culture-positive on at least one occasion was 0.091. No effect of pregnancy status or nutritional regimen on the proportion of culture-positive samples for E. coli O157:H7 was detected. We detected a breed effect on the shedding of E. coli O157, with Romosinuano cows having a lower (P < 0.01) proportion of samples culture-positive than Angus or Brahman cows. This difference might have resulted from the presence of confounding variables; however, it also might represent evidence of breed-to-breed genetic variation in E. coli O157 shedding. Further research is warranted to evaluate breed as a possible risk factor for shedding of this important foodborne pathogen. Further substantiated findings could indicate that breed is a cow-calf–level critical control point of E. coli O157:H7.

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