Multipurpose high-throughput filtering microarrays (HiFi) for DNA and protein assays

2010 ◽  
Vol 26 (4) ◽  
pp. 1142-1151 ◽  
Author(s):  
Gaelle C. Le Goff ◽  
Cloé Desmet ◽  
Jean-Charles Brès ◽  
Dominique Rigal ◽  
Loïc J. Blum ◽  
...  
Author(s):  
Maura C. Kibbey ◽  
David MacAllan ◽  
James W. Karaszkiewicz

IGEN's ORIGEN® technology, which is based on electrochemiluminescence, has been adopted by a number of research and bioanalytical laboratories who have recognized its exquisite sensitivity, high precision, wide dynamic range, and flexibility in formatting a wide variety of applications. IGEN's M-SERIES™ marks the introduction of the second generation of detection systems employing the ORIGEN technology specifically repackaged to address the needs of the high throughput laboratories involved in drug discovery. Assays are formatted without wash steps. Users realize the high performance of a heterogeneous technology with the convenience of a homogeneous format. The M-SERIES platform can address enzymatic assays (kinases, proteases, helicases, etc.), receptor-ligand or protein-protein assays, immunoassays, quantitation of nucleic acids, as well as other applications. Recent assay formats will be explored in detail.


2018 ◽  
Author(s):  
Curtis J Layton ◽  
Peter L McMahon ◽  
William J Greenleaf

SummaryHigh-throughput DNA sequencing techniques have enabled diverse approaches for linking DNA sequence to biochemical function. In contrast, assays of protein function have substantial limitations in terms of throughput, automation, and widespread availability. We have adapted an Illumina high-throughput sequencing chip to display an immense diversity of ribosomally-translated proteins and peptides, and then carried out fluorescence-based functional assays directly on this flow cell, demonstrating that a single, widely-available high-throughput platform can perform both sequencing-by-synthesis and protein assays. We quantified the binding of the M2 anti-FLAG antibody to a library of 1.3×104 variant FLAG peptides, exploring non-additive effects of combinations of mutations and discovering a “superFLAG” epitope variant. We also measured the enzymatic activity of 1.56×105 molecular variants of full-length of human O6-alkylguanine-DNA alkyltransferase (SNAP-tag). This comprehensive corpus of catalytic rates linked to amino acid sequence perturbations revealed amino acid interaction networks and cooperativity, linked positive cooperativity to structural proximity, and revealed ubiquitous positively-cooperative interactions with histidine residues.


2021 ◽  
Author(s):  
xuemeng zhang ◽  
Wei Wang ◽  
Richard N. Zare ◽  
Qianhao Min

High-throughput identification and quantification of protein/peptide biomarkers from biofluids in a label-free manner is achieved by interfacing bio-affinity arrays (BAAs) with nano-electrospray desorption electrospray ionization mass spectrometry (nano-DESI-MS). A wide...


2019 ◽  
Vol 73 (5) ◽  
pp. 1075-1082.e4 ◽  
Author(s):  
Curtis J. Layton ◽  
Peter L. McMahon ◽  
William J. Greenleaf

2012 ◽  
Vol 14 (4) ◽  
pp. 651-657 ◽  
Author(s):  
S. W. Birtwell ◽  
G. R. Broder ◽  
P. L. Roach ◽  
H. Morgan

2007 ◽  
Vol 177 (4S) ◽  
pp. 52-53
Author(s):  
Stefano Ongarello ◽  
Eberhard Steiner ◽  
Regina Achleitner ◽  
Isabel Feuerstein ◽  
Birgit Stenzel ◽  
...  

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