A simple electrochemical aptasensor for ultrasensitive protein detection using cyclic target-induced primer extension

2012 ◽  
Vol 36 (1) ◽  
pp. 12-17 ◽  
Author(s):  
Wei Cheng ◽  
Shijia Ding ◽  
Qing Li ◽  
Tianxiao Yu ◽  
Yibin Yin ◽  
...  
Keyword(s):  
Protein Detection ◽  
Primer Extension ◽  
Electrochemical Aptasensor

A New Electrochemical Aptasensor for Protein Detection Based on Target Protein-Induced Strand Displacement

2013 ◽  
Vol 753-755 ◽  
pp. 2113-2116 ◽  
Author(s):  
Han Feng Cui ◽  
Hao Fan ◽  
Yan Lin
Keyword(s):  
Protein Detection ◽  
Thiol Group ◽  
Target Protein ◽  
Film Electrode ◽  
Electrochemical Aptasensor ◽  
Strand Displacement ◽  
Au Electrode ◽  
Mercury Film Electrode ◽  
Detection Probe ◽  
Electrochemical Signal

A sensitive electrochemical aptasensor for detection of thrombin based on target protein-induced strand displacement is presented. For this proposed aptasensor, dsDNA which is from immobilized probe DNA (IP) which has a hybridized with aptamer(Apt) initially was immobilized on the Au electrode through thiol group of IP，while a DNA labeled with PdS nanoparticles was used as a detection probe (DP-PdS). When Au electrode immersed into solution which contain target protein and DP-PdS, the aptamer preferred to form G-quarter structure with the present target protein and dsDNA was released into immobilized probe DNA (IP) which could be hybridized with DP-PdS. After dissolving PdS from electrode, a mercury-film electrode was used for electrochemical detection of these Pd2+ ions which offered excellent electrochemical signal transduction. Detection of thrombin was specific without being affected by the coexistence of other proteins such as BSA and lysozyme, and as low as 2.3×10-11 mol/L of thrombin was detected.

Molybdenum disulfide sphere-based electrochemical aptasensors for protein detection

2017 ◽  
Vol 5 (27) ◽  
pp. 5362-5372 ◽  
Author(s):  
Hong-Lei Shuai ◽  
Xu Wu ◽  
Ke-Jing Huang
Keyword(s):  
Molybdenum Disulfide ◽  
Protein Detection ◽  
Electrochemical Aptasensor ◽  
Sandwich Type

In this work, we report the development of an ultrasensitive sandwich-type electrochemical aptasensor for protein detection.

Electrochemical Aptasensor Based on Proximity-Dependent Surface Hybridization Assay for Protein Detection

2009 ◽  
Vol 21 (11) ◽  
pp. 1327-1333 ◽  
Author(s):  
Yan-Li Zhang ◽  
Peng-Fei Pang ◽  
Jian-Hui Jiang ◽  
Guo-Li Shen ◽  
Ru-Qin Yu
Keyword(s):  
Protein Detection ◽  
Hybridization Assay ◽  
Electrochemical Aptasensor

scholarly journals Electrochemical aptasensor for lung cancer-related protein detection in crude blood plasma samples

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Galina S. Zamay ◽  
Tatiana N. Zamay ◽  
Vasilii A. Kolovskii ◽  
Alexandr V. Shabanov ◽  
Yury E. Glazyrin ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Blood Plasma ◽  
Protein Detection ◽  
Plasma Samples ◽  
Electrochemical Aptasensor ◽  
Related Protein

NH2-Ni-MOF electrocatalysts with tunable size/morphology for ultrasensitive C-reactive protein detection via an aptamer binding induced DNA walker–antibody sandwich assay

2018 ◽  
Vol 6 (16) ◽  
pp. 2426-2431 ◽  
Author(s):  
Zhen Wang ◽  
Pei Dong ◽  
Zhongxiong Sun ◽  
Ce Sun ◽  
Huaiyu Bu ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Protein Detection ◽  
Electrochemical Aptasensor ◽  
C Reactive Protein ◽  
Sandwich Assay ◽  
Reactive Protein ◽  
Dna Walker

On the basis of the excellent catalytic activity of NH2-Ni-MOF(c), a sensitive electrochemical aptasensor for CRP detection is first established.

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