Specific detection of live Escherichia coli O157:H7 using tetracysteine-tagged PP01 bacteriophage

2016 ◽  
Vol 86 ◽  
pp. 102-108 ◽  
Author(s):  
Lina Wu ◽  
Yiyi Song ◽  
Tian Luan ◽  
Ling Ma ◽  
Liuqin Su ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Escherichia Coli O157 ◽  
Specific Detection

scholarly journals Simultaneous Detection of Salmonella Strains and Escherichia coli O157:H7 with Fluorogenic PCR and Single-Enrichment-Broth Culture

2000 ◽  
Vol 66 (12) ◽  
pp. 5472-5476 ◽  
Author(s):  
Vijay K. Sharma ◽  
Steve A. Carlson
Keyword(s):  
Escherichia Coli ◽  
Simultaneous Detection ◽  
Broth Culture ◽  
Escherichia Coli O157 ◽  
Specific Detection ◽  
Pcr Assay ◽  
Enrichment Broth ◽  
E Coli ◽  
Serovar Typhimurium ◽  
Low Levels

ABSTRACT A multiplex fluorogenic PCR assay for simultaneous detection of pathogenic Salmonella strains and Escherichia coli O157:H7 was developed and evaluated for use in detecting very low levels of these pathogens in meat and feces. Two sets of primers were used to amplify a junctional segment of virulence genessipB and sipC of Salmonella and an intragenic segment of gene eae of E. coliO157:H7. Fluorogenic reporter probes were included in the PCR assay for automated and specific detection of amplified products. The assay could detect <10 CFU of Salmonella enterica serovar Typhimurium or E. coli O157:H7 per g of meat or feces artificially inoculated with these pathogens and cultured for 6 to 18 h in a single enrichment broth. Detection of amplification products could be completed in ≤4 h after enrichment.

Multiplex PCR for detection of Escherichia coli O157:H7 in foods

Biologia ◽  
2011 ◽  
Vol 66 (3) ◽  
Author(s):  
Barbora Vidová ◽  
Eva Tóthová ◽  
Ľuboslav Blahut ◽  
Viera Horváthová ◽  
Andrej Godány
Keyword(s):  
Escherichia Coli ◽  
Detection Method ◽  
Sample Volume ◽  
Detection Sensitivity ◽  
Escherichia Coli O157 ◽  
Specific Detection ◽  
E Coli ◽  
Colony Forming Units ◽  
Polymerase Chain ◽  
Meat Samples

AbstractEscherichia coli O157:H7 is well known enterohemorrhagic pathogen responsible for infections among animals including a man. The main source of this bacterium is cattle, that is mostly asymptomatic and through that E. coli O157:H7 can simple transfer to food products. Therefore, there is a need for rapid, sensitive and specific detection method. The present work is focused on its detection by a heptaplex polymerase chain reaction, which targets genes from known virulent regions of E. coli O157:H7. According to obtained results this approach is able to reach the detection sensitivity of 4 colony-forming units (CFU) from a culture and 6 and 8 CFU from milk and meat samples, respectively, independently of tested sample volume.

Bedeutung von Escherichia coli O157 beim Schlachtschaf in der Schweiz

2006 ◽  
Vol 148 (6) ◽  
pp. 289-295 ◽  
Author(s):  
C. Zweifel ◽  
M. Kaufmann ◽  
J. Blanco ◽  
R. Stephan
Keyword(s):  
Escherichia Coli ◽  
Escherichia Coli O157

Molecular profiling and antimicrobial susceptibility of Escherichia coli O157:H7 isolated in Bulgaria

2020 ◽  
Vol 23 (3) ◽  
pp. 310-318
Author(s):  
K. Koev ◽  
T. Stoyanchev ◽  
G. Zhelev ◽  
P. Marutsov ◽  
K. Gospodinova ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Escherichia Coli O157 ◽  
Acid Sensitivity ◽  
Lower Extent ◽  
Phenotype Analysis ◽  
Anal Swab ◽  
Amoxicillin Clavulanic Acid ◽  
Primary Identification ◽  
Stx1 Gene ◽  
Cattle Farms

The purpose of this study was to detect the presence of shiga-toxin producing Escherichia coli (STEC) in faeces of healthy dairy cattle and to determine the sensitivity of isolates to several anti­microbial drugs. A total of 1,104 anal swab samples originating from 28 cattle farms were examined. After the primary identification, 30 strains were found to belong to serogroup О157. By means of conventional multiplex PCR, isolates were screened for presence of resistance genes stx1, stx2 and eaeА. Twenty-nine strains possesses amplicons with a size corresponding to genes stx2 and eaeA, one had amplicons also for the stx1 gene and one lacked amplicons of all three genes. Twenty-eight strains demonstrated amplicons equivalent to gene H7. The results from phenotype analysis of resistance showed preserved sensitivity to ceftriaxone, ceftazidime, cefotaxime, cephalothin, streptomycin, gentamicin, tetracycline, enrofloxacin and combinations sulfamethoxazole/trimethoprim and amoxicillin/clavulanic acid. Sensitivity to ampicillin was relatively preserved, although at a lower extent.

Sign in / Sign up

Export Citation Format

Share Document