Integrating fluorescent molecularly imprinted polymer (MIP) sensor particles with a modular microfluidic platform for nanomolar small-molecule detection directly in aqueous samples

There is a growing interest in the technological transfer of highly performing electrochemical sensors within portable analytical devices for the in situ monitoring of environmental contaminants, such as perfluorooctanesulfonic acid (PFOS). In the redesign of biomimetic sensors, many parameters should be taken into account from the working conditions to the electrode surface roughness. A complete characterization of the surface modifiers can help to avoid time-consuming optimizations and better interpret the sensor responses. In the present study, a molecularly imprinted polymer electrochemical sensor (MIP) for PFOS optimized on gold disk electrodes was redesigned on commercial gold screen-printed electrodes. However, its performance investigated by differential pulse voltammetry was found to be poor. Before proceeding with further optimization, a morphological study of the bare and modified electrode surfaces was carried out by scanning electron microscopy–energy-dispersive X-ray spectrometry (SEM–EDS), atomic force microscopy (AFM) and profilometry revealing an heterogeneous distribution of the polymer strongly influenced by the electrode roughness. The high content of fluorine of the target-template molecule allowed to map the distribution of the molecularly imprinted polymer before the template removal and to define a characterization protocol. This case study shows the importance of a multi-analytical characterization approach and identify significant parameters to be considered in similar redesigning studies.

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Determination of ibuprofen, naproxen and diclofenac in aqueous samples using a multi-template molecularly imprinted polymer as selective adsorbent for solid-phase extraction

Journal of Pharmaceutical and Biomedical Analysis ◽

10.1016/j.jpba.2016.05.037 ◽

2016 ◽

Vol 128 ◽

pp. 210-215 ◽

Cited By ~ 60

Author(s):

Lawrence Mzukisi Madikizela ◽

Luke Chimuka

Keyword(s):

Solid Phase Extraction ◽

Molecularly Imprinted Polymer ◽

Solid Phase ◽

Phase Extraction ◽

Aqueous Samples ◽

Imprinted Polymer ◽

Molecularly Imprinted ◽

Selective Adsorbent

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Optimization of the class-selective extraction of triazines from aqueous samples using a molecularly imprinted polymer by a comprehensive approach of the retention mechanism

Journal of Chromatography A ◽

10.1016/s0021-9673(03)00552-1 ◽

2003 ◽

Vol 999 (1-2) ◽

pp. 23-33 ◽

Cited By ~ 87

Author(s):

F Chapuis ◽

V Pichon ◽

F Lanza ◽

S Sellergren ◽

M.-C Hennion

Keyword(s):

Molecularly Imprinted Polymer ◽

Selective Extraction ◽

Retention Mechanism ◽

Comprehensive Approach ◽

Aqueous Samples ◽

Imprinted Polymer ◽

Molecularly Imprinted

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Electrochemical Sensor Based on Molecularly Imprinted Polymer for the Detection of Cefalexin

Biosensors ◽

10.3390/bios9010031 ◽

2019 ◽

Vol 9 (1) ◽

pp. 31 ◽

Cited By ~ 11

Author(s):

Bogdan Feier ◽

Adrian Blidar ◽

Alexandra Pusta ◽

Paula Carciuc ◽

Cecilia Cristea

Keyword(s):

Electrochemical Sensor ◽

Molecularly Imprinted Polymer ◽

Electrochemical Impedance ◽

Limit Of Detection ◽

Imprinted Polymer ◽

Boron Doped Diamond ◽

Molecularly Imprinted ◽

Diamond Electrode ◽

The Difference ◽

Mip Sensor

In this study, a new electrochemical sensor was developed for the detection of cefalexin (CFX), based on the use of a molecularly imprinted polymer (MIP) obtained by electro‒polymerization in an aqueous medium of indole-3-acetic acid (I3AA) on a glassy carbon electrode (GCE) and on boron-doped diamond electrode (BDDE). The two different electrodes were used in order to assess how their structural differences and the difference in the potential applied during electrogeneration of the MIP translate to the performances of the MIP sensor. The quantification of CFX was performed by using the electrochemical signal of a redox probe before and after the rebinding of the template. The modified electrode was characterized using atomic force microscopy (AFM), scanning electron microscopy (SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The influence of different parameters on the fabrication of the sensor was tested, and the optimized method presented high selectivity and sensitivity. The MIP-based electrode presented a linear response for CFX concentration range of 10 to 1000 nM, and a limit of detection of 3.2 nM and 4.9 nM was obtained for the BDDE and the GCE, respectively. The activity of the sensor was successfully tested in the presence of some other cephalosporins and of other pharmaceutical compounds. The developed method was successfully applied to the detection of cefalexin from real environmental and pharmaceutical samples.

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