Development of an aptamer-based field effect transistor biosensor for quantitative detection of Plasmodium falciparum glutamate dehydrogenase in serum samples

2019 ◽  
Vol 123 ◽  
pp. 30-35 ◽  
Author(s):  
Naveen K. Singh ◽  
Phurpa Dema Thungon ◽  
Pedro Estrela ◽  
Pranab Goswami
2007 ◽  
Vol 111 (24) ◽  
pp. 8667-8670 ◽  
Author(s):  
Masuhiro Abe ◽  
Katsuyuki Murata ◽  
Atsuhiko Kojima ◽  
Yasuo Ifuku ◽  
Mitsuaki Shimizu ◽  
...  

2021 ◽  
Author(s):  
Merve Oğuz ◽  
İpek Avci ◽  
Mustafa SEN

Here, an extended – gate field effect transistor (EGFET) urea microsensor based on modified polypyrrole (PPy) is reported for the quantitative detection of urea in aqueous solution. the EGFET urea sensor was made by integrating a small and cheap metal oxide semiconductor field - effect transistor (MOSFET) with a Au microelectrode modified with urease and pH sensitive PPy. First, the urease was added to a pyrrole solution and then pyrrole/urease solution was electropolymerized on the surface of gold microelectrode in galvostanic mode to produce a urea sensitive microelectrode. The microsensor was imaged using a stereo microscope to confirm the polymerization of pyrrole/urease. The EGFET urea microsensor was tested in deionized water containing various concentrations of urea. The electrode showed a linear response for a wide concentration range of urea from 10−9 to 10−5 M with a sensitivity of 35.5 mV/decade urea.


Sensor Review ◽  
2018 ◽  
Vol 38 (2) ◽  
pp. 181-193 ◽  
Author(s):  
Saeid Masoumi ◽  
Hassan Hajghassem ◽  
Alireza Erfanian ◽  
Ahmad Molaei Rad

Purpose Smart sensors based on graphene field effect transistor (GFET) and biological receptors are regarded as a promising nanomaterial that could be the basis for future generation of low-power, faster, selective real-time monitoring of target analytes and smaller electronics. So, the purpose of this paper is to provide details of sensors based on selective nanocoatings by combining trinitrotoluene (TNT) receptors (Trp-His-Trp) bound to conjugated polydiacetylene polymers on a graphene channel in GFET for detecting explosives TNT. Design/methodology/approach Following an introduction, this paper describes the way of manufacturing of the GFET sensor by using investigation methods for transferring graphene sheet from Cu foil to target substrates, which is functionalized by the TNT peptide receptors, to offer a system which has the capability of answering the presence of related target molecules (TNT). Finally, brief conclusions are drawn. Findings In a word, shortly after graphene discovery, it has been explored with a variety of methods gradually. Because of its exceptional electrical properties (e.g. extremely high carrier mobility and capacity), electrochemical properties such as high electron transfer rate and structural properties, graphene has already showed great potential and success in chemical and biological sensing fields. Therefore, the authors used a biological receptor with a field effect transistor (FET) based on graphene to fabricate sensor for achieving high sensitivity and selectivity that can detect explosive substances such as TNT. The transport property changed compared to that of the FET made by intrinsic graphene, that is, the Dirac point position moved from positive Vg to negative Vg, indicating the transition of graphene from p-type to n-type after annealing in TNT, and the results show the bipolar property change of GFET with the TNT concentration and the possibility to develop a robust, easy-to-use and low-cost TNT detection method for performing a sensitive, reliable and semi-quantitative detection in a wide detection range. Originality/value In this timeframe of history, TNT is a common explosive used in both military and industrial settings. Its convenient handling properties and explosive strength make it a common choice in military operations and bioterrorism. TNT and other conventional explosives are the mainstays of terrorist bombs and the anti-personnel mines that kill or injure more than 15,000 people annually in war-torn countries. In large, open-air environments, such as airports, train stations and minefields, concentrations of these explosives can be vanishingly small – a few parts of TNT, for instance, per trillion parts of air. That can make it impossible for conventional bomb and mine detectors to detect the explosives and save lives. So, in this paper, the authors report a potential solution with design and manufacture of a GFET sensor based on a biological receptor for real-time detection of TNT explosives specifically.


2D Materials ◽  
2021 ◽  
Author(s):  
Junqing Wei ◽  
Zhihan Zhao ◽  
Fengting Luo ◽  
Kuibo Lan ◽  
Ruibing Chen ◽  
...  

Abstract Recently, the coronavirus disease (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread globally with major impact on public health. Novel methods that enable fast and efficient detection of the virus and the associated biomarkers, such as SARS-CoV-2 antibodies, may provide alterative opportunities for early diagnosis, disease status monitoring, and the development of vaccines. Here, we report the fabrication of a functionalized MoS2-field effect transistor (FET) for sensitive and quantitative detection of antibodies against SARS-CoV-2 spike protein receptor binding domain (S-RBD) in vaccinated serum specimens. The device was modified by SARS-CoV-2 S-RBD fusion protein on the surface and enabled rapid detection of SARS-CoV-2 antibodies. In addition, an on-chip calibration method was established for quantitative measurement. Furthermore, this method was applied to measure the levels of S-RBD antibodies in serum specimens from vaccinated donors. The devices showed no response to negative control samples from individuals who didn’t receive vaccination, suggesting the high specificity of this method. This study illustrated the successful fabrication of S-RBD functionalized MoS2-FET with potential clinical applications to facilitate vaccine development and efficacy evaluation.


2010 ◽  
Vol E93-C (5) ◽  
pp. 540-545 ◽  
Author(s):  
Dong Seup LEE ◽  
Hong-Seon YANG ◽  
Kwon-Chil KANG ◽  
Joung-Eob LEE ◽  
Jung Han LEE ◽  
...  

2014 ◽  
Vol E97.C (7) ◽  
pp. 677-682
Author(s):  
Sung YUN WOO ◽  
Young JUN YOON ◽  
Jae HWA SEO ◽  
Gwan MIN YOO ◽  
Seongjae CHO ◽  
...  

2019 ◽  
Vol 24 (4) ◽  
pp. 407-414
Author(s):  
Oksana V. Gubanova ◽  
◽  
Evgeniy V. Kuznetsov ◽  
Elena N. Rybachek ◽  
Alexander N. Saurov ◽  
...  

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