A self-powered photoelectrochemical biosensor for H2O2, and xanthine oxidase activity based on enhanced chemiluminescence resonance energy transfer through slow light effect in inverse opal TiO2

2019 ◽  
Vol 141 ◽  
pp. 111385 ◽  
Author(s):  
Bekir Çakıroğlu ◽  
Mahmut Özacar
Keyword(s):  
Energy Transfer ◽  
Oxidase Activity ◽  
Slow Light ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Inverse Opal ◽  
Light Effect ◽  
Enhanced Chemiluminescence ◽  
Photoelectrochemical Biosensor ◽  
Self Powered

Quantum Dot Bioconjugates as Energy Donors in Fluorescence Resonance Energy Transfer Assays

2003 ◽  
Vol 773 ◽  
Author(s):  
Aaron R. Clapp ◽  
Igor L. Medintz ◽  
J. Matthew Mauro ◽  
Hedi Mattoussi
Keyword(s):  
Energy Transfer ◽  
Quantum Dot ◽  
Organic Dyes ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Genetically Engineered ◽  
Molar Ratio ◽  
Binding Assays ◽  
Specific Sequence ◽  
Donor Acceptor

AbstractLuminescent CdSe-ZnS core-shell quantum dot (QD) bioconjugates were used as energy donors in fluorescent resonance energy transfer (FRET) binding assays. The QDs were coated with saturating amounts of genetically engineered maltose binding protein (MBP) using a noncovalent immobilization process, and Cy3 organic dyes covalently attached at a specific sequence to MBP were used as energy acceptor molecules. Energy transfer efficiency was measured as a function of the MBP-Cy3/QD molar ratio for two different donor fluorescence emissions (different QD core sizes). Apparent donor-acceptor distances were determined from these FRET studies, and the measured distances are consistent with QD-protein conjugate dimensions previously determined from structural studies.

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