scholarly journals Enhanced triacylglycerol accumulation in open cultivation of microalgae using an air self-sufficient aerator

2021 ◽  
pp. 100916
Author(s):  
Kazuhiro Itoh ◽  
Shogo Taguchi ◽  
Norifumi Yoshida ◽  
Takuji Yamamoto ◽  
Kouji Maeda
Keyword(s):  
Triacylglycerol Accumulation

scholarly journals The SUGAR-DEPENDENT1 Lipase Limits Triacylglycerol Accumulation in Vegetative Tissues of Arabidopsis

2013 ◽  
Vol 162 (3) ◽  
pp. 1282-1289 ◽  
Author(s):  
A. A. Kelly ◽  
H. van Erp ◽  
A.-L. Quettier ◽  
E. Shaw ◽  
G. Menard ◽  
...  
Keyword(s):  
Vegetative Tissues ◽  
Triacylglycerol Accumulation

scholarly journals AP2/ERF and R2R3-MYB family transcription factors: potential associations between temperature stress and lipid metabolism in Auxenochlorella protothecoides

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Guanlan Xing ◽  
Jinyu Li ◽  
Wenli Li ◽  
Sin Man Lam ◽  
Hongli Yuan ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Lipid Metabolism ◽  
Temperature Stress ◽  
Expression Profiles ◽  
Critical Factor ◽  
Biofuel Production ◽  
Systematic Analysis ◽  
Triacylglycerol Accumulation ◽  
Response To Temperature ◽  
R2r3 Myb

Abstract Background Both APETALA2/Ethylene Responsive Factor (AP2/ERF) superfamily and R2R3-MYB family were from one of the largest diverse families of transcription factors (TFs) in plants, and played important roles in plant development and responses to various stresses. However, no systematic analysis of these TFs had been conducted in the green algae A. protothecoides heretofore. Temperature was a critical factor affecting growth and lipid metabolism of A. protothecoides. It also remained largely unknown whether these TFs would respond to temperature stress and be involved in controlling lipid metabolism process. Results Hereby, a total of six AP2 TFs, six ERF TFs and six R2R3-MYB TFs were identified and their expression profiles were also analyzed under low-temperature (LT) and high-temperature (HT) stresses. Meanwhile, differential adjustments of lipid pathways were triggered, with enhanced triacylglycerol accumulation. A co-expression network was built between these 18 TFs and 32 lipid-metabolism-related genes, suggesting intrinsic associations between TFs and the regulatory mechanism of lipid metabolism. Conclusions This study represented an important first step towards identifying functions and roles of AP2 superfamily and R2R3-MYB family in lipid adjustments and response to temperature stress. These findings would facilitate the biotechnological development in microalgae-based biofuel production and the better understanding of photosynthetic organisms’ adaptive mechanism to temperature stress.

scholarly journals Analysis of triacylglycerol accumulation under nitrogen deprivation in the red alga Cyanidioschyzon merolae

Microbiology ◽  
2016 ◽  
Vol 162 (5) ◽  
pp. 803-812 ◽  
Author(s):  
Masakazu Toyoshima ◽  
Natsumi Mori ◽  
Takashi Moriyama ◽  
Osami Misumi ◽  
Naoki Sato
Keyword(s):  
Red Alga ◽  
Cyanidioschyzon Merolae ◽  
Nitrogen Deprivation ◽  
Triacylglycerol Accumulation

scholarly journals Investigating mixotrophic metabolism in the model diatom Phaeodactylum tricornutum

2017 ◽  
Vol 372 (1728) ◽  
pp. 20160404 ◽  
Author(s):  
Valeria Villanova ◽  
Antonio Emidio Fortunato ◽  
Dipali Singh ◽  
Davide Dal Bo ◽  
Melissa Conte ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Phaeodactylum Tricornutum ◽  
Photosynthetic Activity ◽  
Biomass Accumulation ◽  
Point Of View ◽  
Physiological Data ◽  
Pennate Diatom ◽  
Metabolic Modelling ◽  
Central Carbon ◽  
Triacylglycerol Accumulation

Diatoms are prominent marine microalgae, interesting not only from an ecological point of view, but also for their possible use in biotechnology applications. They can be cultivated in phototrophic conditions, using sunlight as the sole energy source. Some diatoms, however, can also grow in a mixotrophic mode, wherein both light and external reduced carbon contribute to biomass accumulation. In this study, we investigated the consequences of mixotrophy on the growth and metabolism of the pennate diatom Phaeodactylum tricornutum , using glycerol as the source of reduced carbon. Transcriptomics, metabolomics, metabolic modelling and physiological data combine to indicate that glycerol affects the central-carbon, carbon-storage and lipid metabolism of the diatom. In particular, provision of glycerol mimics typical responses of nitrogen limitation on lipid metabolism at the level of triacylglycerol accumulation and fatty acid composition. The presence of glycerol, despite provoking features reminiscent of nutrient limitation, neither diminishes photosynthetic activity nor cell growth, revealing essential aspects of the metabolic flexibility of these microalgae and suggesting possible biotechnological applications of mixotrophy. This article is part of the themed issue ‘The peculiar carbon metabolism in diatoms'.

scholarly journals Effects of milk diets containing beef tallow or coconut oil on the fatty acid metabolism of liver slices from preruminant calves

2000 ◽  
Vol 84 (3) ◽  
pp. 309-318 ◽  
Author(s):  
Benoît Graulet ◽  
Dominique Gruffat-Mouty ◽  
Denys Durand ◽  
Dominique Bauchart
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Oxidation ◽  
Beef Tallow ◽  
Neutral Lipids ◽  
Lipid Production ◽  
Coconut Oil ◽  
Acid Oxidation ◽  
Milk Diet ◽  
Liver Slices ◽  
Triacylglycerol Accumulation

Coconut oil (CO) induces a triacylglycerol infiltration in the hepatocytes of preruminant calves when given as the sole source of fat in the milk diet over a long-term period. Metabolic pathways potentially involved in this hepatic triacylglycerol accumulation were studied by in vitro methods on liver slices from preruminant Holstein × Friesian male calves fed a conventional milk diet containing CO (n 5) or beef tallow (BT, n 5) for 19 d. Liver slices were incubated for 12 h in the presence of 0·8 mM-[14C] oleate or -[14C] laurate added to the medium. Fatty acid oxidation was determined by measuring the production of CO2 (total oxidation) and acid-soluble products (partial oxidation). Production of CO2 was 1·7–3·6-fold lower (P 0·0490) and production of acid-soluble products tended to be lower (P = 0·0625) in liver slices of CO- than BT-fed calves. Fatty acid esterification as neutral lipids was 2·6– to 3·1–fold higher (P = 0·0088) in liver slices prepared from calves fed the CO diet compared with calves fed the BT diet. By contrast with what occurs in the liver of rats fed CO, the increase in neutral lipid production did not stimulate VLDL secretion by the hepatocytes of calves fed with CO, leading to a triacylglycerol accumulation in the cytosol. It could be explained by the reduction of fatty acid oxidation favouring esterification in the form of triacylglycerols, in association with a limited availability of triacylglycerols and/or apolipoprotein B for VLDL packaging and subsequent secretion.

scholarly journals An Acyl-CoA Synthetase in Mycobacterium tuberculosis Involved in Triacylglycerol Accumulation during Dormancy

PLoS ONE ◽  
2014 ◽  
Vol 9 (12) ◽  
pp. e114877 ◽  
Author(s):  
Jaiyanth Daniel ◽  
Tatiana Sirakova ◽  
Pappachan Kolattukudy
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Triacylglycerol Accumulation

scholarly journals The Role of p38 MAPK in Triacylglycerol Accumulation During Apoptosis

PROTEOMICS ◽  
2019 ◽  
pp. 1900160 ◽  
Author(s):  
Nasi Li ◽  
Marie Saitou ◽  
G. Ekin Atilla‐Gokcumen
Keyword(s):  
P38 Mapk ◽  
Triacylglycerol Accumulation

scholarly journals RICE ACYL-COA-BINDING PROTEIN6 Affects Acyl-CoA Homeostasis and Growth in Rice

Rice ◽  
2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Wei Meng ◽  
Lijian Xu ◽  
Zhi-Yan Du ◽  
Fang Wang ◽  
Rui Zhang ◽  
...  
Keyword(s):  
Leaf Growth ◽  
Defense Responses ◽  
Ros Homeostasis ◽  
Similar Phenotype ◽  
Jasmonic Acid Level ◽  
Triacylglycerol Accumulation ◽  
Acyl Coenzyme A ◽  
Regulatory Properties ◽  
Comparative Transcriptomic Analysis

Abstract Backgrounds Acyl-coenzyme A (CoA) esters are important intermediates in lipid metabolism with regulatory properties. Acyl-CoA-binding proteins bind and transport acyl-CoAs to fulfill these functions. RICE ACYL-COA-BINDING PROTEIN6 (OsACBP6) is currently the only one peroxisome-localized plant ACBP that has been proposed to be involved in β-oxidation in transgenic Arabidopsis. The role of the peroxisomal ACBP (OsACBP6) in rice (Oryza sativa) was investigated. Results Here, we report on the function of OsACBP6 in rice. The osacbp6 mutant showed diminished growth with reduction in root meristem activity and leaf growth. Acyl-CoA profiling and lipidomic analysis revealed an increase in acyl-CoA content and a slight triacylglycerol accumulation caused by the loss of OsACBP6. Comparative transcriptomic analysis discerned the biological processes arising from the loss of OsACBP6. Reduced response to oxidative stress was represented by a decline in gene expression of a group of peroxidases and peroxidase activities. An elevation in hydrogen peroxide was observed in both roots and shoots/leaves of osacbp6. Taken together, loss of OsACBP6 not only resulted in a disruption of the acyl-CoA homeostasis but also peroxidase-dependent reactive oxygen species (ROS) homeostasis. In contrast, osacbp6-complemented transgenic rice displayed similar phenotype to the wild type rice, supporting a role for OsACBP6 in the maintenance of the acyl-CoA pool and ROS homeostasis. Furthermore, quantification of plant hormones supported the findings observed in the transcriptome and an increase in jasmonic acid level occurred in osacbp6. Conclusions In summary, OsACBP6 appears to be required for the efficient utilization of acyl-CoAs. Disruption of OsACBP6 compromises growth and led to provoked defense response, suggesting a correlation of enhanced acyl-CoAs content with defense responses.

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