Membrane shape as determinant of protein properties

2021 ◽  
Vol 273 ◽  
pp. 106587
Author(s):  
José Carlos Bozelli ◽  
Sukhvershjit S. Aulakh ◽  
Richard M. Epand
2021 ◽  
Vol 11 (9) ◽  
pp. 3846
Author(s):  
Andrea Montanino ◽  
Carlo Olivieri ◽  
Giulio Zuccaro ◽  
Maurizio Angelillo

The assessment of the equilibrium and the safety of masonry vaults is of high relevance for the conservation and restoration of historical heritage. In the literature many approaches have been proposed for this tasks, starting from the 17th century. In this work we focus on the Membrane Equilibrium Analysis, developed under the Heyman’s theory of Limit Analysis. Within this theory, the equilibrium of a vault is assessed if it is possible to find at least one membrane surface, between the volume of the vaults, being in equilibrium under the given loads through a purely compressive stress field. The equilibrium of membranes is described by a second order partial differential equation, which is definitely elliptic only when a negative semidefinite stress is assigned, and the shape is the unknown of the problem. The proposed algorithm aims at finding membrane shapes, entirely comprised between the geometry of the vault, in equilibrium with admissible stress fields, through the minimization of an error function with respect to shape parameters of the stress potential, and then, with respect to the boundary values of the membrane shape. The application to two test cases shows the viability of this tool for the assessment of the equilibrium of existing masonry vaults.


Separations ◽  
2021 ◽  
Vol 8 (4) ◽  
pp. 53
Author(s):  
Clarissa Braccia ◽  
Bhakti Prinsi ◽  
Mara Colzani ◽  
Alessandra A. Altomare ◽  
Luca Espen ◽  
...  

The benefits of ginseng have been mainly attributed to its triterpenoids, called ginsenosides. Recent genome sequencing of the Panax ginseng has paved the way for in-depth proteomic studies of this medicinal plant. The current study was conducted to deepen the proteomic information on the root proteome of Korean ginseng. Proteomic workflow was optimized by testing two different strategies, characterized by the phenol extraction procedure, the presence or the absence of SDS-PAGE fractionation step, and nano-scale liquid chromatographic tandem mass spectrometry (nLC-MS/MS) analysis. The results highlighted an evident improvement of proteome extraction by the combination of phenol extraction with SDS-PAGE before the nLC-MS/MS analysis. In addition, a dramatic impact of the steaming process (the treatment to produce red ginseng from ginseng) on protein properties was observed. Overall, the analyses of Korean ginseng permitted the characterization of a total of 2412 proteins. A large number of identified proteins belonged to the functional categories of protein and carbon/energy metabolism (22.4% and 14.6%, respectively). The primary and secondary metabolisms are major metabolic pathways, which emerged from the proteomic analysis. In addition, a large number of proteins known to play an important role in response to (a)biotic stresses were also identified. The current proteomic study not only confirmed the previous transcriptomic and proteomic reports but also extended proteomic information, including the main metabolic pathways involved in Korean ginseng.


2009 ◽  
Vol 24 (4) ◽  
pp. 1095-1104 ◽  
Author(s):  
Atsushi Kurotani ◽  
Tetsuo Takagi ◽  
Mitsutoshi Toyama ◽  
Mikako Shirouzu ◽  
Shigeyuki Yokoyama ◽  
...  

1981 ◽  
Vol 101 (12) ◽  
pp. 1129-1136 ◽  
Author(s):  
TAKASHI SATO ◽  
MASAKO IKEDA ◽  
YASUNORI KANAHO ◽  
TATSUZO FUJII

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