scholarly journals Energy Taxis in Escherichia Coli on the Single-Cell Level

2017 ◽  
Vol 112 (3) ◽  
pp. 277a
Author(s):  
Tatyana Perlova ◽  
Martin Gruebele ◽  
Yann R. Chemla
Keyword(s):  
Escherichia Coli ◽  
Single Cell ◽  
Single Cell Level ◽  
Cell Level ◽  
Energy Taxis

scholarly journals AES and ToF-SIMS combination for single cell chemical imaging of gold nanoparticle-labeled Escherichia coli

2021 ◽  
Author(s):  
Cecile COURREGES ◽  
Mélanie Bonnecaze ◽  
Delphine Flahaut ◽  
Sophie Nolivos ◽  
Regis Grimaud ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gold Nanoparticles ◽  
Single Cell ◽  
Electron Spectroscopy ◽  
Auger Electron ◽  
Chemical Imaging ◽  
Single Cell Level ◽  
Cell Level ◽  
Tof Sims ◽  
Escherichia Coli Cells

A chemical fingerprint of Escherichia coli cells surface labeled by gelatin coated gold nanoparticles was obtained by combining Auger Electron Spectroscopy (AES) for single cell level chemical images, and Time-of-Flight...

scholarly journals High-Frequency Phage-Mediated Gene Transfer among Escherichia coli Cells, Determined at the Single-Cell Level

2007 ◽  
Vol 73 (10) ◽  
pp. 3291-3299 ◽  
Author(s):  
Takehiko Kenzaka ◽  
Katsuji Tani ◽  
Akiko Sakotani ◽  
Nobuyasu Yamaguchi ◽  
Masao Nasu
Keyword(s):  
Escherichia Coli ◽  
Gene Transfer ◽  
Single Cell ◽  
Dna Sequences ◽  
High Frequency ◽  
Selective Medium ◽  
Single Cell Level ◽  
Cell Level ◽  
Whole Genome Analysis

ABSTRACT Recent whole-genome analysis suggests that lateral gene transfer by bacteriophages has contributed significantly to the genetic diversity of bacteria. To accurately determine the frequency of phage-mediated gene transfer, we employed cycling primed in situ amplification-fluorescent in situ hybridization (CPRINS-FISH) and investigated the movement of the ampicillin resistance gene among Escherichia coli cells mediated by phage at the single-cell level. Phages P1 and T4 and the newly isolated E. coli phage EC10 were used as vectors. The transduction frequencies determined by conventional plating were 3 × 10−8 to 2 × 10−6, 1 × 10−8 to 4 × 10−8, and <4 × 10−9 to 4 × 10−8 per PFU for phages P1, T4, and EC10, respectively. The frequencies of DNA transfer determined by CPRINS-FISH were 7 × 10−4 to 1 × 10−3, 9 × 10−4 to 3 × 10−3, and 5 × 10−4 to 4 × 10−3 for phages P1, T4, and EC10, respectively. Direct viable counting combined with CPRINS-FISH revealed that more than 20% of the cells carrying the transferred gene retained their viabilities. These results revealed that the difference in the number of viable cells carrying the transferred gene and the number of cells capable of growth on the selective medium was 3 to 4 orders of magnitude, indicating that phage-mediated exchange of DNA sequences among bacteria occurs with unexpectedly high frequency.

scholarly journals Long-term growth data of Escherichia coli at a single-cell level

2017 ◽  
Vol 4 (1) ◽  
Author(s):  
Yu Tanouchi ◽  
Anand Pai ◽  
Heungwon Park ◽  
Shuqiang Huang ◽  
Nicolas E. Buchler ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Single Cell ◽  
Single Cell Level ◽  
Growth Data ◽  
Cell Level

scholarly journals Dynamics of Solitary Predation by Myxococcus xanthus on Escherichia coli Observed at the Single-Cell Level

2019 ◽  
Vol 86 (3) ◽  
Author(s):  
Wenchao Zhang ◽  
Yan Wang ◽  
Huining Lu ◽  
Qin Liu ◽  
Chuandong Wang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Single Cell ◽  
Myxococcus Xanthus ◽  
Predatory Behavior ◽  
Single Cell Level ◽  
Cell Level ◽  
Prey Cell ◽  
Content Type ◽  
E Coli ◽  
Tracking Technique

ABSTRACT The predatory behavior of Myxococcus xanthus has attracted extensive attention due to its unique social traits and inherent biological activities. In addition to group hunting, individual M. xanthus cells are able to kill and lyse prey cells; however, there is little understanding of the dynamics of solitary predation. In this study, by employing a bacterial tracking technique, we investigated M. xanthus predatory dynamics on Escherichia coli at the single-cell level. The killing and lysis of E. coli by a single M. xanthus cell was monitored in real time by microscopic observation, and the plasmolysis of prey cells was identified at a relatively early stage of solitary predation. After quantitative characterization of their solitary predatory behavior, M. xanthus cells were found to respond more dramatically to direct contact with live E. coli cells than heat-killed or UV-killed cells, showing slower predator motion and faster lysing of prey. Among the three contact-dependent killing modes classified according to the major subareas of M. xanthus cells in contact with prey, leading pole contact was observed most. After killing the prey, approximately 72% of M. xanthus cells were found to leave without thorough degradation of the lysed prey, and this postresidence behavior is described as a lysis-leave pattern, indicating that solitary predation has low efficiency in terms of prey-cell consumption. Our results provide a detailed description of the single-cell level dynamics of M. xanthus solitary predation from both prey and predator perspectives. IMPORTANCE Bacterial predation plays multiple essential roles in bacterial selection and mortality within microbial ecosystems. In addition to its ecological and evolutionary importance, many potential applications of bacterial predation have been proposed. The myxobacterium Myxococcus xanthus is a well-known predatory member of the soil microbial community. Its predation is commonly considered a collective behavior comparable to a wolf pack attack; however, individual M. xanthus cells are also able to competently lead to the lysis of a prey cell. Using a bacterial tracking technique, we are able to observe and analyze solitary predation by M. xanthus on Escherichia coli at the single-cell level and reveal the dynamics of both predator and prey during the process. The present study will not only provide a comprehensive understanding of M. xanthus solitary predation but also help to explain why M. xanthus often displays multicellular characteristic predatory behaviors in nature, while a single cell is capable of predation.

Single cell level detection of Escherichia coli in microfluidic device

2008 ◽  
Vol 23 (8) ◽  
pp. 1303-1306 ◽  
Author(s):  
Jin-Hee Han ◽  
Brian C. Heinze ◽  
Jeong-Yeol Yoon
Keyword(s):  
Escherichia Coli ◽  
Single Cell ◽  
Microfluidic Device ◽  
Single Cell Level ◽  
Cell Level

scholarly journals Enrichment of persisters enabled by a ß-lactam-induced filamentation method reveals their stochastic single-cell awakening

2019 ◽  
Vol 2 (1) ◽  
Author(s):  
Etthel M. Windels ◽  
Zacchari Ben Meriem ◽  
Taiyeb Zahir ◽  
Kevin J. Verstrepen ◽  
Pascal Hersen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Single Cell ◽  
Low Frequency ◽  
Single Cell Level ◽  
Cell Level ◽  
Enrichment Method ◽  
Bacterial Populations ◽  
Highly Effective ◽  
Lethal Doses

AbstractWhen exposed to lethal doses of antibiotics, bacterial populations are most often not completely eradicated. A small number of phenotypic variants, defined as ‘persisters’, are refractory to antibiotics and survive treatment. Despite their involvement in relapsing infections, processes determining phenotypic switches from and to the persister state largely remain elusive. This is mainly due to the low frequency of persisters and the lack of reliable persistence markers, both hampering studies of persistence at the single-cell level. Here we present a highly effective persister enrichment method involving cephalexin, an antibiotic that induces extensive filamentation of susceptible cells. We used our enrichment method to monitor outgrowth of Escherichia coli persisters at the single-cell level, thereby conclusively demonstrating that persister awakening is a stochastic phenomenon. We anticipate that our approach can have far-reaching consequences in the persistence field, by allowing single-cell studies at a much higher throughput than previously reported.

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