Determination of Ligand Migration Pathways in Human Cytoglobin

The content of trace elements in the soils of the Vecherny Oasis (Enderby Land, East Antarctica), where the construction of the Belarusian Antarctic Station started in December 2015, is considered. The results of the research are based on data collected during four Belarusian Antarctic expeditions in the period from 2011 to 2017, and analytical testing of soil samples taken from impacted and non-impacted sites. A total of 22 soil samples were analyzed for the content of trace elements; to compare the levels of accumulation and possible migration pathways, 7 samples of bottom sediments were also analyzed. Determination of trace elements was carried out using the AAS method (Cd, Cr, Cu, Pb, Ni, Zn, Fe, Mn) and emission spectral analysis (about 40 elements). The average values and range of concentrations of trace elements in soils and bottom sediments of the oasis are presented. The possible dependence of the trace elements content on the location positions in the landscape and on the sources of impact is discussed. It is shown, that the variability of metals content in soil profile for background site is low. In comparison with other oases of Antarctica no hotspots have been revealed and no significant areas of soil contamination have been identified yet, which is largely due to the fragmentation of the soil cover and lack of significant sources of pollution.

Download Full-text

Atomic level computational identification of ligand migration pathways between solvent and binding site in myoglobin

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.0710825105 ◽

2008 ◽

Vol 105 (27) ◽

pp. 9204-9209 ◽

Cited By ~ 112

Author(s):

J. Z. Ruscio ◽

D. Kumar ◽

M. Shukla ◽

M. G. Prisant ◽

T. M. Murali ◽

...

Keyword(s):

Binding Site ◽

Atomic Level ◽

Ligand Migration ◽

Computational Identification ◽

Migration Pathways

Download Full-text

Faculty Opinions recommendation of Atomic level computational identification of ligand migration pathways between solvent and binding site in myoglobin.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1115551.571596 ◽

2008 ◽

Author(s):

Nathan Baker

Keyword(s):

Binding Site ◽

Atomic Level ◽

Ligand Migration ◽

Computational Identification ◽

Migration Pathways

Download Full-text

Tracking ligand-migration pathways of carbonmonoxy myoglobin in crystals at cryogenic temperatures

Acta Crystallographica Section A Foundations of Crystallography ◽

10.1107/s0108767309050752 ◽

2010 ◽

Vol 66 (2) ◽

pp. 220-228 ◽

Cited By ~ 4

Author(s):

Ayana Tomita ◽

Tokushi Sato ◽

Shunsuke Nozawa ◽

Shin-ya Koshihara ◽

Shin-ichi Adachi

Keyword(s):

Structural Changes ◽

Continuous Wave ◽

Cryogenic Temperatures ◽

Amino Acid Residues ◽

Migration Pathway ◽

Laser Pumping ◽

Ligand Migration ◽

Migration Dynamics ◽

Carbonmonoxy Myoglobin ◽

Migration Pathways

In order to explore the ligand-migration dynamics in myoglobin induced by photodissociation, cryogenic X-ray crystallographic investigations of carbonmonoxy myoglobin crystals illuminated by continuous wave and pulsed lasers at 1–15 kHz repetition rate have been carried out. Here it is shown that this novel method, extended pulsed-laser pumping of carbonmonoxy myoglobin, promotes ligand migration in the protein matrix by crossing the glass transition temperature repeatedly, and enables the visualization of the migration pathway of the photodissociated ligands in native Mb at cryogenic temperatures. It has revealed that the migration of the CO molecule into each cavity induces structural changes of the amino-acid residues around the cavity which result in the expansion of the cavity. The sequential motion of the ligand and the cavity suggests a self-opening mechanism of the ligand-migration channel arising by induced fit.

Download Full-text

Galactic orbits of stars

Symposium - International Astronomical Union ◽

10.1017/s0074180900105340 ◽

1966 ◽

Vol 25 ◽

pp. 93-97

Author(s):

Richard Woolley

Keyword(s):

Globular Cluster ◽

Potential Field ◽

High Velocity ◽

Velocity Vector ◽

Variable Stars ◽

The Sun ◽

Proper Motions ◽

Rr Lyrae ◽

The Galaxy

It is now possible to determine proper motions of high-velocity objects in such a way as to obtain with some accuracy the velocity vector relevant to the Sun. If a potential field of the Galaxy is assumed, one can compute an actual orbit. A determination of the velocity of the globular clusterωCentauri has recently been completed at Greenwich, and it is found that the orbit is strongly retrograde in the Galaxy. Similar calculations may be made, though with less certainty, in the case of RR Lyrae variable stars.

Download Full-text

Distance to SN 1987A and the LMC

Symposium - International Astronomical Union ◽

10.1017/s0074180900118959 ◽

1999 ◽

Vol 190 ◽

pp. 549-554

Author(s):

Nino Panagia

Keyword(s):

Angular Size ◽

Large Magellanic Cloud ◽

Magellanic Cloud ◽

Light Curves ◽

Distance Modulus ◽

Absolute Size ◽

Sn 1987A

Using the new reductions of the IUE light curves by Sonneborn et al. (1997) and an extensive set of HST images of SN 1987A we have repeated and improved Panagia et al. (1991) analysis to obtain a better determination of the distance to the supernova. In this way we have derived an absolute size of the ringRabs= (6.23 ± 0.08) x 1017cm and an angular sizeR″ = 808 ± 17 mas, which give a distance to the supernovad(SN1987A) = 51.4 ± 1.2 kpc and a distance modulusm–M(SN1987A) = 18.55 ± 0.05. Allowing for a displacement of SN 1987A position relative to the LMC center, the distance to the barycenter of the Large Magellanic Cloud is also estimated to bed(LMC) = 52.0±1.3 kpc, which corresponds to a distance modulus ofm–M(LMC) = 18.58±0.05.

Download Full-text

International determination of the total motion of the pole

Symposium - International Astronomical Union ◽

10.1017/s0074180900177824 ◽

1961 ◽

Vol 13 ◽

pp. 29-41

Author(s):

Wm. Markowitz

Keyword(s):

Secular Motion ◽

The Future

A symposium on the future of the International Latitude Service (I. L. S.) is to be held in Helsinki in July 1960. My report for the symposium consists of two parts. Part I, denoded (Mk I) was published [1] earlier in 1960 under the title “Latitude and Longitude, and the Secular Motion of the Pole”. Part II is the present paper, denoded (Mk II).

Download Full-text

Time and Latitude in South Africa

International Astronomical Union Colloquium ◽

10.1017/s0252921100026816 ◽

1972 ◽

Vol 1 ◽

pp. 27-38

Author(s):

J. Hers

Keyword(s):

South Africa ◽

Cape Town ◽

Astronomical Observations ◽

Royal Observatory ◽

The Republic ◽

Astronomical Determination ◽

Limited Accuracy ◽

Better Than

In South Africa the modern outlook towards time may be said to have started in 1948. Both the two major observatories, The Royal Observatory in Cape Town and the Union Observatory (now known as the Republic Observatory) in Johannesburg had, of course, been involved in the astronomical determination of time almost from their inception, and the Johannesburg Observatory has been responsible for the official time of South Africa since 1908. However the pendulum clocks then in use could not be relied on to provide an accuracy better than about 1/10 second, which was of the same order as that of the astronomical observations. It is doubtful if much use was made of even this limited accuracy outside the two observatories, and although there may – occasionally have been a demand for more accurate time, it was certainly not voiced.

Download Full-text