scholarly journals Temporary immersion bioreactor system for propagation by somatic embryogenesis of hybrid Larch (Larix × eurolepis Henry)

2021 ◽  
pp. e00684
Author(s):  
Kim-Cuong Le ◽  
Beata Dedicova ◽  
Sofie Johansson ◽  
Marie-Anne Lelu-Walter ◽  
Ulrika Egertsdotter
Keyword(s):  
Somatic Embryogenesis ◽  
Hybrid Larch ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion ◽  
Bioreactor System

Efficient production of virus-free apple plantlets using the temporary immersion bioreactor system

2020 ◽  
Vol 61 (4) ◽  
pp. 779-785
Author(s):  
Na-Young Kim ◽  
Ho-Dong Hwang ◽  
Jin-Ho Kim ◽  
Bo-Min Kwon ◽  
Daeil Kim ◽  
...  
Keyword(s):  
Efficient Production ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion ◽  
Bioreactor System

scholarly journals An Efficient Method of Pennisetum x Advena ‘Rubrum’ Seedlings Production Using Temporary Immersion Bioreactor and Agar Cultures

2021 ◽  
Author(s):  
Mariusz Pożoga ◽  
Dawid Olewnicki ◽  
Elżbieta Wójcik-Gront
Keyword(s):  
Liquid Medium ◽  
Efficient Method ◽  
Activated Charcoal ◽  
Agar Medium ◽  
Ms Medium ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion ◽  
Bright Green ◽  
Bioreactor System

Abstract The aim of this study was to propose an efficient method of Pennisetum x advena ‘Rubrum’ micropropagation. Agar cultures with MS medium supplemented with BAP in various concentrations (0.5 mg/L-2 mg/L) and a temporary immersion bioreactor system (TIS) with liquid medium MS with an addition of 1 mg/L BAP were used. For rooting ½ MS medium with different auxin combinations (IBA, NAA) and activated charcoal was utilized. The most efficient method turned out to be TIS which produced 36.9 new plants in four weeks. The seedlings were slender in shape, bright green in colour with no signs of hyperhydricity. The most suitable agar medium produced 19.5 new plants in an eight week period. Rooting should be carried on ½ MS supplemented with 0.5 mg/L IBA and 0.5 mg/L NAA with an 84% rooting rate. The addition of activated charcoal inhibited rooting.

scholarly journals Micropropagation of pineapple (Ananas comosus L. Var. Smooth cayenne) in temporary immersion bioreactor system (TIPS)

2021 ◽  
Vol 12 (2) ◽  
pp. 207-209
Author(s):  
L.A. Sani ◽  
I.S. Usman ◽  
A.U. Nasir ◽  
M.M. Abdulmalik
Keyword(s):  
Activated Charcoal ◽  
Ananas Comosus ◽  
Multiplication Rate ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion System ◽  
Temporary Immersion ◽  
The Family ◽  
Root Morphogenesis ◽  
Bioreactor System ◽  
Planting Materials

Pineapple is an important edible fruit in the family Bromeliaceae popularly grown in the tropical and subtropical countries. Commercial prodution of pineapple requires large volume of planting materials which could not easily be obtained using conventional method of propagation. A protocol for mass propagation of pineapple (Ananas comosus L. var.smooth cayenne) using temporary immersion bioreactor system has been developed.The protocol involves four immersion cycles in Murashinge and Skoog (MS) media fortified with 1mg/L or 2mg/L 6-Benzylaminopurine (BAP) with or without 0.25g/L activated charcoal (AC). The highest multiplication rate (120 -130 plants/bottle) was obtained when media was fortified with 1mg/l or 2mg/L BAP alone. The presence of activated charcoal (AC) promoted root morphogenesis, resulting in significant increase in roots formation in BAP suplemented media. A combination of BAP with AC significantly increased the number of competent plants(20 – 30 plants/bottle) after four weeks of culture in temporary immersion system. The system is recommended for rapid and efficient micropropagation of pineapple.

scholarly journals A Pattern-Based Approach for Detecting Pneumatic Failures on Temporary Immersion Bioreactors

Sensors ◽  
2019 ◽  
Vol 19 (2) ◽  
pp. 414 ◽  
Author(s):  
Octavio Loyola-González ◽  
Miguel Medina-Pérez ◽  
Dayton Hernández-Tamayo ◽  
Raúl Monroy ◽  
Jesús Carrasco-Ochoa ◽  
...  
Keyword(s):  
Production Cost ◽  
Biological Process ◽  
Feature Representation ◽  
Pneumatic System ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion ◽  
System A ◽  
Contrast Pattern ◽  
Bioreactor System ◽  
Feature Values

Temporary Immersion Bioreactors (TIBs) are used for increasing plant quality and plant multiplication rates. These TIBs are actioned by mean of a pneumatic system. A failure in the pneumatic system could produce severe damages into the TIB. Consequently, the whole biological process would be aborted, increasing the production cost. Therefore, an important task is to detect failures on a temporary immersion bioreactor system. In this paper, we propose to approach this task using a contrast pattern based classifier. We show that our proposal, for detecting pneumatic failures in a TIB, outperforms other approaches reported in the literature. In addition, we introduce a feature representation based on the differences among feature values. Additionally, we collected a new pineapple micropropagation database for detecting four new types of pneumatic failures on TIBs. Finally, we provide an analysis of our experimental results together with experts in both biotechnology and pneumatic devices.

Optimizing factors affecting development and propagation of Bletilla striata in a temporary immersion bioreactor system

2018 ◽  
Vol 232 ◽  
pp. 121-126 ◽  
Author(s):  
Baoqian Zhang ◽  
Lian Song ◽  
Lemma Dadi Bekele ◽  
Jingshan Shi ◽  
Qi Jia ◽  
...  
Keyword(s):  
Temporary Immersion Bioreactor ◽  
Bletilla Striata ◽  
Temporary Immersion ◽  
Factors Affecting ◽  
Bioreactor System

scholarly journals Bazı Turunçgil Anaçlarının Klasik ve Yeni Nesil Doku Kültürü Teknikleri ile Mikroçoğaltımı

2019 ◽  
Vol 7 (9) ◽  
pp. 1469
Author(s):  
Melike Cengiz ◽  
Yıldız Aka Kaçar
Keyword(s):  
Culture Medium ◽  
Woody Plant ◽  
Naphthaleneacetic Acid ◽  
Solid Culture ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion ◽  
Rooting Medium ◽  
Solid Culture Medium ◽  
Isopentenyl Adenine ◽  
Bioreactor System

In this study, micropropagation and rooting of ‘Tuzcu 31-31 sour orange’ and ‘C-35 citrange’ citrus rootstocks were conducted by comparing with Plantform temporary immersion bioreactor system and traditional solid culture. Murashige and Skoog Medium (MS) and Woody Plant Medium (WPM) supplemented with 6-Benzylaminopurine (BAP) (0, 1.0, 2.0 mg L-1), Kinetin (KIN) (0, 0.5, 1.0 mg L-1) and 2-Isopentenyl adenine (2IP) (0, 1.0, 2.0 mg L-1) were used in solid culture experiments. For solid culture rooting experiments, MS, ½ MS and WPM media supplemented with different concentrations of 1-Naphthaleneacetic acid (NAA) (0, 0.5, 1.0, 2.0 mg L-1) and Indole-3-butyric acid (IBA) (0, 0.5, 1.0, 2.0 mg L-1) were used. In both genotypes, the best micropropagation and rooting results were obtained from MS medium containing 2.0 mg L-1 BAP and ½ MS nutrient medium containing 0.5 mg L-1 NAA, respectively. Plantform bioreactor system was studied with the best medium content determined for micropropagation and rooting. As a result of the study, Plantform system gave better results in terms of plant quality in the micropropagation medium for both genotypes. Plantform system in rooting medium was found to be more advantageous than solid culture medium. As a result of the screening with SSR markers, it was determined that there was no somaclonal variation in the plants micropropagated and rooted in Plantform system.

scholarly journals THE USE OF CONTINUOUS, TEMPORARY IMMERSION BIOREACTOR SYSTEM AND SEMISOLID CULTURE MEDIUM FOR THE PRODUCTION Of Eucalyptus camaldulensis CLONES

2016 ◽  
Vol 26 (4) ◽  
pp. 1211 ◽  
Author(s):  
Evânia Galvão Mendonça ◽  
Vanessa Cristina Stein ◽  
Humberto Henrique De Carvalho ◽  
Breno Régis Santos ◽  
Luiz Alberto Beijo ◽  
...  
Keyword(s):  
Culture Medium ◽  
Eucalyptus Camaldulensis ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion ◽  
Bioreactor System

A micropropagação em sistemas de biorreatores é considerada como uma forma de reduzir os custos de produção por meio do escalonamento de automatização do processo. O objetivo desse trabalho foi desenvolverum protocolo eficiente de produção de mudas de Eucalyptus camaldulensis em diferentes tipos de sistema, incluindo biorreator de imersão continua e temporária. Para isso, meristemas apicais (1 mm) e meristemas apicais com tecido adjacente (2,5 mm) foram usados como explantes iniciais. Esses tecidos foram cultivados, por 60 dias, em meio de cultura suplementado com 1 mg L-1 de ácido indolacético (AIA) e 0.32 mg L-1 de benzilaminopurina (BAP). Após 60 dias, os meristemas com tecidos adjacentes foram transferidos para biorreatores de imersão contínua ou temporária e mantidos no escuro ou sob condições controladas de luminosidade. Para verificar o efeito da fonte de explante na multiplicação em biorreator foram testados explantes subcultivados de meristemas multiplicados em meio de cultura semissólido e meristemas multiplicados em biorreator de imersão contínua e mantidos no escuro. Despois de estabelecer esses parâmetros, os experimentos de multiplicação foram realizados em biorreatores de imersão contínua e temporária. Os explantes multiplicados foram enraizados em meio de cultura MS suplementado com 0, 2, 4, 8 e 20 mg L-1 de ácido indolbutírico (AIB) e mantidos no escuro ou sob condições controladas de luminosidade. Depois do enraizamento as plantas foram aclimatizadas em câmara de nebulização. Os meristemas com tecidos adjacentes favoreceram um maior número de gemas/explantes. O biorreator de imersão contínua e mantido no escuro promoveu maior número de brotações e maior taxa de multiplicação e o melhor enraizamento ocorreram no meio de cultura isento de auxina, mantido no escuro por 15 dias ou o meio de cultura suplementado com auxina, mantido na luz apresentando 100% de enraizamento. A aclimatização do Eucalyptus camaldulensis foi eficiente com taxa de sobrevivência de 76%. Portanto, foi possível desenvolver um método eficiente de micropropagação em biorreator para a produção de mudas Eucalyptus camaldulensis em larga escala.

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