Role of DNA hypomethylation in the development of the resistance to doxorubicin in human MCF-7 breast adenocarcinoma cells

2006 ◽  
Vol 231 (1) ◽  
pp. 87-93 ◽  
Author(s):  
Vasil F. Chekhun ◽  
Galina I. Kulik ◽  
Olga V. Yurchenko ◽  
Volodymyr P. Tryndyak ◽  
Igor N. Todor ◽  
...  
Keyword(s):  
Breast Adenocarcinoma ◽  
Dna Hypomethylation ◽  
Adenocarcinoma Cells ◽  
Resistance To Doxorubicin ◽  
Mcf 7

scholarly journals Estrogen-Responsive Genes Overlap with Triiodothyronine-Responsive Genes in a Breast Carcinoma Cell Line

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Nancy Bueno Figueiredo ◽  
Sílvia Helena Cestari ◽  
Sandro José Conde ◽  
Renata Azevedo Melo Luvizotto ◽  
Maria Teresa De Sibio ◽  
...  
Keyword(s):  
Breast Carcinoma ◽  
Dna Microarrays ◽  
Expression Patterns ◽  
Breast Adenocarcinoma ◽  
Breast Carcinoma Cell Line ◽  
Expression Of Genes ◽  
Adenocarcinoma Cells ◽  
Pericentriolar Material ◽  
Mcf 7

It has been well established that estrogen plays an important role in the progression and treatment of breast cancer. However, the role of triiodothyronine (T3) remains controversial. We have previously shown its capacity to stimulate the development of positive estrogen receptor breast carcinoma, induce the expression of genes (PR, TGF-alpha) normally stimulated by estradiol (E2), and suppress genes (TGF-beta) normally inhibited by E2. Since T3regulates growth hormones, metabolism, and differentiation, it is important to verify its action on other genes normally induced by E2. Therefore, we used DNA microarrays to compare gene expression patterns in MCF-7 breast adenocarcinoma cells treated with E2and T3. Several genes were modulated by both E2and T3in MCF-7 cells (Student’st-test,P<0.05). Specifically, we found eight genes that were differentially expressed after treatment with both E2and T3, includingamphiregulin, fibulin 1, claudin 6, pericentriolar material 1, premature ovarian failure 1B, factor for adipocyte differentiation-104, sterile alpha motif domain containing 9, and likely ortholog of rat vacuole membrane protein 1(fold change > 2.0, pFDR < 0.05). We confirmed our microarray results by real-time PCR. Our findings reveal that certain genes in MCF-7 cells can be regulated by both E2and T3.

The role of protein oxidative modification and the cellular redox status in realization of apoptosis of MCF-7 breast adenocarcinoma cells

2016 ◽  
Vol 43 (5) ◽  
pp. 385-389
Author(s):  
E. V. Shakhristova ◽  
E. A. Stepovaya ◽  
N. V. Ryazantseva ◽  
O. L. Nosareva ◽  
V. D. Yakushina ◽  
...  
Keyword(s):  
Redox Status ◽  
Oxidative Modification ◽  
Breast Adenocarcinoma ◽  
Cellular Redox ◽  
Protein Oxidative Modification ◽  
Adenocarcinoma Cells ◽  
Mcf 7

scholarly journals Change in the Expression of BAK، FAS، BAX، TNF-A، BCL-2 and Survivin Apoptosis Genes of the Human Breast Adenocarcinoma Cells (MCF-7) by Staphylococcal Enterotoxin B

2019 ◽  
Author(s):  
Sara Afzali ◽  
Abbas Doosti ◽  
Mansour Heidari ◽  
Nahid Babaei ◽  
Parvaneh Keshavarz
Keyword(s):  
Staphylococcal Enterotoxin ◽  
Breast Adenocarcinoma ◽  
Control Group ◽  
Staphylococcal Enterotoxin B ◽  
Type B ◽  
Human Breast ◽  
Human Breast Adenocarcinoma ◽  
Adenocarcinoma Cells ◽  
Enterotoxin B ◽  
Mcf 7

Introduction: Breast cancer is one of the most prevalent malignancies among women. Patients whose suffering from this condition, as a result of the use of conventional therapies often have a poor response to treatment and the relapse among them is frequent. In this study, the effects of staphylococcal enterotoxin type B on BAK، FAS، BAX، TNF-a، BCL-2 و Survivin genes expression in human breast adenocarcinoma cells (MCF-7) was examined. Staphylococcal enterotoxin B is a powerful member of the Staphylococcus aureus toxins family, which is known as an anticancer agent with potential for killing cancer cells. Methods: The experimental study was carried out at the Biotechnology Research Center of Islamic Azad University, Shahrekord Branch. By using Lipofectamine 2000 reagent, MCF-7  cells transfected with the pcDNA3.1(+)-seb (recombinant) and  pcDNA3.1(+) (non-recombinant)  plasmids and were selected by culturing in a selective medium of RPMI- 1640 containing 600 μg / mL antibiotic G418. Then, the expression of BAK, FAS, BAX, TNF-a, BCL-2, and Survivin genes in transfected cells were analyzed by real time PCR. Student's t-test, SPSS Inc., Chicago, IL; Version 16 and also Excel program for statistical analysis were used. Results: The results of this study indicated that staphylococcal enterotoxin type B (SEB) remarkably changes the expression of apoptotic related genes in MCF-7 cell line. It was observed a significant increase in the expression of BAK, FAS, BAX, and TNF-a genes, the expression of BCL-2 and Survivin genes significantly decreased compared to the control group (P=0/032). Conclusion: Staphylococcal enterotoxin type B has an inhibitory effect on the growth, proliferation and invasion of breast adenocarcinoma cells through altering the expression of the genes involved in the apoptosis process. Therefore, it seems that there is a good research field for the use of this toxin in the control and treatment of human breast adenocarcinoma.

scholarly journals In vitro veranderinge in mitochondriale membraan potensiaal, aggresoom formasie en kaspase aktiwiteit deur `n nuwe 17-β-estradiol analoog in bors adenokarsinoomselle

2012 ◽  
Vol 31 (1) ◽  
Author(s):  
Sandra Nkandeu ◽  
Thandi V. Mqoco ◽  
Michelle H. Visagie ◽  
Sumari Marais ◽  
Barend A. Stander ◽  
...  
Keyword(s):  
Exposure Time ◽  
Breast Adenocarcinoma ◽  
Caspase Activity ◽  
Mitochondrial Potential ◽  
Adenocarcinoma Cells ◽  
Aggresome Formation ◽  
Mcf 7

In vitro changes in mitochondrial potential, aggresome formation and caspase activity by a novel 17-beta-estradiol analog in mcf-7 breast adenocarcinoma cells. After a 24 hour exposure time, cells both apoptosis and autophagy were induced.

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