Radiotherapy quality insurance by individualized in vivo dosimetry: State of the art

This book presents a critical assessment of progress on the use of nuclear magnetic resonance spectroscopy to determine the structure of proteins, including brief reviews of the history of the field along with coverage of current clinical and in vivo applications. The book, in honor of Oleg Jardetsky, one of the pioneers of the field, is edited by two of the most highly respected investigators using NMR, and features contributions by most of the leading workers in the field. It will be valued as a landmark publication that presents the state-of-the-art perspectives regarding one of today's most important technologies.

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State-of-the-art methods and devices for generation, exposure, and collection of aerosols from e-vapor products

Toxicology Research and Application ◽

10.1177/2397847320979751 ◽

2020 ◽

Vol 4 ◽

pp. 239784732097975

Author(s):

Stéphanie Boué ◽

Didier Goedertier ◽

Julia Hoeng ◽

Anita Iskandar ◽

Arkadiusz K Kuczaj ◽

...

Keyword(s):

Ad Hoc ◽

State Of The Art ◽

Physicochemical Characterization ◽

Objective Evaluation ◽

Vapor Generation ◽

Characterization Methods ◽

Clinical Exposure ◽

Aerosol Generation

E-vapor products (EVP) have become popular alternatives for cigarette smokers who would otherwise continue to smoke. EVP research is challenging and complex, mostly because of the numerous and rapidly evolving technologies and designs as well as the multiplicity of e-liquid flavors and solvents available on the market. There is an urgent need to standardize all stages of EVP assessment, from the production of a reference product to e-vapor generation methods and from physicochemical characterization methods to nonclinical and clinical exposure studies. The objective of this review is to provide a detailed description of selected experimental setups and methods for EVP aerosol generation and collection and exposure systems for their in vitro and in vivo assessment. The focus is on the specificities of the product that constitute challenges and require development of ad hoc assessment frameworks, equipment, and methods. In so doing, this review aims to support further studies, objective evaluation, comparison, and verification of existing evidence, and, ultimately, formulation of standardized methods for testing EVPs.

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Bioengineered Islet Cell Transplantation

Current Transplantation Reports ◽

10.1007/s40472-021-00318-1 ◽

2021 ◽

Author(s):

Kevin Bellofatto ◽

Beat Moeckli ◽

Charles-Henri Wassmer ◽

Margaux Laurent ◽

Graziano Oldani ◽

...

Keyword(s):

State Of The Art ◽

Diabetes Research ◽

Islet Cell Transplantation ◽

Β Cell ◽

Cell Compartment ◽

Factors Affecting ◽

Insulin Activity ◽

On Chip

Abstract Purpose of Review β cell replacement via whole pancreas or islet transplantation has greatly evolved for the cure of type 1 diabetes. Both these strategies are however still affected by several limitations. Pancreas bioengineering holds the potential to overcome these hurdles aiming to repair and regenerate β cell compartment. In this review, we detail the state-of-the-art and recent progress in the bioengineering field applied to diabetes research. Recent Findings The primary target of pancreatic bioengineering is to manufacture a construct supporting insulin activity in vivo. Scaffold-base technique, 3D bioprinting, macro-devices, insulin-secreting organoids, and pancreas-on-chip represent the most promising technologies for pancreatic bioengineering. Summary There are several factors affecting the clinical application of these technologies, and studies reported so far are encouraging but need to be optimized. Nevertheless pancreas bioengineering is evolving very quickly and its combination with stem cell research developments can only accelerate this trend.

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In vivo interactome profiling by enzyme‐catalyzed proximity labeling

Cell & Bioscience ◽

10.1186/s13578-021-00542-3 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Yangfan Xu ◽

Xianqun Fan ◽

Yang Hu

Keyword(s):

State Of The Art ◽

Catalytic Efficiency ◽

Biological Processes ◽

Protein Protein Interaction ◽

Current State ◽

Protein Interactome ◽

Potential Applications ◽

Protein Protein Interaction Networks ◽

Temporal And Spatial

AbstractEnzyme-catalyzed proximity labeling (PL) combined with mass spectrometry (MS) has emerged as a revolutionary approach to reveal the protein-protein interaction networks, dissect complex biological processes, and characterize the subcellular proteome in a more physiological setting than before. The enzymatic tags are being upgraded to improve temporal and spatial resolution and obtain faster catalytic dynamics and higher catalytic efficiency. In vivo application of PL integrated with other state of the art techniques has recently been adapted in live animals and plants, allowing questions to be addressed that were previously inaccessible. It is timely to summarize the current state of PL-dependent interactome studies and their potential applications. We will focus on in vivo uses of newer versions of PL and highlight critical considerations for successful in vivo PL experiments that will provide novel insights into the protein interactome in the context of human diseases.

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Electro-Quasistatic Animal Body Communication for Untethered Rodent Biopotential Recording

Scientific Reports ◽

10.1038/s41598-021-81108-8 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Shreeya Sriram ◽

Shitij Avlani ◽

Matthew P. Ward ◽

Shreyas Sen

Keyword(s):

Lower Energy ◽

Sensor Node ◽

State Of The Art ◽

The Body ◽

Animal Body ◽

Current State ◽

Communication Modalities ◽

First Time ◽

Biopotential Recording

AbstractContinuous multi-channel monitoring of biopotential signals is vital in understanding the body as a whole, facilitating accurate models and predictions in neural research. The current state of the art in wireless technologies for untethered biopotential recordings rely on radiative electromagnetic (EM) fields. In such transmissions, only a small fraction of this energy is received since the EM fields are widely radiated resulting in lossy inefficient systems. Using the body as a communication medium (similar to a ’wire’) allows for the containment of the energy within the body, yielding order(s) of magnitude lower energy than radiative EM communication. In this work, we introduce Animal Body Communication (ABC), which utilizes the concept of using the body as a medium into the domain of untethered animal biopotential recording. This work, for the first time, develops the theory and models for animal body communication circuitry and channel loss. Using this theoretical model, a sub-inch$$^3$$ 3 [1″ × 1″ × 0.4″], custom-designed sensor node is built using off the shelf components which is capable of sensing and transmitting biopotential signals, through the body of the rat at significantly lower powers compared to traditional wireless transmissions. In-vivo experimental analysis proves that ABC successfully transmits acquired electrocardiogram (EKG) signals through the body with correlation $$>99\%$$ > 99 % when compared to traditional wireless communication modalities, with a 50$$\times$$ × reduction in power consumption.

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The use of prompt γ-rays for in-vivo dosimetry at therapeutic proton and ion beams

2011 IEEE Nuclear Science Symposium Conference Record ◽

10.1109/nssmic.2011.6152493 ◽

2011 ◽

Cited By ~ 5

Author(s):

F. Fiedler ◽

U. Dersch ◽

C. Golnik ◽

T. Kormoll ◽

A. Muller ◽

...

Keyword(s):

Ion Beams ◽

In Vivo Dosimetry

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Small footprint optoelectrodes using ring resonators for passive light localization

Microsystems & Nanoengineering ◽

10.1038/s41378-021-00263-0 ◽

2021 ◽

Vol 7 (1) ◽

Author(s):

Vittorino Lanzio ◽

Gregory Telian ◽

Alexander Koshelev ◽

Paolo Micheletti ◽

Gianni Presti ◽

...

Keyword(s):

State Of The Art ◽

Three Dimensional ◽

Network Activity ◽

Ring Resonators ◽

Spatiotemporal Resolution ◽

Optical Ring Resonators ◽

Light Localization ◽

Order Of Magnitude ◽

Small Footprint

AbstractThe combination of electrophysiology and optogenetics enables the exploration of how the brain operates down to a single neuron and its network activity. Neural probes are in vivo invasive devices that integrate sensors and stimulation sites to record and manipulate neuronal activity with high spatiotemporal resolution. State-of-the-art probes are limited by tradeoffs involving their lateral dimension, number of sensors, and ability to access independent stimulation sites. Here, we realize a highly scalable probe that features three-dimensional integration of small-footprint arrays of sensors and nanophotonic circuits to scale the density of sensors per cross-section by one order of magnitude with respect to state-of-the-art devices. For the first time, we overcome the spatial limit of the nanophotonic circuit by coupling only one waveguide to numerous optical ring resonators as passive nanophotonic switches. With this strategy, we achieve accurate on-demand light localization while avoiding spatially demanding bundles of waveguides and demonstrate the feasibility with a proof-of-concept device and its scalability towards high-resolution and low-damage neural optoelectrodes.

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