Preparation and characterization of molecular weight fractions of glycosaminoglycan from sea cucumber Thelenata ananas using free radical depolymerization

2010 ◽  
Vol 345 (5) ◽  
pp. 649-655 ◽  
Author(s):  
Mingyi Wu ◽  
Shimin Xu ◽  
Jinhua Zhao ◽  
Hui Kang ◽  
Hui Ding
Keyword(s):  
Molecular Weight ◽  
Free Radical ◽  
Sea Cucumber ◽  
Molecular Weight Fractions

scholarly journals α-Crystallin. The isolation and characterization of distinct macromolecular fractions

1971 ◽  
Vol 124 (2) ◽  
pp. 337-343 ◽  
Author(s):  
Abraham Spector ◽  
Lu-Ku Li ◽  
Robert C. Augusteyn ◽  
Arthur Schneider ◽  
Thomas Freund
Keyword(s):  
Molecular Weight ◽  
Amino Acid ◽  
Gel Filtration ◽  
Deae Cellulose ◽  
Chemical Difference ◽  
Molecular Weights ◽  
Isolation And Characterization ◽  
Different Populations ◽  
Molecular Weight Fractions

α-Crystallin was isolated from calf lens periphery by chromatography on DEAE-cellulose and gel filtration. Three distinct populations of macromolecules have been isolated with molecular weights in the ranges approx. 6×105−9×105, 0.9×106−4×106and greater than 10×106. The concentration of macromolecules at the molecular-weight limits of a population are very low. The members of the different populations do not appear to be in equilibrium with each other. Further, in those molecular-weight fractions investigated, no equilibrium between members of the same population was observed. The population of lowest molecular weight comprises 65–75% of the total material. The amino acid and subunit composition of the different-sized fractions appear very similar, if not identical. The only chemical difference observed between the fractions is the presence of significant amounts of sugar in the higher-molecular-weight fractions. Subunit molecular weights of approx. 19.5×103and 22.5×103were observed for all α-crystallin fractions.

Molecular Weight Characterization of Poly(N-isopropylacrylamide) Prepared by Living Free-Radical Polymerization

2000 ◽  
Vol 33 (18) ◽  
pp. 6738-6745 ◽  
Author(s):  
François Ganachaud ◽  
Michael J. Monteiro ◽  
Robert G. Gilbert ◽  
Marie-Anne Dourges ◽  
San H. Thang ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Free Radical ◽  
Radical Polymerization ◽  
Free Radical Polymerization ◽  
Living Free Radical Polymerization

scholarly journals Extraction and Characterization of Collagen from Sand Sea Cucumber (Holothuria scabra)

2021 ◽  
Vol 26 (3) ◽  
pp. 319-327
Author(s):  
Gita Syahputra ◽  
Hariyatun Hariyatun ◽  
Muhammad Firdaus ◽  
Pugoh Santoso
Keyword(s):  
Molecular Weight ◽  
Sea Cucumber ◽  
Type I ◽  
Indonesian Seas ◽  
Holothuria Scabra ◽  
Aquatic Product ◽  
Electron Microscopy Analysis ◽  
Main Component ◽  
Almost All

Sand sea cucumber (Holothuria scabra) is an aquatic product that belongs to Echinodermata, a habitant in almost all Indonesian seas. The main component of the sea cucumber is protein, one of which is collagen. This study aimed to extract and characterize collagen from the species using the acid-base extraction method. The characterization of sea cucumber collagen includes molecular weight, amino acid components, Fourier transform infrared spectrophotometry, and scanning electron microscopy analysis. This study has successfully extracted collagen from the sample using an extraction system: NaOH 0.1 M; CH3COOH 0.1 M; and distilled water under 45°C treatments, gave 6% yield. The collagen has a molecular weight 110-130 kDa. Based on the infrared spectra, the specific functional groups of the collagen are amide A (3379.29 cm-1), amide B (2924.09 cm-1), amide I (1681.93 cm-1), amide II (1560.41 cm-1), and amide III (1249.87 cm-1). The collagen falls into type I. We suggest an alternative resource of collagen from sand sea cucumber, other than poultry and mammals.   Keywords: characterization, collagen, extraction, fishery, sand sea cucumber

scholarly journals Characterization of uterine heterogeneous nuclear ribonucleic acid and the effect of oestradiol-17β on its synthesis

1978 ◽  
Vol 172 (3) ◽  
pp. 587-593 ◽  
Author(s):  
S Aziz ◽  
J T Knowler
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Early Response ◽  
Oestrogen Treatment ◽  
Size Profile ◽  
Immature Rats ◽  
Nuclear Rna ◽  
Kinetics Of ◽  
Molecular Weight Fractions

An early response to the administration of oestradiol-17 beta to immature rats is a dramatic stimulation in the synthesis of uterine hnRNA (heterogenous nuclear RNA). High-molecular-weight fractions of the hnRNA were purified and subfractionated on poly(U)-Sepharose into fractions that differed in their poly(A) content and their size profile on polyacrylamide gels. Oestrogen treatment of the rats stimulated the synthesis of all three fractions of high-molecular-weight hnRNA, but the kinetics of synthesis, degree of stimulation and size distribution of the newly synthesize RNA differed in each fraction.

scholarly journals Using free radical polymerization and Mannich reaction, synthesis and characterization of cationic polyacrylamides having similar molecular weight but different charge densities

2018 ◽  
Vol 9 (2) ◽  
pp. 79-83 ◽  
Author(s):  
Raid Saleh Shatat ◽  
Shaik Kalimulla Niazi
Keyword(s):  
Molecular Weight ◽  
Free Radical ◽  
Mannich Reaction ◽  
Solution Polymerization ◽  
Similar Molecular Weight ◽  
Charge Densities ◽  
Polyacrylamide Solution ◽  
Acrylamide Monomer ◽  
Radical Solution

Using free radical solution polymerization technique and Mannich reaction, five different polyacrylamides with similar molecular weight but variable charge densities were synthesized. High molecular weight polyacrylamides were synthesized using potassium persulfate and N,N,N',N'-tetramethylethylenediamine system as initiators. This was achieved by increasing the concentration of acrylamide monomer. They were then characterized by infrared spectroscopy, viscosity measurements and glass transition temperature measurement. These compounds with various cationic charge densities from 48.2, 161.7, 355, 425 and 485 C/g were prepared through Mannich reaction. The results indicate, by increasing the acidity of the polyacrylamide solution using sulfuric acid, the pH of the polyacrylamide solution decreases correspondingly. As a result, the positive charges increased resulting in the enrichment of charge densities.

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