scholarly journals Extraction and Characterization of Collagen from Sand Sea Cucumber (Holothuria scabra)

2021 ◽  
Vol 26 (3) ◽  
pp. 319-327
Author(s):  
Gita Syahputra ◽  
Hariyatun Hariyatun ◽  
Muhammad Firdaus ◽  
Pugoh Santoso
Keyword(s):  
Molecular Weight ◽  
Sea Cucumber ◽  
Type I ◽  
Indonesian Seas ◽  
Holothuria Scabra ◽  
Aquatic Product ◽  
Electron Microscopy Analysis ◽  
Main Component ◽  
Almost All

Sand sea cucumber (Holothuria scabra) is an aquatic product that belongs to Echinodermata, a habitant in almost all Indonesian seas. The main component of the sea cucumber is protein, one of which is collagen. This study aimed to extract and characterize collagen from the species using the acid-base extraction method. The characterization of sea cucumber collagen includes molecular weight, amino acid components, Fourier transform infrared spectrophotometry, and scanning electron microscopy analysis. This study has successfully extracted collagen from the sample using an extraction system: NaOH 0.1 M; CH3COOH 0.1 M; and distilled water under 45°C treatments, gave 6% yield. The collagen has a molecular weight 110-130 kDa. Based on the infrared spectra, the specific functional groups of the collagen are amide A (3379.29 cm-1), amide B (2924.09 cm-1), amide I (1681.93 cm-1), amide II (1560.41 cm-1), and amide III (1249.87 cm-1). The collagen falls into type I. We suggest an alternative resource of collagen from sand sea cucumber, other than poultry and mammals.   Keywords: characterization, collagen, extraction, fishery, sand sea cucumber

scholarly journals Purification and Characterization of an Extracellular Poly(l-Lactic Acid) Depolymerase from a Soil Isolate,Amycolatopsis sp. Strain K104-1

2001 ◽  
Vol 67 (1) ◽  
pp. 345-353 ◽  
Author(s):  
Kohei Nakamura ◽  
Toshio Tomita ◽  
Naoki Abe ◽  
Yoshiyuki Kamio
Keyword(s):  
Molecular Weight ◽  
Lactic Acid ◽  
Collagen Type I ◽  
Type I ◽  
Chloromethyl Ketone ◽  
Diisopropyl Fluorophosphate ◽  
Soil Isolate ◽  
Solid Film ◽  
Subtilisin Inhibitor

ABSTRACT Poly(l-lactic acid) (PLA)-degradingAmycolatopsis sp. strains K104-1 and K104-2 were isolated by screening 300 soil samples for the ability to form clear zones on the PLA-emulsified mineral agar plates. Both of the strains assimilated >90% of emulsified 0.1% (wt/vol) PLA within 8 days under aerobic conditions. A novel PLA depolymerase with a molecular weight of 24,000 was purified to homogeneity from the culture supernatant of strain K104-1. The purified enzyme degraded high-molecular-weight PLA in emulsion and in solid film, ultimately forming lactic acid. The optimum pH for the enzyme activity was 9.5, and the optimum temperature was 55 to 60°C. The PLA depolymerase also degraded casein and fibrin but did not hydrolyze collagen type I, triolein, tributyrin, poly(β-hydroxybutyrate), or poly(ε-caprolactone). The PLA-degrading and caseinolytic activities of the enzyme were inhibited by diisopropyl fluorophosphate and phenylmethylsulfonyl fluoride but were not significantly affected by soybean trypsin inhibitor,N-tosyl-l-lysyl chloromethyl ketone,N-tosyl-l-phenylalanyl chloromethyl ketone, and Streptomyces subtilisin inhibitor. Thus, Amycolatopsis sp. strain K104-1 excretes the unique PLA-degrading and fibrinolytic serine enzyme, utilizing extracellular polylactide as a sole carbon source.

Characterization of TRH/GnRH-like peptides in the sea cucumber, Holothuria scabra, and their effects on oocyte maturation

Aquaculture ◽  
2020 ◽  
Vol 518 ◽  
pp. 734814
Author(s):  
Arada Chaiyamoon ◽  
Ruchanok Tinikul ◽  
Nittiya Nontunha ◽  
Supakant Chaichotranunt ◽  
Tanes Poomtong ◽  
...  
Keyword(s):  
Oocyte Maturation ◽  
Sea Cucumber ◽  
Holothuria Scabra

Estimation of Discretionary Accruals and the Detection of Earnings Management

2015 ◽  
Author(s):  
Paul Zarowin
Keyword(s):  
Earnings Management ◽  
Discretionary Accruals ◽  
Type I ◽  
Type Ii ◽  
Type I Errors ◽  
New Model ◽  
The Past ◽  
Almost All ◽  
Type Ii Errors

This article reviews recent research on the estimation of discretionary accruals and the detection of earnings management. There has been an explosive growth in research on accrual earnings management over the past twenty years, and almost all has used the Jones (1991) model or one of its close derivatives. Nevertheless, a growing literature has addressed the model’s problems and attempted to improve its estimation of discretionary accruals. The model’s incomplete characterization of how nondiscretionary accruals are determined by the firm’s operations can cause either Type I or Type II errors. This article categorizes recent articles into four groups based on their focus and solution, and while there is no panacea for the problems and no consensus on a new model or method, research offers hope that accrual earnings management is more likely to be detected when it exists and is less likely to be erroneously detected when it is absent (i.e., lower Type II and Type I errors, respectively).

scholarly journals Isolation and characterization of polymorphic microsatellite loci in the sea cucumber Holothuria scabra

2015 ◽  
Vol 14 (2) ◽  
pp. 6529-6532 ◽  
Author(s):  
Z.B. Li ◽  
G. Dai ◽  
J.B. Shangguan ◽  
Y.F. Ning ◽  
Y.Y. Li ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Sea Cucumber ◽  
Holothuria Scabra ◽  
Isolation And Characterization ◽  
Polymorphic Microsatellite

scholarly journals Characterization of human placental neuraminidases. Stability, substrate specificity and molecular weight

1982 ◽  
Vol 205 (2) ◽  
pp. 345-351 ◽  
Author(s):  
D McNamara ◽  
G Beauregard ◽  
H V Nguyen ◽  
D L Yan ◽  
M Bélisle ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Substrate Specificity ◽  
Half Life ◽  
Fluorogenic Substrate ◽  
Ph Optimum ◽  
Preliminary Results ◽  
Neuraminidase Activity ◽  
Almost All ◽  
Gd1a Ganglioside

1. At least two components of neuraminidase can be distinguished on the basis of thermolability and sedimentability by using the artificial fluorogenic substrate 4-methylumbelliferyl N-acetyl-alpha-D-neuraminate. 2. In crude homogenates, thermodenaturation at 25 degrees C showed a biphasic curve corresponding to component A (half-life, 21 min) and B (half-life, 85 min). The two components were partially resolved by centrifugation. A being soluble and B sedimentable. Both had similar pH-activity curves (pH optimum, 4.4), Km values (A, 0.10 mM; B, 0.06 mM) and molecular weight as determined by radiation inactivation (A, 67000; B, 63000). 3. The soluble A form was still aggregated or bound to membranous debris since almost all neuraminidase activity was eluted near or at the void volume of a Sephacryl S-300 column. 4. Both soluble and sedimentable fractions of placenta hydrolysed the GD1A ganglioside and N-acetyl-neuraminyl-D-lactose linearly for 12 h but no fetuin hydrolysis was detected. 5. The neuraminidase activity with the artificial fluorogenic substrate was inhibited by N-acetylneuraminyl-D-lactose but not by the GD1A ganglioside. These preliminary results suggest that there exist two closely related enzymes hydrolysing both the artificial substrate and N-acetylneuraminyl-D-lactose and a third one hydrolysing the GD1A ganglioside exclusively.

scholarly journals Comparative Study of The Yield and Physicochemical Properties of Collagen from Sea Cucumber (Holothuria scabra), Obtained through Dialysis and the Ultrafiltration Membrane

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2564
Author(s):  
Suryani Saallah ◽  
Jumardi Roslan ◽  
Flavian Sheryl Julius ◽  
Sharinee Saallah ◽  
Umi Hartina Mohamad Razali ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Sea Cucumber ◽  
Large Scale ◽  
Type I Collagen ◽  
Ultrafiltration Membrane ◽  
The Body ◽  
Type I ◽  
Holothuria Scabra ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

Collagen was extracted from the body wall of sea cucumber (Holothuria scabra) using the pepsin-solubilized collagen method followed by isolation using dialysis and the ultrafiltration membrane. The yield and physicochemical properties of the collagen obtained from both isolation methods, denoted as D-PSC and UF-PSC, were compared. The ultrafiltration method affords a higher yield of collagen (11.39%) than that of the dialysis (5.15%). The isolated collagens have almost the same amino acid composition, while their functional groups, referred to as amide A, B, I, II, and III bands, were in accordance with commercial collagen, as verified by Fourier Transform Infrared (FT-IR) spectroscopy. The UV-Vis absorption peaks at 240 nm and 220 nm, respectively, indicated that the collagens produced are type-I collagen. The D-PSC showed interconnecting sheet-like fibrils, while the UF-PSC exhibited a flaky structure with flat-sheets arranged very close to each other. The higher yield and comparable physicochemical properties of the collagen obtained by ultrafiltration as compared with dialysis indicate that the membrane process has high potential to be used in large-scale collagen production for food and pharmaceutical applications.

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