Integration of small RNAs and mRNAs by high-throughput sequencing reveals a complex regulatory network in Chinese sea cucumber, Russian sea cucumber and their hybrids

2019 ◽  
Vol 29 ◽  
pp. 1-13 ◽  
Author(s):  
Zhicheng Wang ◽  
Jun Cui ◽  
Jian Song ◽  
Meng Gou ◽  
Haoze Wang ◽  
...  
Keyword(s):  
High Throughput ◽  
Regulatory Network ◽  
Small Rnas ◽  
Sea Cucumber ◽  
High Throughput Sequencing ◽  
Complex Regulatory Network

scholarly journals The high-throughput sequencing of small RNAs profiling in wide hybridisation and allopolyploidisation between Brassica rapa and Brassica nigra

Genomics Data ◽  
2015 ◽  
Vol 3 ◽  
pp. 1-3 ◽  
Author(s):  
Muhammad Awais Ghani ◽  
Junxing Li ◽  
Linli Rao ◽  
Muhammad Ammar Raza ◽  
Liwen Cao ◽  
...  
Keyword(s):  
Brassica Rapa ◽  
High Throughput ◽  
Small Rnas ◽  
High Throughput Sequencing ◽  
Brassica Nigra ◽  
Wide Hybridisation

scholarly journals Integration of Transcriptomes, Small RNAs, and Degradome Sequencing to Identify Putative miRNAs and their Targets Related to Eu-Rubber Biosynthesis in Eucommia ulmoides

Genes ◽  
2019 ◽  
Vol 10 (8) ◽  
pp. 623
Author(s):  
Jing Ye ◽  
Wenjing Han ◽  
Ruisheng Fan ◽  
Minhao Liu ◽  
Long Li ◽  
...  
Keyword(s):  
Regulatory Network ◽  
Small Rnas ◽  
High Throughput Sequencing ◽  
Expression Profiles ◽  
Vital Role ◽  
Eucommia Ulmoides ◽  
Messenger Rnas ◽  
Rubber Biosynthesis ◽  
Degradome Sequencing ◽  
The Eu

Eucommia ulmoides has attracted much attention as a valuable natural rubber (Eu-rubber) production tree. As a strategic material, Eu-rubber plays a vital role in general and defence industries. However, the study of Eu-rubber biosynthesis at a molecular level is scarce, and the regulatory network between microRNAs (miRNAs) and messenger RNAs (mRNAs) in Eu-rubber biosynthesis has not been assessed. In this study, we comprehensively analyzed the transcriptomes, small RNAs (sRNAs) and degradome to reveal the regulatory network of Eu-rubber biosynthesis in E. ulmoides. A total of 82,065 unigenes and 221 miRNAs were identified using high-throughput sequencing; 20,815 targets were predicted using psRNATarget software. Of these targets, 779 miRNA-target pairs were identified via degradome sequencing. Thirty-one miRNAs were differentially expressed; 22 targets of 34 miRNAs were annotated in the terpenoid backbone biosynthesis pathway (ko00900) based on the Kyoto Encyclopedia of Genes and Genomes (KEGG). These miRNAs were putatively related to Eu-rubber biosynthesis. A regulatory network was constructed according to the expression profiles of miRNAs and their targets. These results provide a comprehensive analysis of transcriptomics, sRNAs and degradome to reveal the Eu-rubber accumulation, and provide new insights into genetic engineering techniques which may improve the content of Eu-rubber in E. ulmoides.

scholarly journals Characterization of small RNAs from Ulva prolifera by high-throughput sequencing and bioinformatics analysis

2011 ◽  
Vol 56 (27) ◽  
pp. 2916-2921 ◽  
Author(s):  
AiYou Huang ◽  
GuangCe Wang ◽  
LinWen He ◽  
JianFeng Niu ◽  
BaoYu Zhang
Keyword(s):  
High Throughput ◽  
Small Rnas ◽  
High Throughput Sequencing ◽  
Bioinformatics Analysis ◽  
Ulva Prolifera

scholarly journals Construction and Analysis of an Integrated Regulatory Network Derived from High-Throughput Sequencing Data

2011 ◽  
Vol 7 (11) ◽  
pp. e1002190 ◽  
Author(s):  
Chao Cheng ◽  
Koon-Kiu Yan ◽  
Woochang Hwang ◽  
Jiang Qian ◽  
Nitin Bhardwaj ◽  
...  
Keyword(s):  
High Throughput ◽  
Regulatory Network ◽  
High Throughput Sequencing ◽  
Sequencing Data ◽  
High Throughput Sequencing Data
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