The effect of copper toxicity in apoptotic response and cell proliferation, in gills of Oreochromis niloticus

Background: Lectins have been studied in recent years due to their immunomodulatory activities. Objective: We purified a lectin named OniL from tilapia fish (Oreochromis niloticus) and here we analyzed the cell proliferation and cytokine production in Balb/c mice splenocytes. Methods: Cells were stimulated in vitro in 24, 48, 72 hours and 6 days with different concentrations of OniL and Con A. Evaluation of cell proliferation was performed through [3H]-thymidine incorporation, cytokines were investigated using ELISA assay and cell viability assay was performed by investigation of damage through signals of apoptosis and necrosis. Results: OniL did not promote significant cell death, induced high mitogenic activity in relation to control and Con A and stimulated the cells to release high IL-2 and IL-6 cytokines. Conclusion: These findings suggest that, like Con A, OniL lectin can be used as a mitogenic agent in immunostimulatory assays.

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The effect of copper toxicity on the contents of nitrogen compounds in Silene vulgaris (Moench) Garcke

Plant and Soil ◽

10.1007/bf00011904 ◽

1991 ◽

Vol 133 (1) ◽

pp. 101-109 ◽

Cited By ~ 20

Author(s):

M. B. Weber ◽

H. Schat ◽

W. M. Ten Bookum-Van Der Maarel

Keyword(s):

Copper Toxicity ◽

Nitrogen Compounds ◽

Silene Vulgaris ◽

Effect Of Copper

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Copper promotes sheep pancreatic duct organoid growth by activation of an antioxidant protein 1-dependent MEK-ERK pathway

AJP Cell Physiology ◽

10.1152/ajpcell.00509.2019 ◽

2020 ◽

Vol 318 (4) ◽

pp. C806-C816

Author(s):

Miao Liu ◽

Wen Yu ◽

Jing Jin ◽

Mingjun Ma ◽

Tiezhu An ◽

...

Keyword(s):

Cell Proliferation ◽

Pancreatic Duct ◽

Physical Growth ◽

Farm Animals ◽

Copper Chaperone ◽

Erk Pathway ◽

Growth Mechanisms ◽

Livestock Feed ◽

Antioxidant Protein ◽

Effect Of Copper

Proper amounts of copper supplemented in livestock feed improve the physical growth and traits of farm animals. The pancreas is an important organ with both exocrine and endocrine portions. To investigate the role and mechanism of copper in the sheep pancreas, we first established sheep pancreatic duct organoids (sPDOs). We found that an appropriate amount of copper benefited the formation and growth of sPDOs, whereas excess or deficient copper damaged sPDOs. We found that the proliferation-stimulating effect of copper was related to the copper chaperone antioxidant protein 1 (ATOX1)-dependent activation of MEK-ERK1/2 signaling. Atox1 knockdown suppressed the cell proliferation of sPDOs, even in the presence of the MEK activator. These results indicate that moderate concentrations of copper promote sPDO growth through ATOX1-regulated cell proliferation by activation of MEK-ERK. Moreover, our study indicates that organoids may be a useful model to study organ growth mechanisms in livestock.

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THE EFFECT OF COPPER ON DISTILLED WATER QUALITY FOR USE IN MILK AND WATER LABORATORIES

Journal of Milk and Food Technology ◽

10.4315/0022-2747-30.10.305 ◽

1967 ◽

Vol 30 (10) ◽

pp. 305-309 ◽

Cited By ~ 1

Author(s):

Gene W. Ronald ◽

R. L. Morris

Keyword(s):

Water Quality ◽

Milk Sample ◽

Bacterial Flora ◽

Copper Toxicity ◽

Test Organism ◽

Raw Milk ◽

Distilled Water ◽

Aerobacter Aerogenes ◽

Normal Bacterial Flora ◽

Effect Of Copper

Summary Levels of copper toxicity have been established in distilled water using the distilled water suitability test. It is shown that levels of copper, toxic by the distilled water suitability test, are not toxic to the test organism, Aerobacter aerogenes, in sterile milk or to the normal bacterial flora of a raw milk sample. It is the contention of this paper that the distilled water suitability test is an unrealistically severe yardstick of distilled water quality for use in routine milk and water laboratories.

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The Effect of Copper and Lead on Oreochromis niloticus and Cyprinus carpio in Blood Ion Level

Ekoloji ◽

10.5053/ekoloji.2013.877 ◽

2013 ◽

Vol 22 (87) ◽

pp. 51-57 ◽

Cited By ~ 1

Author(s):

Hikmet Yeter Cogun ◽

Ferit Kargin

Keyword(s):

Cyprinus Carpio ◽

Oreochromis Niloticus ◽

Effect Of Copper

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Effect of Copper Toxicity on Root Morphology, Ultrastructure, and Copper Accumulation in Moso Bamboo (Phyllostachys pubescens)

Zeitschrift für Naturforschung C ◽

10.5560/znc.2014-0022 ◽

2014 ◽

Vol 69 (9-10) ◽

pp. 399-406 ◽

Cited By ~ 8

Author(s):

Junren Chen ◽

Danli Peng ◽

Mohammad Shafi ◽

Song Li ◽

Jiasen Wu ◽

...

Keyword(s):

Electron Microscopy ◽

Root Morphology ◽

Copper Toxicity ◽

Dry Weight ◽

Moso Bamboo ◽

Copper Accumulation ◽

Root Surface Area ◽

Phyllostachys Pubescens ◽

Root Tips ◽

Effect Of Copper

Abstract A hydroponic culture experiment was conducted to study the effect of copper toxicity on root morphology, ultrastructure, and copper accumulation in Moso bamboo (Phyllostachys pubescens). Root ultrastructure of Moso bamboo was studied by transmission electron microscopy and scanning electron microscopy. Application of 200 μM Cu resulted in an accumulation of 810 mg kg-1 dry weight and 91 mg kg-1 dry weight Cu in roots and shoots, respectively. The majority of the plants did not survive the application of 400 μM Cu. Biomass production declined consistently with application of each additional increment of Cu. Root growth was more severely inhibited than shoot growth. Cu adversely affected the root morphology of the plants, however, root surface area and number of root tips increased slightly at low levels of Cu. Root cell ultrastructure and organelles changed significantly under Cu stress, in particular, cell walls, mitochondria, and xylem parenchyma were affected.

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