Alterations in hematological and biochemical parameters, oxidative stress, and immune response in Takifugu rubripes under acute ammonia exposure

This study is conducted to evaluate the therapeutic and antioxidant effect of lemon juice on some hematological and biochemical parameters. Thirty female mice used in this study were exposed to oxidative stress through giving them hydrogen peroxide in drinking water for 30 days. Animals randomly distributed over 3 groups, each group contained 10 animals and treated as follows: T1 control group (drinking distilled water only), T2 (0.75% hydrogen peroxide in drinking water) and T3 (0.75% hydrogen peroxide in drinking water with daily drenching with 1 mL lemon juice). At the end of the experiment, blood samples were collected from animals for evaluating the following hematological and biochemical parameters: Haemoglobin concentration (Hb), red blood cells count (RBC), white blood cells count (WBC), packed cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), platelet count (PLT), level of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and total protein. The results showed that T3 exhibited an enhancement in RBC count, Hb concentration, WBC, lymphocyte and total protein and reduction in the level of AST and ALT compared to T2. These findings clearly revealed the advance protective and antioxidant features of lemon juice on hematological and biochemical parameters of the oxidatively stressed female mice.

Download Full-text

Trigonella foenum-graecumprotection against deltamethrin-induced toxic effects on haematological, biochemical, and oxidative stress parameters in rats

Canadian Journal of Physiology and Pharmacology ◽

10.1139/cjpp-2014-0144 ◽

2014 ◽

Vol 92 (8) ◽

pp. 679-685 ◽

Cited By ~ 38

Author(s):

Mohamed M. Abdel-Daim ◽

Mabrouk A. Abd Eldaim ◽

Mohamed M. Mahmoud

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Body Mass ◽

Biochemical Parameters ◽

Toxic Effects ◽

Protective Agent ◽

Dependent Manner ◽

Hematological And Biochemical Parameters ◽

Trigonella Foenum Graecum L ◽

And Oxidative Stress

Trigonella foenum-graecum L. is enriched with many active ingredients. TFG oil was evaluated for its protective effect against deltamethrin toxicity in rats. Rats of the control group were administered saline. The 2nd group was administered deltamethrin (DLM) orally at a concentration of 15 mg/kg body mass. The 3rd and 4th groups were administered DLM at a concentration of 15 mg/kg body mass and were fed diets containing 2.5% and 5% TFG oil, respectively. DLM intoxication reduced red blood cell and platelet counts, hemoglobin concentration, and hematocrit value while it induced leucocytosis. Furthermore, it increased serum levels of lactate dehydrogenase, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, γ-glutamyltransferase, triglycerides, cholesterol, uric acid, urea, and creatinine; increased hepatic, renal, and brain lipid peroxidation; decreased serum acetylcholine esterase level; and decreased hepatic, renal, and brain antioxidant markers’ activities. However, TFG oil kept the studied hematological and biochemical parameters within normal ranges. In addition, it prevented lipid peroxidation and oxidative stress induced by DLM intoxication in a dose-dependent manner. Therefore, these results indicated that TFG oil inhibited the toxic effects of DLM on hematological and biochemical parameters as well as oxidative status by its free radical scavenging and potent antioxidant activities, and it appeared to be a promising protective agent against DLM-induced toxicity.

Download Full-text

Fucoidan Protects against Acute Sulfoxaflor-Induced Hematological/Biochemical Alterations and Oxidative Stress in Male Mice

Pharmaceuticals ◽

10.3390/ph15010016 ◽

2021 ◽

Vol 15 (1) ◽

pp. 16

Author(s):

Petek Piner Benli ◽

Merve Kaya ◽

Yusuf Kenan Dağlıoğlu

Keyword(s):

Oxidative Stress ◽

Biochemical Parameters ◽

Biological Activities ◽

Hematological Parameters ◽

Sulfated Polysaccharide ◽

Protective Role ◽

Protective Effects ◽

Biochemical Alterations ◽

Hematological And Biochemical Parameters ◽

And Oxidative Stress

Fucoidan is a sulfated polysaccharide which can be found among a number of macroalgea species. It has a broad spectrum of biological activities including anti-oxidant, anti-tumor, immunoregulation, anti-viral and anti-coagulant. The current study was performed to investigate possible protective effects of fucoidan for sulfoxaflor-induced hematological/biochemical alterations and oxidative stress in the blood of male Swiss albino mice. For this purpose, sulfoxaflor was administered at a dose of 15 mg/kg/day (1/50 oral LD50), and fucoidan was administered at a dose of 50 mg/kg/day by oral gavage alone and combined for 24 h and 7 days. Hematological parameters (RBC, HGB, HCT, MCV, MCH, MCHC, Plt, WBC, Neu, Lym and Mon), serum biochemical parameters (AST, ALT, GGT, LDH, BUN, Cre and TBil), and serum oxidative stress/antioxidant markers (8-OHdG, MDA, POC and GSH) were analyzed. The results indicated that sulfoxaflor altered hematological and biochemical parameters and caused oxidative stress in mice; fucoidan ameliorated some hematological and biochemical parameters and exhibited a protective role as an antioxidant against sulfoxaflor-induced oxidative stress.

Download Full-text

Hematological and Biochemical Parameters in Hereditary Spherocytosis Under Oxidative Stress

Molecular Biology of Hematopoiesis 6 ◽

10.1007/978-1-4615-4797-6_41 ◽

1999 ◽

pp. 337-345

Author(s):

Elisa Granjo ◽

Alice Santos-Silva ◽

Irene Rebelo ◽

Ana Nóvoa ◽

Elisio Costa ◽

...

Keyword(s):

Oxidative Stress ◽

Biochemical Parameters ◽

Hereditary Spherocytosis ◽

Hematological And Biochemical Parameters

Download Full-text

The effects of ammonia exposure on immune response, oxidative stress, and apoptosis in Procambarus clarkii

Aquaculture International ◽

10.1007/s10499-021-00828-w ◽

2022 ◽

Author(s):

Yaqi Luo ◽

Chenchen Shen ◽

Dan Tang ◽

Yuze Bai ◽

Lv Wu ◽

...

Keyword(s):

Oxidative Stress ◽

Immune Response ◽

Procambarus Clarkii ◽

Ammonia Exposure

Download Full-text

Evaluation of oxidative stress, hematological and biochemical parameters during Toxoplasma gondii infection in gerbils

Ankara Üniversitesi Veteriner Fakültesi Dergisi ◽

10.1501/vetfak_0000002675 ◽

2015 ◽

Vol 62 (3) ◽

pp. 165-170 ◽

Cited By ~ 1

Author(s):

ATMACA Nurgül; ÇINAR

Keyword(s):

Oxidative Stress ◽

Toxoplasma Gondii ◽

Biochemical Parameters ◽

Hematological And Biochemical Parameters ◽

Toxoplasma Gondii Infection

Download Full-text

Bioaccumulation, Oxidative Stress, Immune Responses in Channa Argus Exposure to Waterborne Hexavalent Chromium

10.21203/rs.3.rs-633529/v1 ◽

2021 ◽

Author(s):

Jia-hua du ◽

Zhe Yu ◽

Jun-liang Zhao ◽

Zhi-hui Bai ◽

Ze-hao Guo ◽

...

Keyword(s):

Oxidative Stress ◽

Immune Response ◽

Immune Responses ◽

Hexavalent Chromium ◽

Biochemical Parameters ◽

Immunoglobulin M ◽

Environmental Toxicants ◽

The World ◽

Channa Argus ◽

And Oxidative Stress

Abstract Hexavalent chromium (Cr (VI)) is a very common and harmful heavy metal pollutant in the world. However, the responses of Cr (VI) to aquatic environmental toxicants have not been well described. In this study, we evaluated the waterborne Cr (VI) [ 0 mg/L (C1), 0.5 mg/L (C2), 1 mg/L (C3), 2 mg/L (C4)] exposed chronically to Channa argus. After 14 and 28 days, we measured biochemical parameters, Cr (VI) accumulation, antioxidant activity and immune response in the serum, liver and gill, respectively. Our results shown that Cr (VI) accumulation will occur in certain tissues as the time of exposure in water prolongs, and the biochemical parameters in the serum will increase. Antioxidant enzymes (catalase, superoxide dismutase and glutathione peroxidase) gradually decreased with the increasing time of Cr (VI) concentration, while the malondialdehyde concentration increases. In addition, in the immune response, cortisol, aminotransferase (ALT), aspartate aminotransferases (AST), alkaline phosphatase (AKP), immunoglobulin M increase, but lysozyme decrease. These results indicated that exposure to waterborne Cr (VI) can exert adverse effects in C. argus by inducing Cr (VI) accumulation, immune responses and oxidative stress.

Download Full-text

Effects of Pristane Alone or Combined with Chloroquine on Macrophage Activation, Oxidative Stress, and Th1/Th2 Skewness

Journal of Immunology Research ◽

10.1155/2014/613136 ◽

2014 ◽

Vol 2014 ◽

pp. 1-9 ◽

Cited By ~ 7

Author(s):

Qiufang Ouyang ◽

Ziyang Huang ◽

Zhenhua Wang ◽

Xiaoqing Chen ◽

Jingqin Ni ◽

...

Keyword(s):

Oxidative Stress ◽

Biochemical Parameters ◽

Macrophage Activation Syndrome ◽

Macrophage Activation ◽

Lactic Dehydrogenase ◽

Peritoneal Macrophages ◽

Protective Role ◽

Hematological And Biochemical Parameters

We investigated the protective role of chloroquine against pristane-induced macrophage activation, oxidative stress, and Th1/Th2 skewness in C57BL/6J mice. Those mice were treated with pristane alone or combined with chloroquine. Hematological and biochemical parameters, macrophage phagocytic function, the oxidant/antioxidant index, cytokine for IFN-γ, TNF-α, IL-4, and IL-6, and the isotypes of IgG2a and IgG1 were determined. And the expression of T-bet/GATA-3 and IL-12/IL-10 mRNA in spleen were analyzed by real-time PCR. We found that pristane treatment for a period of 12 or 24 weeks triggered macrophage activation syndrome, characterized by hemophagocytosis in spleen and peripheral blood, enhanced lipid phagocytosis by peritoneal macrophages in vitro, erythropenia and leucopenia, increased anti-Smith, lactic dehydrogenase, triglyceride, and ferritin, as well as hypercytokinemia of IFN-γ, TNF-α, IL-4, and IL-6. In parallel, a significant increase in lipid peroxidation and a decrease in superoxide dismutase, glutathione, and catalase activity, as well as a skewed Th1/Th2 balance in spleen, were observed. However, chloroquine supplementation showed a remarkable amelioration of these abnormalities. Our data indicate that pristane administration induces macrophage activation, oxidative stress, and Th1/Th2 skewness, which can be attenuated by chloroquine.

Download Full-text