scholarly journals Lemon juice antioxidant activity against oxidative stress

2020 ◽  
Vol 17 (1(Suppl.)) ◽  
pp. 0207
Author(s):  
Safaa Ali
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Drinking Water ◽  
Total Protein ◽  
Blood Cells ◽  
Biochemical Parameters ◽  
Lemon Juice ◽  
Mean Corpuscular Hemoglobin ◽  
Female Mice ◽  
Hematological And Biochemical Parameters

This study is conducted to evaluate the therapeutic and antioxidant effect of lemon juice on some hematological and biochemical parameters. Thirty female mice used in this study were exposed to oxidative stress through giving them hydrogen peroxide in drinking water for 30 days. Animals randomly distributed over 3 groups, each group contained 10 animals and treated as follows: T1 control group (drinking distilled water only), T2 (0.75% hydrogen peroxide in drinking water) and T3 (0.75% hydrogen peroxide in drinking water with daily drenching with 1 mL lemon juice). At the end of the experiment, blood samples were collected from animals for evaluating the following hematological and biochemical parameters: Haemoglobin concentration (Hb), red blood cells count (RBC), white blood cells count (WBC), packed cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), platelet count (PLT), level of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and total protein. The results showed that T3 exhibited an enhancement in RBC count, Hb concentration, WBC, lymphocyte and total protein and reduction in the level of AST and ALT compared to T2. These findings clearly revealed the advance protective and antioxidant features of lemon juice on hematological and biochemical parameters of the oxidatively stressed female mice.

scholarly journals HEMATOLOGICAL AND BIOCHEMICAL BLOOD INDICES OF RAINBOW TROUT DEPENDING ON THE LEVEL OF PROTEIN IN FEEDS

2021 ◽  
Vol 12 (1) ◽  
pp. 5-13
Author(s):  
V. M. Kondratyuk
Keyword(s):  
Rainbow Trout ◽  
Protein Content ◽  
Total Protein ◽  
Blood Cells ◽  
Phagocytic Activity ◽  
Biochemical Parameters ◽  
Crude Protein ◽  
Crude Protein Content ◽  
Protein Nutrition ◽  
Hematological And Biochemical Parameters

The article considers the influence of compound feeds with different protein levels on hematological and biochemical parameters of trout blood. The purpose of the experiment was to establish the effect of different protein nutrition levels in commercial rainbow trout feeding on hematological and biochemical parameters of their blood. For this purpose, five experimental groups were formed by the method of analogs. The experiment lasted 210 days and was divided into two periods: equalization (10 days) and main (200 days). During the equalization period, the feeding ration was the same for fish in the control and experimental groups. In the main period, the level of protein in experimental feeds for different experimental trout groups ranged from 44 to 52 % per 1 kg. It was found that different levels of rainbow trout’s protein nutrition do not cause significant changes in blood morphological composition. It is proved that rearing trout using compound feeds with a crude protein content of 50 % and 52 % promotes the increase of red blood cells, hemoglobin, white blood cells and phagocytic activity in blood. The use of 44 % and 46 % low crude protein diets in fish feeding reduces the morphological components in blood. As a result of studies on the phagocytic activity of neutrophils in fish blood, a slight increase was revealed under the influence of the studied factor. The analysis of the obtained results showed that with increasing levels of crude protein in the diets of rainbow trout, the concentration of total protein and its fractions in blood serum increased. Accordingly, the amount of total protein in blood of fish in the control group was 60.0 g/L, in the experimental groups – it was within the range of 59.6–65.3 g/L. The increase in the level of rainbow trout’s protein nutrition in the experimental groups 4 and 5 caused an increase in the number of albumins, β-globulins and γ-globulins compared to control. As a result of experimental studies, an increase in the mineral status of rainbow trout blood during use of feeds with the crude protein content of 50–52 % was established. No differences were found during the assessment of trout white blood cell counts.

Effect of Turmeric and Ginger on Lipid Profile in Male Rats Exposed to Oxidative Stress

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Eman A. Al-Rekabi ◽  
Dheyaa K. Alomer ◽  
Rana Talib Al-Muswie ◽  
Khalid G. Al-Fartosi
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Drinking Water ◽  
Lipid Profile ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Male Rats ◽  
Control Group ◽  
Very Low Density Lipoprotein ◽  
Low Density

The present study aimed to investigate the effect of turmeric and ginger on lipid profile of male rats exposed to oxidative stress induced by hydrogen peroxide H2O2 at a concentration of 1% given with consumed drinking water to male rats. Methods: 200 mg/kg from turmeric and ginger were used, and the animals were treatment for 30 days. Results: the results showed a significant increase in cholesterol, triglycerides, low density lipoprotein (LDL), very low density lipoprotein (VLDL), whereas it explained a significant decrease in high density lipoprotein (HDL) of male rats exposed to oxidative stress when compared with control group. the results showed a significant decrease in cholesterol, triglycerides, (LDL), (VLDL), whereas it explained a significant increase in (HDL) of rats treated with turmeric and ginger at dose 200 mg/kg when compared with male rats exposed to oxidative stress.

scholarly journals Effect of aspartame on biochemical and oxidative stress parameters in rat blood

2015 ◽  
Vol 67 (2) ◽  
pp. 535-545 ◽  
Author(s):  
Marko Prokic ◽  
Milica Paunovic ◽  
Milos Matic ◽  
Natasa Djordjevic ◽  
Branka Ognjanovic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Biochemical Parameters ◽  
White Blood Cells ◽  
Lipid Peroxides ◽  
Hdl Cholesterol ◽  
Albino Rats ◽  
Total Body Mass ◽  
Antioxidative Status

Aspartame (ASP) is one of the most widely used nonnutritive sweeteners. This study investigates the chronic effects of ASP on hematological and biochemical parameters, and its effects on the oxidative/antioxidative status in the red blood cells of Wistar albino rats. Rats were provided with ASP (40 mg/kg/daily for six weeks) in drinking water. Increased food and fluid intake was observed in the ASP-treated rats. Total body mass was significantly decreased in the ASP-treated rats. Treatment with ASP caused an increase in the concentrations of glucose, cholesterol, LDL-cholesterol, and in the activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH), as well as a decrease in the levels of HDL-cholesterol in the serum. A significant decline in the number of white blood cells (WBC) was observed after ASP uptake. Based on the results we conclude that ASP induces oxidative stress, observed as an alteration of the glutathione redox status, which leads to increased concentrations of nitric oxide (NO) and lipid peroxides (LPO) in the red blood cells. Changes in biochemical parameters, lipid metabolism, as well as changes in the levels of oxidative stress markers and the appearance of signs of liver damage indicate that chronic use of ASP can lead to the development of hyperglycemia, hypercholesterolemia and associated diseases.

scholarly journals Study on Oxidative Metabolic Changes to Differentiate Exudative from Transudative Pleural Effusions

1970 ◽  
Vol 1 (1) ◽  
pp. 38-43
Author(s):  
V Narsimha Reddy ◽  
V Anil Kumar ◽  
M Srinivas ◽  
V Narayana Reddy
Keyword(s):  
Oxidative Stress ◽  
Lactate Dehydrogenase ◽  
Pleural Fluid ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Oxygen Species ◽  
Pleural Effusions ◽  
Glucose Levels ◽  
Protein Serum ◽  
Serum Ratio

The purpose of this present study was to differentiate transudates and exudates in pleural effusions. Oxidative stress has been associated with various respiratory disorders. Ninety patients of pleural effusions of diverse etiologies were participated in this study. Subjects underwent diagnostic thoracentesis and standard biochemical parameters (total protein, lactate dehydrogenase, glucose, MDA levels) were measured in pleural fluid and serum. MDA, total protein, lactate dehydrogenase (LDH), glucose levels in plural fluid were higher in exudates compared to transudates (p < 0.001). Total protein pleural fluid/ total protein serum ratio, LDH pleural fluid/LDH serum ratio and MDA pleural fluid/MDA serum ratio were raised in exudates compared to transudates (p < 0.001). The present study showed that oxidative stress was more in exudates compared to transudates, probably due to the production of reactive oxygen species and it may serve as a marker for differentiation between transudates and exudates in clinical practice. Key Words: Exudates, Melondialdehyde, Oxidative Stress, Pleural Effusion, Transudates    doi:10.3329/sjps.v1i1.1806 S. J. Pharm. Sci. 1(1&2): 38-43

Influence of “Sneznik-1/79” Mineral Water on Anthropometric, Functional and Biochemical Parameters of Professional Basketball Players: Role of Oxidative Stress

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Dijana Lalovic ◽  
Aleksandra Vranic ◽  
Jovana Jeremic ◽  
Dejan Stanojevic ◽  
Bolevich Sergey ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Drinking Water ◽  
Biochemical Parameters ◽  
Mineral Water ◽  
Redox Status ◽  
Fluid Replacement ◽  
Electrolyte Balance ◽  
Basketball Players ◽  
Professional Basketball ◽  
Adequate Hydration

AbstractAdequate hydration represents the balance between the water intake and loss and has an unambiguous significance for public health and it is essential to sustain life. The changes in electrolyte balance which occur during and after training affect on athletes health and performance. Therefore, fluid replacement with adequate mineral composition is of utmost importance. The aim of the present study was to examine the influence of low mineral water from the well Sneznik-1/79 on anthropometric, functional, biochemical parameters and redox status of professional basketball players. In total, 17 male basketball players were included, during the pre-competitive mesocycle, and after the initial testing, they were randomly divided into two groups: group 1 – consumed the commercial drinking water for four weeks (n = 7), and group 2 – consumed water from the well Sneznik-1/79 for four weeks (n = 10).Determination of the anthropometric, functional, biochemical parameters and redox status was performed. Our results pointed out that consumption of mineral water from the well Sneznik is completely safe from the aspect of affecting various anthropometric, functional and biochemical parameters as well as systemic oxidative stress of professional athletes. In addition, existence of discretely better effects over commercial drinking water indicates that a long period of monitoring may certainly be of interest for further investigation.

A study on oxidative stress, hematological and biochemical parameters in copper smelter workers

Toxin Reviews ◽  
2017 ◽  
pp. 1-6 ◽  
Author(s):  
Mahin Alishahi ◽  
Ali Akbar Malekirad ◽  
Ali Mandegary ◽  
Mohammad Abdollahi ◽  
Abbas Mozaffari ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Biochemical Parameters ◽  
Copper Smelter ◽  
Hematological And Biochemical Parameters

scholarly journals Impact of Multi-metal Mixture Administration Through Drinking Water on the Gut Bacterial Microflora and Oxidative Stress Parameters in Male and Female Mice

2022 ◽  
Vol 16 (1) ◽  
pp. 1-8
Author(s):  
Priyanka Bist ◽  
◽  
Sangeeta Choudhary ◽  
Keyword(s):  
Oxidative Stress ◽  
Heavy Metals ◽  
Heavy Metal ◽  
Drinking Water ◽  
Bacterial Count ◽  
High Dose ◽  
Male And Female ◽  
Metal Mixture ◽  
Female Mice ◽  
Multiple Heavy Metals

Background: Heavy metal containing wastes reaches to the food chain either directly or indirectly. These ingested toxic elements manifest direct impact on the gut ecosystem and its overall functioning. The present study explores the alteration in mice gut bacteria on exposure to mixture of toxic heavy metals through drinking water. Methods: Twelve experimental groups of Swiss albino male and female mice were exposed to the metal mixture of varying concentrations. Profiling of gut bacterial flora was done by periodical collection of fecal samples via culture-based technique. Redox status of all experimental animals was analyzed in blood samples collected on the day 30. Results: In comparison to the controls, nearly a 10-fold decline in colony forming units/ml was observed at higher modal concentrations (50× & 100×) at the end of 15 days, but 100-fold reduced bacterial count was recorded following 30 days of dosing. Sex specific significant alteration in the bacteria count and diversity was also observed. Overall experimental results showed a heavy metal dose-dependent decline in bacterial count and loss in diversity. Disturbance in the oxidative stress markers was recorded in response to high dose of metal mixture. In group receiving 100× dose, malondialdehyde levels were increased in the erythrocytes (P<0.05), and all of the other antioxidant parameters were decreased (P<0.05), except for reduced glutathione in both male and female mice. Conclusion: The present work is the first report on the multiple heavy metals induced gut microbiota alterations and its correlation to oxidative stress.

Dynamic Mitochondrial Responses to Oxidative Stress in Primary AML Cells Involve Structural Damage and Repair Activity.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 4139-4139
Author(s):  
Myung-Geun Shin ◽  
Hye-Ran Kim ◽  
Hyeoung-Joon Kim ◽  
Mi-Ji Kim ◽  
Kook Hoon ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Mrna Expression ◽  
Blood Cells ◽  
Normal Blood ◽  
Mtdna Damage ◽  
Mtdna Mutations ◽  
Intracellular Ros ◽  
Repair Activity ◽  
Mtdna Repair

Abstract Mitochondrial DNA (mtDNA) is particularly susceptible to oxidative damage and mutations because of the high level of reactive oxygen species (ROS) generated and the inefficiency of the mtDNA repair system. The oxidative stress elicited by chronic inflammation increases the number of mtDNA mutations and might correlate with a cancerous status. We postulated that increased oxidative stress in primary AML cells might cause mtDNA damage, which can lead to mtDNA mutations, structural changes, perturbation of mtDNA repair and biogenesis. Many mutation and polymorphisms (a total of 606 mtDNA sequence variants) were identified from 48 matched AML bone marrow and buccal mucosa samples, and blood samples from 57 control subjects. There were profound alterations in the 303 poly C, 16184 poly C, and 514 CA repeats. The intracellular ROS generation of cells can be investigated using the 2′,7′-dichlorfluorescein-diacetate and flow cytometry. The results were expressed as mean fluorescence intensity (MFI). MFI in primary AML cells (4,435±709) was significantly higher than those in control blood cells (1,562±141) (P<0.05). After then, we checked mRNA expression of peroxisome proliferators activated receptor gamma coactivator-1α (PGC-1α), PGC-1-related coactivator (PRC) and nuclear respiratory factor 1 (NRF-1) because these have been identified as an important regulator of intracellular ROS level and crucial factors linking external stimuli to mitochondrial biogenesis. As compared to normal blood cells, about 2.0 fold increase in NRF-1 mRNA expression was noted in primary AML, whereas PGC-1α and PRC mRNA expression were not remarkably changed. The supercoiled and open relaxed forms of mtDNA reflect functional and damaged molecules. Thus, sensitive detection of the relaxed and total mtDNA from the same DNA templates should allow quantitative measurements on mtDNA damage, repair and copy number change in stressed cells (Nucleic Acids Res2007;35:1377–88). Primary AML cells and normal blood cells were exposed to sublethal concentration of hydrogen peroxide to study mtDNA damage and repair activity using real-time PCR quantification. When AML cells and normal blood cells were treated with 240uM hydrogen peroxide, an average of 2.1 and 2.2 fold decreases in mtDNA amplification of AML cells were detected in two mtDNA markers (HV2 and cytochrome b) after 1-h and 2-h exposure, respectively. The exposure time-dependent increase in mtDNA amplification due to increased proportion of open relaxed forms was observed in normal blood cells after 15 min to 2-h treatment of hydrogen peroxide. In conclusion, the high level of intracellular ROS in primary AML cells might cause mtDNA damage, which can lead to mtDNA mutations and increased mitochondrial biogenesis by NRF-1 activation. Although the limited repair capacity hypothesis has been validated experimentally in some experimental systems, this study showed elevated mtDNA repair activity compared to normal blood cells. Our data also support the possibility that NRF-1 targeting approach may aid in the treatment of AML.

scholarly journals Dietary Supplementation with Chitosan Oligosaccharides Alleviates Oxidative Stress in Rats Challenged with Hydrogen Peroxide

Animals ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 55 ◽  
Author(s):  
Ruixia Lan ◽  
Qingqing Chang ◽  
Lilong An ◽  
Zhihui Zhao
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Drinking Water ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Control Group ◽  
Sprague Dawley ◽  
Antioxidant Power ◽  
Chitosan Oligosaccharides ◽  
Sprague Dawley Rats

Oxidative stress is induced by excessive oxidative radicals, which directly react with biomolecules, and damage lipids, proteins and DNA, leading to cell or organ injury. Supplementation of antioxidants to animals can be an effective way to modulate the antioxidant system. Chitosan oligosaccharides (COS) are the degraded products of chitosan or chitin, which has strong antioxidant, anti-inflammatory, and immune-enhancing competency. Therefore, the current study was conducted to evaluate the hypothesis that dietary supplementation with COS alleviates the damage caused by oxidative stress in Sprague Dawley rats challenged with hydrogen peroxide (H2O2). The rats were randomly divided into three groups: CON, control group, in which rats were fed a basal diet with normal drinking water; AS, H2O2 group, in which rats were fed the basal diet and 0.1% H2O2 in the drinking water; ASC, AS + COS group, in which rats were fed the basal diet with 200 mg/kg COS, and with 0.1% H2O2 in the drinking water. In vitro, COS exhibited better radical scavenging capacity of 1, 1-diphenyl-2-picrylhydrazyl (DPPH), superoxide anion (O2−), H2O2, and ferric ion reducing antioxidant power (FRAP) than butylated hydroxy anisole (BHA). In vivo, dietary supplementation with COS alleviated the H2O2-induced oxidative damage, evidenced by comparatively increasing activity of SOD, CAT, GSH-Px, GSH, and T-AOC, and comparatively decreasing level of MDA in serum, liver, spleen, and kidney. COS also comparatively alleviated the H2O2-induced inflammation. In conclusion, COS supplementation reduced lipid peroxidation and restored antioxidant capacity in Sprague Dawley rats, which were challenged with H2O2.

Trigonella foenum-graecumprotection against deltamethrin-induced toxic effects on haematological, biochemical, and oxidative stress parameters in rats

2014 ◽  
Vol 92 (8) ◽  
pp. 679-685 ◽  
Author(s):  
Mohamed M. Abdel-Daim ◽  
Mabrouk A. Abd Eldaim ◽  
Mohamed M. Mahmoud
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Body Mass ◽  
Biochemical Parameters ◽  
Toxic Effects ◽  
Protective Agent ◽  
Dependent Manner ◽  
Hematological And Biochemical Parameters ◽  
Trigonella Foenum Graecum L ◽  
And Oxidative Stress

Trigonella foenum-graecum L. is enriched with many active ingredients. TFG oil was evaluated for its protective effect against deltamethrin toxicity in rats. Rats of the control group were administered saline. The 2nd group was administered deltamethrin (DLM) orally at a concentration of 15 mg/kg body mass. The 3rd and 4th groups were administered DLM at a concentration of 15 mg/kg body mass and were fed diets containing 2.5% and 5% TFG oil, respectively. DLM intoxication reduced red blood cell and platelet counts, hemoglobin concentration, and hematocrit value while it induced leucocytosis. Furthermore, it increased serum levels of lactate dehydrogenase, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, γ-glutamyltransferase, triglycerides, cholesterol, uric acid, urea, and creatinine; increased hepatic, renal, and brain lipid peroxidation; decreased serum acetylcholine esterase level; and decreased hepatic, renal, and brain antioxidant markers’ activities. However, TFG oil kept the studied hematological and biochemical parameters within normal ranges. In addition, it prevented lipid peroxidation and oxidative stress induced by DLM intoxication in a dose-dependent manner. Therefore, these results indicated that TFG oil inhibited the toxic effects of DLM on hematological and biochemical parameters as well as oxidative status by its free radical scavenging and potent antioxidant activities, and it appeared to be a promising protective agent against DLM-induced toxicity.

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