Discovery of the Long Interspersed Nuclear Element-1 activation product [Open Reading Frame-1 (ORF1) protein] in human blood

2018 ◽  
Vol 487 ◽  
pp. 228-232 ◽  
Author(s):  
Keivan Hosseinnejad ◽  
Tyler Yin ◽  
Jeremy T. Gaskins ◽  
James L. Bailen ◽  
Saeed A. Jortani
Keyword(s):  
Human Blood ◽  
Open Reading Frame ◽  
Activation Product ◽  
Reading Frame ◽  
Orf1 Protein ◽  
Long Interspersed Nuclear Element ◽  
Open Reading Frame 1

scholarly journals Quasispecies of TT Virus (TTV) with Sequence Divergence in Hypervariable Regions of the Capsid Protein in Chronic TTV Infection

1999 ◽  
Vol 73 (11) ◽  
pp. 9604-9608 ◽  
Author(s):  
Tsutomu Nishizawa ◽  
Hiroaki Okamoto ◽  
Fumio Tsuda ◽  
Tatsuya Aikawa ◽  
Yoshiki Sugai ◽  
...  
Keyword(s):  
Amino Acids ◽  
Capsid Protein ◽  
Immune Surveillance ◽  
Sequence Divergence ◽  
Open Reading Frame ◽  
Amino Acid Substitutions ◽  
Reading Frame ◽  
Tt Virus ◽  
Hypervariable Regions ◽  
Open Reading Frame 1

ABSTRACT Three hypervariable regions were identified in a central portion of open reading frame 1 of TT virus DNA, which codes for a putative capsid protein of 770 amino acids. TT virus circulates as quasispecies, with many amino acid substitutions in hypervariable regions, to evade immune surveillance of the hosts and to establish a persistent infection.

DNA vaccination of pigs with open reading frame 1–7 of PRRS virus

Vaccine ◽  
2004 ◽  
Vol 22 (27-28) ◽  
pp. 3628-3641 ◽  
Author(s):  
Annette Malene Barfoed ◽  
Merete Blixenkrone-Møller ◽  
Merethe Holm Jensen ◽  
Anette Bøtner ◽  
Søren Kamstrup
Keyword(s):  
Dna Vaccination ◽  
Open Reading Frame ◽  
Reading Frame ◽  
Prrs Virus ◽  
Open Reading Frame 1

The open reading frame 1-encoded (‘36K’) protein of Carnation Italian ringspot virus localizes to mitochondria

2001 ◽  
Vol 82 (1) ◽  
pp. 29-34 ◽  
Author(s):  
L. Rubino ◽  
F. Weber-Lotfi ◽  
A. Dietrich ◽  
C. Stussi-Garaud ◽  
M. Russo
Keyword(s):  
Outer Membrane ◽  
Fluorescent Protein ◽  
Amorphous Material ◽  
Subcellular Fractionation ◽  
Open Reading Frame ◽  
Ringspot Virus ◽  
Mitochondrial Outer Membrane ◽  
Intermembrane Space ◽  
Reading Frame ◽  
Open Reading Frame 1

The localization of the 36 kDa (‘36K’) protein encoded by open reading frame 1 of Carnation Italian ringspot virus was studied in infected cells and in cells transiently expressing the 36K protein fused to green fluorescent protein (GFP). Subcellular fractionation demonstrated that the 36K protein accumulated in fractions containing mostly mitochondria. Fluorescence microscopy of transiently transformed cells showed that the 36K–GFP fusion protein accumulated in structures which could be stained with the mitochondrial-specific dye MitoTracker. However, these structures were larger than normal mitochondria and were irregular in shape and distribution in the cytoplasm. Electron microscopy showed severe alterations of mitochondria, which were often clumped. The stroma was more electron-opaque, the cristae were irregularly shaped, the intermembrane space was enlarged and the outer membrane was covered with an electron-dense amorphous material whose nature could not be determined. The organelle-targeted 36K protein seems to promote the overgrowth of the mitochondrial outer membrane.

Development of a Self-Cloning System for Actinomadura verrucosospora and Identification of Polyketide Synthase Genes Essential for Production of the Angucyclic Antibiotic Pradimicin

1999 ◽  
Vol 65 (6) ◽  
pp. 2703-2709 ◽  
Author(s):  
Tohru Dairi ◽  
Yoshimitsu Hamano ◽  
Tamotsu Furumai ◽  
Toshikazu Oki
Keyword(s):  
Nucleotide Sequence ◽  
Polyketide Synthase ◽  
Selectable Marker ◽  
Plasmid Vector ◽  
Open Reading Frame ◽  
Antibiotic Biosynthesis ◽  
Nucleotide Sequence Analysis ◽  
Reading Frame ◽  
Cloning System ◽  
Open Reading Frame 1

ABSTRACT A self-cloning system for Actinomadura verrucosospora, a producer of the angucyclic antibiotic pradimicin A (PRM A), has been developed. The system is based on reproducible and reliable protoplasting and regeneration conditions for A. verrucosospora and a novel plasmid vector that consists of a replicon from a newly found Actinomadura plasmid and a selectable marker cloned from the Actinomadurastrain. The system has an efficiency of more than 105CFU/microgram of DNA. Using this system, we have cloned and identified the polyketide synthase (PKS) genes essential for PRM A biosynthesis from A. verrucosospora. Nucleotide sequence analysis of the 3.5-kb SalI-SphI fragment showed that ketosynthase subunits (open reading frame 1 [ORF1] and ORF2) of the essential PKS genes have strong similarities (59 to 89%) to those for angucyclic antibiotic biosynthesis.

Correlation of long interspersed element-1 open reading frame 1 and c-Met proto-oncogene protein expression in ovarian cancer

2019 ◽  
Vol 41 (11) ◽  
pp. 1293-1299 ◽  
Author(s):  
Eun-Ji Ko ◽  
Young Lim Oh ◽  
Heung Yeol Kim ◽  
Wan Kyu Eo ◽  
Hongbae Kim ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Protein Expression ◽  
Open Reading Frame ◽  
Reading Frame ◽  
Long Interspersed Element ◽  
Oncogene Protein ◽  
Open Reading Frame 1

scholarly journals Differential translational initiation of lbp mRNA is caused by a 5′ upstream open reading frame 1

FEBS Letters ◽  
1997 ◽  
Vol 411 (2-3) ◽  
pp. 245-250 ◽  
Author(s):  
Maria Mittag ◽  
Christoph Eckerskorn ◽  
Kerstin Strupat ◽  
J.Woodland Hastings
Keyword(s):  
Open Reading Frame ◽  
Upstream Open Reading Frame ◽  
Translational Initiation ◽  
Reading Frame ◽  
Open Reading Frame 1

scholarly journals RNA 5′-Triphosphatase Activity of the Hepatitis E Virus Helicase Domain

2010 ◽  
Vol 84 (18) ◽  
pp. 9637-9641 ◽  
Author(s):  
Yogesh A. Karpe ◽  
Kavita S. Lole
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Open Reading Frame ◽  
Helicase Domain ◽  
Reading Frame ◽  
Essential Step ◽  
Positive Sense ◽  
Rna Triphosphatase ◽  
Rna Duplexes ◽  
Open Reading Frame 1

ABSTRACT Hepatitis E virus (HEV) has a positive-sense RNA genome with a 5′-m7G cap. HEV open reading frame 1 (ORF1) encodes a polyprotein with multiple enzyme domains required for replication. HEV helicase is a nucleoside triphosphatase (NTPase) with the ability to unwind RNA duplexes in the 5′-to-3′ direction. When incubated with 5′-[γ-32P]RNA and 5′-[α-32P]RNA, HEV helicase released 32P only from 5′-[γ-32P]RNA, showing specificity for the γ-β-triphosphate bond. Removal of γ-phosphate from the 5′ end of the primary transcripts (pppRNA to ppRNA) by RNA triphosphatase is an essential step during cap formation. It is suggested that HEV employs the helicase to mediate the first step of 5′ cap synthesis.

scholarly journals Translation Termination Reinitiation between Open Reading Frame 1 (ORF1) and ORF2 Enables Capsid Expression in a Bovine Norovirus without the Need for Production of Viral Subgenomic RNA

2008 ◽  
Vol 82 (17) ◽  
pp. 8917-8921 ◽  
Author(s):  
Christopher J. McCormick ◽  
Omar Salim ◽  
Paul R. Lambden ◽  
Ian N. Clarke
Keyword(s):  
Hepg2 Cells ◽  
Nonstructural Protein ◽  
Translation Termination ◽  
Surrogate Marker ◽  
Open Reading Frames ◽  
Open Reading Frame ◽  
Subgenomic Rna ◽  
Reading Frame ◽  
Reading Frames ◽  
Open Reading Frame 1

ABSTRACT A generally accepted view of norovirus replication is that capsid expression requires production of a subgenomic transcript, the presence of capsid often being used as a surrogate marker to indicate the occurrence of viral replication. Using a polymerase II-based baculovirus delivery system, we observed capsid expression following introduction of a full-length genogroup 3 norovirus genome into HepG2 cells. However, capsid expression occurred as a result of a novel translation termination/reinitiation event between the nonstructural-protein and capsid open reading frames, a feature that may be unique to genogroup 3 noroviruses.

Sign in / Sign up

Export Citation Format

Share Document