Vitamin B12 screening with holo-transcobalamin is more sensitive than total vitamin B12 screening

2019 ◽  
Vol 493 ◽  
pp. S404-S405
Author(s):  
M. Carta ◽  
D. Giavarina
Keyword(s):  
Vitamin B12 ◽  
Total Vitamin ◽  
Vitamin B12 Screening

THE CONSTITUENTS OF COD LIVER WITH VITAMIN B12 ACTIVITY FOR LACTOBACILLUS LEICHMANNII

1956 ◽  
Vol 34 (2) ◽  
pp. 191-196
Author(s):  
Beryl Truscott ◽  
P. L. Hoogland
Keyword(s):  
Ion Exchange ◽  
Vitamin B12 ◽  
Microbiological Assay ◽  
Countercurrent Distribution ◽  
Vitamin B ◽  
Partition Chromatography ◽  
Total Vitamin ◽  
Beef Liver ◽  
Fractionation Procedure ◽  
Lactobacillus Leichmannii

The identity of the compounds that together form the total microbiological vitamin B12 activity of cod-liver residue was determined by a fractionation procedure involving ion exchange, countercurrent distribution, partition chromatography, and microbiological assay. The presence of cyanocobalamin, hydroxocobalamin, and the desoxyribosides of thymine, uracil, hypoxanthine, xanthine, and guanine was demonstrated. The total vitamin B12 activity, as determined by microbiological assay with Lactobacillus leichmannii, and its distribution in cod-liver residue, were very similar to those of beef liver. Cod-liver residues may be used to replace beef liver in vitamin B12 preparations.

scholarly journals Estimating the Serum Folate Concentration Associated With the Red Blood Cell Folate Threshold for Optimal Neural Tube Defects Prevention: A Population Based Biomarker Survey

2021 ◽  
Vol 5 (Supplement_2) ◽  
pp. 644-644
Author(s):  
Amy Fothergill ◽  
Charles Rose ◽  
Krista Crider ◽  
Beena Bose ◽  
Heather Guetterman ◽  
...  
Keyword(s):  
Blood Cell ◽  
Vitamin B12 ◽  
Neural Tube ◽  
Red Blood Cell ◽  
Vitamin B12 Deficiency ◽  
Population Based ◽  
World Health ◽  
Serum Folate ◽  
Total Vitamin ◽  
B12 Deficiency

Abstract Objectives To estimate the serum folate insufficiency threshold (sf-IT) corresponding to the red blood cell (RBC) folate insufficiency threshold for optimal neural tube defect (NTD) prevention. Methods Participants were 977 women of reproductive age (WRA; 15–40y; not pregnant or lactating) from a population-based biomarker survey in Southern India. Venous blood samples were collected at enrollment. Plasma, serum, and red blood cells were centrifuged, processed, and stored < -80°C until batch analysis. Total vitamin B12 concentrations were measured via chemiluminescence; RBC and serum folate concentrations were measured using the World Health Organization-recommended microbiological assay. Vitamin B12 deficiency was defined as total vitamin B12 < 148 pmol/L. Folate insufficiency was defined as RBC folate < 748 nmol/L, the recommended calibrator-adjusted equivalent of the threshold for population optimal NTD prevention. A previously developed Bayesian model and the RBC and serum folate distributions in this population were used to estimate the sf-IT corresponding to the RBC folate insufficiency threshold for optimal NTD prevention, overall and by age, body mass index (BMI) category, HbA1c, anemia, and vitamin B12 status. Results The overall estimated median sf-IT was 37.8 nmol/L (95% credible interval [33.8–43.3]). This threshold was lower in overweight WRA (BMI: ≥25.0 kg/m2: 32.0 nmol/L [27.3–40.2] vs. BMI < 25.0 kg/m2: 36.2 nmol/L [32.2–43.3]), and varied by age (< 25y: 61.3 nmol/L [44.3–111.8]; 25 to 35y: 35.7 nmol/L [30.8–43.5]; ≥35y: 30.8 nmol/L [26.9–37.2]). The sf-IT was lower in anemic WRA (32.9 nmol/L [28.5–40.1]) compared to non-anemic WRA (42.0 nmol/L [36.1–51.3]), and lower in WRA with elevated HbA1c (≥5.7% to < 6.5: 32.4 nmol/L [27.3–41.6]; ≥6.5%: 20.9 nmol/L [17.8–25.6]) vs. WRA with HbA1c < 5.7% (43.8 nmol/L, [37.5–53.7]). The median sf-IT was higher in WRA with vitamin B12 deficiency (72.1 nmol/L [52.0–126.0]), compared to women who were not vitamin B12 deficient (28.1 nmol/L [25.6–31.5]). Conclusions The estimated sf-IT is dependent on anemia, elevated HbA1c, BMI, age, and vitamin B12 status. Funding Sources Centers for Disease Control and Prevention; AF was supported by the National Institutes of Health #5 T32 HD087137.

scholarly journals Cross-sectional Association Between Vitamin B12 Status and Probable Postpartum Depression in Indian Women

2020 ◽  
Author(s):  
Pooja Dhiman ◽  
Raji R Pillai ◽  
Anand B Wilson ◽  
Nancy Premkumar ◽  
Balaji Bharadwaj ◽  
...  
Keyword(s):  
Depressive Symptoms ◽  
Postpartum Depression ◽  
Vitamin B12 ◽  
Depression Scale ◽  
Biologically Active ◽  
Total Vitamin ◽  
Active Molecule ◽  
Cross Sectional ◽  
Indian Women ◽  
Probable Depression

Abstract BackgroundVitamin B12 is an essential micronutrient for neurological function, as it leads to the regeneration of methionine from homocysteine, which is precursor of biologically active molecule SAM. Pregnancy is a state of increased demand and delayed postpartum repletion of nutrients may predispose women to depression. MethodsWomen attending postpartum clinic at 6 weeks post-delivery were screened for the presence of depressive symptoms using Edinburg postpartum depression scale (EPDS). Based on a cut-off of 10, two groups were taken- women with probable depression with EPDS score>10 (n=217) and women without probable depression with EPDS score<10(n=217). Plasma total vitamin B12, holotranscobalamin, homocysteine, MMA, 5-methyl THF, SAM and serotonin levels were estimated using commercially available ELISA kits. Combined B12(cB12) score was calculated and analysis of resulting data was done using SPSS ver20.0. ResultsTotal vitamin B12 was significantly lower (p=0.001) and MMA (p=0.002) and 5-methyl THF(p<0.001) levels were higher in women with probable depression than women without probable depression. Combined B12 index was also lower in women with probable PPD (p=0.001). Women in the lowest vitamin B12 quartile had 4.53 times higher likelihood of probable postpartum depression (p < 0.001). Decreasing vitamin B12 (OR = 0.394; 95% CI: 0.189-0.822) and cB12 (OR=0.293; 95% CI:0182-0.470) and increasing MMA (OR = 2.14; 95% CI: 1.63-2.83) and 5-methyl THF levels (OR = 3.29; 95% CI: 1.59-6.83) were significantly associated with the risk of PPD.ConclusionLow vitamin B12 may contribute to depressive symptoms in vulnerable postpartum period.

scholarly journals Longitudinal Concentrations of Vitamin B12 and Vitamin B12-binding Proteins during Uncomplicated Pregnancy

2002 ◽  
Vol 48 (6) ◽  
pp. 928-933 ◽  
Author(s):  
Corinna Koebnick ◽  
Ulrike A Heins ◽  
Pieter C Dagnelie ◽  
Sunitha N Wickramasinghe ◽  
Indrika D Ratnayaka ◽  
...  
Keyword(s):  
Vitamin B12 ◽  
Pregnant Women ◽  
Reference Values ◽  
Binding Proteins ◽  
Daily Intake ◽  
Serum Vitamin ◽  
Total Vitamin ◽  
Uncomplicated Pregnancy ◽  
Biochemical Indices ◽  
Rbc Count

Abstract Background: Because reference values for vitamin B12 concentrations and vitamin B12-binding capacities for pregnant women have not been established, the reference values for nonpregnant women are often applied to assess vitamin B12 status. The aim of the present study was to describe ranges of biochemical indices of vitamin B12 status, including red blood cell (RBC) vitamin B12, saturated and unsaturated cobalamin-binding proteins, and binding capacities in all trimesters of uncomplicated pregnancy. Methods: A total of 39 healthy pregnant women with long-term daily intake of vitamin B12 &gt;2.6 μg/day and uncomplicated pregnancies participated in the study throughout their pregnancies. RBCs and serum vitamin B12, holo-haptocorrin, unsaturated cobalamin-binding proteins, unsaturated and total vitamin B12-binding capacities, total homocysteine (tHcy), and RBC count were assessed in weeks 9–12, 20–22, and 36–38 of gestation. Results: Significant changes in vitamin B12 status occurred in the course of pregnancy. Serum vitamin B12 concentrations and percentage of saturation of vitamin B12-binding proteins decreased steadily throughout pregnancy. In the third trimester, 35% of the participants had serum vitamin B12 concentrations &lt;150 pmol/L and 68.6% had &lt;15% saturation of total vitamin B12-binding capacities, but no women had RBC vitamin B12 concentrations &lt;148 pmol/L. However, the decrease in these indices was not associated with reduced hemoglobin concentrations or RBC count or with increased tHcy concentrations. Conclusions: Our findings suggest that the reference values for vitamin B12 status in nonpregnant women may not be applicable to pregnant women.

scholarly journals The epidemiology and importance of vitamin B12 screening in diabetic patients

2021 ◽  
Author(s):  
Abrar A. A. Yamani ◽  
Jameel A. Awadain ◽  
Yousif A. A. Saleh ◽  
Mohammad S. Baothman ◽  
Feras H. Alhussainy ◽  
...  
Keyword(s):  
Vitamin B12 ◽  
Clinical Manifestations ◽  
Vitamin B12 Deficiency ◽  
Diabetic Patients ◽  
Type I ◽  
Type Ii ◽  
Impaired Metabolism ◽  
B12 Deficiency ◽  
Vitamin B12 Screening

Peripheral neuropathy is a commonly reported chronic adverse event among diabetes mellitus (DM) patients secondary to poor glycemic control. It might also result secondary to deficiency of vitamin B12, reportedly common among diabetic patients. Deficiency of vitamin B12 might result from prolonged metformin administration in patients with type II DM (T2DM). It might also result from reduced absorption and impaired metabolism-related events in type I DM (T1DM) patients. This occurs secondary to the presence of associated autoimmune disorders. Vitamin B12 deficiency is a commonly encountered condition among diabetic patients, both T1DM and T2DM, with variable etiologies. Our current study discussed the epidemiology and importance of screening of vitamin B12 in these patients. However, our findings show that screening is not commonly practiced in different settings. Therefore, awareness is low about the benefits and complications of this practice. Therefore, further research is encouraged to alleviate the quality of care in diabetic patients. Screening for vitamin B12 deficiency might intervene against any potential complications, including irreversible, painful, and potentially disabling nerve injury. Accordingly, it is recommended that screening should be initiated since the start of metformin administration and every year or when relevant clinical manifestations were reported.

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