Ultra-thin temperature controllable microwell array chip for continuous real-time high-resolution imaging of living single cells

2021 ◽  
Author(s):  
Yuanyuan Wu ◽  
Lei Zhao ◽  
Yaran Chang ◽  
Liang Zhao ◽  
Guangsheng Guo ◽  
...  
Keyword(s):  
High Resolution ◽  
Real Time ◽  
Single Cells ◽  
High Resolution Imaging ◽  
Microwell Array ◽  
Resolution Imaging ◽  
Living Single

scholarly journals Tailoring exceptional points in a hybrid PT-symmetric and anti-PT-symmetric scattering system

Nanophotonics ◽  
2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Zhicheng Xiao ◽  
Andrea Alù
Keyword(s):  
High Resolution ◽  
Real Time ◽  
Quantum Systems ◽  
Symmetric System ◽  
Microwave Circuit ◽  
High Resolution Imaging ◽  
Fano Resonances ◽  
Real Time Control ◽  
Time Control ◽  
Resolution Imaging

Abstract Fano resonances feature an asymmetric lineshape with controllable linewidth, stemming from the interplay between bright and dark resonances. They provide efficient opportunities to shape the scattering lineshape, but they usually lack flexibility and tunability and are hindered by loss in passive systems. Here, we explore a hybrid parity-time (PT) and anti-parity-time (APT) symmetric system supporting unitary scattering features with highly tunable Fano resonances. The PT-APT-symmetric system can be envisioned in nanophotonic and microwave circuit implementations, allowing for real-time control of the scattering lineshape and its underlying singularities. Our study shows the opportunities enabled by non-Hermitian platforms to control scattering lineshapes for a plethora of photonic, electronic, and quantum systems, with potential for high-resolution imaging, switching, sensing, and multiplexing.

scholarly journals μCB-seq: Microfluidic cell barcoding and sequencing for high-resolution imaging and sequencing of single cells

2020 ◽  
Author(s):  
Tyler N. Chen ◽  
Anushka Gupta ◽  
Mansi Zalavadia ◽  
Aaron M. Streets
Keyword(s):  
High Resolution ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Protein Localization ◽  
Single Cells ◽  
Optical Measurements ◽  
Detection Sensitivity ◽  
High Resolution Imaging ◽  
Cell Analysis ◽  
Resolution Imaging

AbstractSingle-cell RNA sequencing (scRNA-seq) enables the investigation of complex biological processes in multicellular organisms with high resolution. However, many phenotypic features that are critical to understanding the functional role of cells in a heterogeneous tissue or organ are not directly encoded in the genome and therefore cannot be profiled with scRNA-seq. Quantitative optical microscopy has long been a powerful approach for characterizing diverse cellular phenotypes including cell morphology, protein localization, and chemical composition. Combining scRNA-seq with optical imaging has the potential to provide comprehensive single-cell analysis, allowing for functional integration of gene expression profiling and cell-state characterization. However, it is difficult to track single cells through both measurements; therefore, coupling current scRNA-seq protocols with optical measurements remains a challenge. Here, we report Microfluidic Cell Barcoding and Sequencing (μCB-seq), a microfluidic platform that combines high-resolution imaging and sequencing of single cells. μCB-seq is enabled by a novel fabrication method that preloads primers with known barcode sequences inside addressable reaction chambers of a microfluidic device. In addition to enabling multi-modal single-cell analysis, μCB-seq improves gene detection sensitivity, providing a scalable and accurate method for information-rich characterization of single cells.

View-expansive Microscope System with Real-time High-resolution Imaging for Simplified Microinjection Experiments

2021 ◽  
Author(s):  
Tadayoshi Aoyama ◽  
Sarau Takeno ◽  
Kazuki Hano ◽  
Masaki Takasu ◽  
Masaru Takeuchi ◽  
...  
Keyword(s):  
High Resolution ◽  
Real Time ◽  
High Resolution Imaging ◽  
Resolution Imaging

scholarly journals Rapid Diagnosis of Tuberculosis by Real-Time High-Resolution Imaging of Mycobacterium tuberculosis Colonies

2015 ◽  
Vol 53 (8) ◽  
pp. 2693-2696 ◽  
Author(s):  
Ramzi Ghodbane ◽  
Shady Asmar ◽  
Marlena Betzner ◽  
Marie Linet ◽  
Joseph Pierquin ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
High Resolution ◽  
Real Time ◽  
Solid Medium ◽  
Contamination Rate ◽  
High Resolution Imaging ◽  
Standard Protocol ◽  
Content Type ◽  
The Real ◽  
Resolution Imaging

Culture remains the cornerstone of diagnosis for pulmonary tuberculosis, but the fastidiousness ofMycobacterium tuberculosismay delay culture-based diagnosis for weeks. We evaluated the performance of real-time high-resolution imaging for the rapid detection ofM. tuberculosiscolonies growing on a solid medium. A total of 50 clinical specimens, including 42 sputum specimens, 4 stool specimens, 2 bronchoalveolar lavage fluid specimens, and 2 bronchial aspirate fluid specimens were prospectively inoculated into (i) a commercially available Middlebrook broth and evaluated for mycobacterial growth indirectly detected by measuring oxygen consumption (standard protocol) and (ii) a home-made solid medium incubated in an incubator featuring real-time high-resolution imaging of colonies (real-time protocol). Isolates were identified by Ziehl-Neelsen staining and matrix-assisted laser desorption ionization–time of flight mass spectrometry. Use of the standard protocol yielded 14/50 (28%)M. tuberculosisisolates, which is not significantly different from the 13/50 (26%)M. tuberculosisisolates found using the real-time protocol (P= 1.00 by Fisher's exact test), and the contamination rate of 1/50 (2%) was not significantly different from the contamination rate of 2/50 (4%) using the real-time protocol (P= 1.00). The real-time imaging protocol showed a 4.4-fold reduction in time to detection, 82 ± 54 h versus 360 ± 142 h (P< 0.05). These preliminary data give the proof of concept that real-time high-resolution imaging ofM. tuberculosiscolonies is a new technology that shortens the time to growth detection and the laboratory diagnosis of pulmonary tuberculosis.

scholarly journals μCB-seq: microfluidic cell barcoding and sequencing for high-resolution imaging and sequencing of single cells

Lab on a Chip ◽  
2020 ◽  
Vol 20 (21) ◽  
pp. 3899-3913
Author(s):  
Tyler N. Chen ◽  
Anushka Gupta ◽  
Mansi D. Zalavadia ◽  
Aaron Streets
Keyword(s):  
High Resolution ◽  
Single Cells ◽  
Genomic Analysis ◽  
High Resolution Imaging ◽  
Molecular Barcoding ◽  
Resolution Imaging ◽  
On Chip

We present a platform for on-chip molecular barcoding that combines high-resolution imaging with genomic analysis, enabling multi-modal phenotypic measurements in single cells.

Single shot real-time high-resolution imaging through dynamic turbid media based on deep learning

2022 ◽  
Vol 149 ◽  
pp. 106819
Author(s):  
Huazheng Wu ◽  
Xiangfeng Meng ◽  
Xiulun Yang ◽  
Xianye Li ◽  
Yongkai Yin
Keyword(s):  
Deep Learning ◽  
High Resolution ◽  
Real Time ◽  
Turbid Media ◽  
Single Shot ◽  
High Resolution Imaging ◽  
Resolution Imaging

Microwave Photonic Bistatic Radar for Real-Time and High-Resolution Imaging

2020 ◽  
Vol 32 (21) ◽  
pp. 1397-1400
Author(s):  
Beichen Fan ◽  
Fangzheng Zhang ◽  
Cong Ma ◽  
Yue Yang ◽  
Shilong Pan ◽  
...  
Keyword(s):  
High Resolution ◽  
Real Time ◽  
Bistatic Radar ◽  
High Resolution Imaging ◽  
Microwave Photonic ◽  
Resolution Imaging
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