Influence of age and sex on the spontaneous DNA damage detected by Micronucleus test and Comet assay in mice peripheral blood cells

2008 ◽  
Vol 32 (10) ◽  
pp. 1223-1229 ◽  
Author(s):  
V HEUSER ◽  
V DEANDRADE ◽  
A PERES ◽  
L GOMESDEMACEDOBRAGA ◽  
J BOGOCHIES
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Micronucleus Test ◽  
Peripheral Blood Cells ◽  
Age And Sex

scholarly journals Paracoccidioidomycosis: no genetic damage in human peripheral blood cells of patients assessed by single-cell gel (comet) assay

2007 ◽  
Vol 40 (4) ◽  
pp. 476-478 ◽  
Author(s):  
Renata Aparecida Martinez Antunes Ribeiro-Vieira ◽  
Daniel Araki Ribeiro ◽  
Daisy Maria Favero Salvadori ◽  
Sílvio Alencar Marques
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Human Peripheral Blood ◽  
Paracoccidioides Brasiliensis ◽  
Peripheral Blood Cells ◽  
Genetic Damage ◽  
Systemic Fungal Infection ◽  
Dna Breakage

Paracoccidioidomycosis is a systemic fungal infection caused by Paracoccidioides brasiliensis. As infectious diseases can cause DNA damage, the authors aimed at analyzing DNA breakage in peripheral blood cells of patients with paracoccidioidomycosis by using the comet assay. The results suggested that paracoccidioidomycosis does not cause genotoxicity.

scholarly journals In vivo genotoxicity assessment of aluminium oxide nanomaterials in rat peripheral blood cells using the comet assay and micronucleus test

Mutagenesis ◽  
2009 ◽  
Vol 24 (3) ◽  
pp. 245-251 ◽  
Author(s):  
A. Balasubramanyam ◽  
N. Sailaja ◽  
M. Mahboob ◽  
M. F. Rahman ◽  
S. M. Hussain ◽  
...  
Keyword(s):  
Comet Assay ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Micronucleus Test ◽  
Aluminium Oxide ◽  
Peripheral Blood Cells

scholarly journals Persistent Increased Frequency of Genomic Instability in Women Diagnosed with Breast Cancer: Before, during, and after Treatments

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Márcia Fernanda Correia Jardim Paz ◽  
André Luiz Pinho Sobral ◽  
Jaqueline Nascimento Picada ◽  
Ivana Grivicich ◽  
Antonio Luiz Gomes Júnior ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Dna Damage ◽  
Comet Assay ◽  
Peripheral Blood ◽  
Micronucleus Test ◽  
Cancer Control ◽  
Control Group ◽  
Breast Cancer Patients ◽  
Buccal Cells ◽  
Oncological Treatment

This study aimed to evaluate DNA damage in patients with breast cancer before treatment (background) and after chemotherapy (QT) and radiotherapy (RT) treatment using the Comet assay in peripheral blood and the micronucleus test in buccal cells. We also evaluated repair of DNA damage after the end of RT, as well as the response of patient’s cells before treatment with an oxidizing agent (H2O2; challenge assay). Fifty women with a mammographic diagnosis negative for cancer (control group) and 100 women with a diagnosis of breast cancer (followed up during the treatment) were involved in this study. The significant DNA damage was observed by increasing in the index and frequency of damage along with the increasing of the frequency of micronuclei in peripheral blood and cells of the buccal mucosa, respectively. Despite the variability of the responses of breast cancer patients, the individuals presented lesions on the DNA, detected by the Comet assay and micronucleus Test, from the diagnosis until the end of the oncological treatment and were more susceptible to oxidative stress. We can conclude that the damages were due to clastogenic and/or aneugenic effects related to the neoplasia itself and that they increased, especially after RT.

DNA Damage Signatures in Peripheral Blood Cells as Biomarkers in Prodromal Huntington's Disease

2018 ◽  
Author(s):  
Imma Castaldo ◽  
Mariarosaria De Rosa ◽  
Antonella Romano ◽  
Candida Zuchegna ◽  
Ferdinando Squitieri ◽  
...  
Keyword(s):  
Dna Damage ◽  
Huntington's Disease ◽  
Huntington’S Disease ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Peripheral Blood Cells

scholarly journals MODIFICATION OF THE TUMOR/INDUCED BYSTANDER EFFECT BY IRRADIATION UNDER COCULTIVATION OF LYMPHOCYTES FROM PATIENTS WITH CHRONIC LYMPHOCYTIC LEUKEMIA AND LYMPHOCYTES FROM HEALTHY DONORS

2021 ◽  
Vol 26 ◽  
pp. 248-259
Author(s):  
D. Кurinnyi ◽  
◽  
S. Rushkovsky ◽  
O. Demchenko ◽  
M. Romanenko ◽  
...  
Keyword(s):  
Dna Damage ◽  
Chronic Lymphocytic Leukemia ◽  
Ionizing Radiation ◽  
Comet Assay ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Bystander Effect ◽  
Peripheral Blood Lymphocytes ◽  
Lymphocytic Leukemia ◽  
Healthy Individuals

Objective: Study the tumor-induced bystander effect of blood cells from chronic lymphocytic leukemia (CLL) patients on non-transformed bystander cells (peripheral blood lymphocytes (PBL) of conditionally healthy individuals) and the possibility of its modification after the impact of ionizing radiation. Materials and methods. We carried out cocultivation and separate cultivation of blood samples from conditionally healthy volunteers and patients with CLL according to our technique. Using the Comet assay, the relative level of DNA damage was evaluated. Results. A statistically significant increase (р < 0.001) in the level of DNA damage in PBL culture of conditionally healthy individuals after co-cultivation with malignant cells of CLL patients was observed. After irradiation, a drop in the level of cells with a high degree of DNA damage was noted, which was connected with an increase in the frequency of cells that were delayed in division at the S stage of the cell cycle. An increase in apoptotic activity in cultures of bystander cells was observed in all variants of the experiment (р < 0.001). Conclusion. The influence of irradiated blood cells of patients with CLL results in an enhancement of the tumorinduced bystander effect manifestation in the PBL of conditionally healthy individuals. Key words: tumor-induced bystander effect, peripheral blood lymphocytes, Comet assay, ionizing radiation.

Levodopa therapy reduces DNA damage in peripheral blood cells of patients with Parkinson’s disease

2008 ◽  
Vol 25 (4) ◽  
pp. 321-330 ◽  
Author(s):  
T. Cornetta ◽  
S. Palma ◽  
I. Aprile ◽  
L. Padua ◽  
P. Tonali ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Dna Damage ◽  
Parkinson's Disease ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Levodopa Therapy ◽  
Peripheral Blood Cells
Sign in / Sign up

Export Citation Format

Share Document