Natural organic matter (NOM) in water reacts with chlorine or other disinfectants and form hazardous disinfectant by-products (DBPs). This study aimed to detect the presence of NOM in a conventional water distribution system using UV absorbance at 254 nm as a surrogate. Two water treatment plants were selected, namely, Jalan Baharu Water Treatment Plant (JBWTP) and Lubok Buntar Water Treatment Plant (LBWTP). Aside from determining the amount of NOM, the reduction of UV254after completing the series of treatments (coagulation, flocculation, sedimentation, filtration, and disinfection) was also observed. The presence of UV254in both raw water and treated water samples confirmed the presence of NOM. The concentration of UV254recorded at JBWTP and LBWTP were 0.14 and 0.13 cm−1, respectively. After the treatment processes, the concentration was reduced to 0.04 cm−1for JBWTP and 0.03 cm−1for LBWTP. These results indicated that the water supply in both plants contains DBP precursors and implied the possibility of DBP formation in the system. Moreover, the percentage reduction of UV254recorded were 69% and 75% for JBWTP and LBWTP, respectively.