scholarly journals Transcription factor proteomics: Identification by a novel gel mobility shift-three-dimensional electrophoresis method coupled with southwestern blot and high-performance liquid chromatography–electrospray-mass spectrometry analysis

2011 ◽  
Vol 1218 (39) ◽  
pp. 7003-7015 ◽  
Author(s):  
Daifeng Jiang ◽  
Yinshan Jia ◽  
Harry W. Jarrett
Keyword(s):  
Mass Spectrometry ◽  
High Performance ◽  
Electrospray Mass Spectrometry ◽  
Three Dimensional ◽  
Mass Spectrometry Analysis ◽  
Mobility Shift ◽  
Spectrometry Analysis ◽  
Electrophoresis Method ◽  
Gel Mobility Shift ◽  
Dimensional Electrophoresis

scholarly journals A straightforward gel-free proteomics pipeline assisted by liquid isoelectric focusing (OFFGEL) and mass spectrometry analysis to study bovine meat proteome

2020 ◽  
pp. 108201322092914
Author(s):  
Enrique Sentandreu ◽  
Claudia Fuente-García ◽  
José L Navarro ◽  
Miguel A Sentandreu
Keyword(s):  
Mass Spectrometry ◽  
Isoelectric Focusing ◽  
Quantitative Data ◽  
Ion Trap ◽  
Three Dimensional ◽  
Mass Spectrometry Analysis ◽  
Liquid Chromatography Mass Spectrometry ◽  
Protein Fractionation ◽  
Spectrometry Analysis ◽  
Proteomic Approach

Bovine sarcoplasmic sub-proteome was studied through a straightforward gel-free pipeline supported by liquid isoelectric focusing (OFFGEL) protein fractionation coupled to liquid chromatography-mass spectrometry (LC-MS) analysis. Full-MS and data-dependent MS/MS analyses were simultaneously performed by a conventional three-dimensional ion-trap addressing targeted quantitative and untargeted qualitative research, respectively. There were unambiguously identified 47 proteins distributed along 12 OFFGEL fractions assayed. Regarding intermediate- and high-abundant peptides, bulky quantitative data processing performed by MZmine 2 freeware yielded a satisfactory linearity and coefficient of variation with r2 in the 0.95–0.99 range and about 25%, respectively. Up to 41 peptides from 20 identified proteins were relatively quantified throughout OFFGEL fractions. This reliable, flexible and affordable gel-free proteomic approach could be readily implemented by industry to improve quality assessment of protein-based food products.

Electrospray mass spectrometry analysis of liquid-phase organic synthesis

1998 ◽  
Vol 4 (1) ◽  
pp. 233 ◽  
Author(s):  
F. Nativel ◽  
C. Enjalbal ◽  
F. Lamaty ◽  
R. Lazaro ◽  
J. Martinez ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Phase ◽  
Organic Synthesis ◽  
Electrospray Mass Spectrometry ◽  
Mass Spectrometry Analysis ◽  
Spectrometry Analysis
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