Deconvolution of overlapping spectral polymer signals in size exclusion separation-diode array detection separations by implementing a multivariate curve resolution method optimized by alternating least square

2014 ◽  
Vol 1342 ◽  
pp. 63-69 ◽  
Author(s):  
Tim Van Hoeylandt ◽  
Kai Chen ◽  
Filip Du Prez ◽  
Frédéric Lynen
Keyword(s):  
Least Square ◽  
Size Exclusion ◽  
Multivariate Curve Resolution ◽  
Diode Array ◽  
Diode Array Detection ◽  
Resolution Method ◽  
Curve Resolution ◽  
Array Detection

Fast chromatography of complex biocide mixtures using diode array detection and multivariate curve resolution

2004 ◽  
Vol 74 (2) ◽  
pp. 293-303 ◽  
Author(s):  
Emma Peré-Trepat ◽  
Alain Hildebrandt ◽  
Damià Barceló ◽  
Silvia Lacorte ◽  
Romà Tauler
Keyword(s):  
Multivariate Curve Resolution ◽  
Diode Array ◽  
Diode Array Detection ◽  
Curve Resolution ◽  
Array Detection

Application of Curve Resolution Algorithms in the Study Drug Photodegradation Kinetics—The Example of Moclobemide

2012 ◽  
Vol 95 (3) ◽  
pp. 708-712 ◽  
Author(s):  
Robert Skibiński ◽  
Łukasz Komsta
Keyword(s):  
Reference Method ◽  
Study Drug ◽  
Diode Array ◽  
Diode Array Detection ◽  
Concentration Profiles ◽  
Chemometric Method ◽  
Curve Resolution ◽  
Array Detection ◽  
Major Degradation Product ◽  
Good Agreement

Abstract The photodegradation of moclobemide was studied in methanolic media. Ultra-HPLC (UHPLC)/MS/MS analysis proved decomposition to 4-chlorobenzamide as a major degradation product and small amounts of Ro 16-3177 (4-chloro-N-[2-[(2-hydroxyethyl)amino] ethyl]benzamide) and 2-[(4-chlorobenzylidene)amino]-N-[2-ethoxyethenyl]ethenamine. The methanolic solution was investigated spectrophotometrically in the UV region, registering the spectra during 30 min of degradation. Using reference spectra and a multivariate chemometric method (multi- variate curve resolution-alternating least squares), the spectra were resolved and concentration profiles were obtained. The obtained results were in good agreement with a quantitative approach, with UHPLC- diode array detection as the reference method.

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