Liquid chromatography quadrupole-Orbitrap mass spectrometry for the simultaneous analysis of advanced glycation end products and protein-derived cross-links in food and biological matrices

2020 ◽  
Vol 1615 ◽  
pp. 460767 ◽  
Author(s):  
Mahesha M. Poojary ◽  
Wei Zhang ◽  
Ines Greco ◽  
Cristian De Gobba ◽  
Karsten Olsen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Advanced Glycation End Products ◽  
Simultaneous Analysis ◽  
Advanced Glycation ◽  
Biological Matrices ◽  
Orbitrap Mass Spectrometry ◽  
Glycation End Products ◽  
End Products ◽  
Cross Links

scholarly journals Ethyl Pyruvate Prevents Renal Damage Induced by Methylglyoxal-Derived Advanced Glycation End Products

2019 ◽  
Vol 2019 ◽  
pp. 1-9
Author(s):  
Eunsoo Jung ◽  
Wan Seok Kang ◽  
Kyuhyung Jo ◽  
Junghyun Kim
Keyword(s):  
Advanced Glycation End Products ◽  
Ethyl Pyruvate ◽  
Renal Diseases ◽  
Dependent Manner ◽  
Advanced Glycation ◽  
Glycation End Products ◽  
End Products ◽  
Cross Links

The renal accumulation of advanced glycation end products (AGEs) is a causative factor of various renal diseases, including chronic kidney disease and diabetic nephropathy. AGE inhibitors, such as aminoguanidine and pyridoxamine, have the therapeutic activities for reversing the increase in renal AGE burden. This study evaluated the inhibitory effects of ethyl pyruvate (EP) on methylglyoxal- (MGO-) modified AGE cross-links with proteins in vitro. We also determined the potential activity of EP in reducing the renal AGE burden in exogenously MGO-injected rats. EP inhibited MGO-modified AGE-bovine serum albumin (BSA) cross-links to collagen (IC50=0.19±0.03 mM) in a dose-dependent manner, and its activity was stronger than aminoguanidine (IC50=35.97±0.85 mM). In addition, EP directly trapped MGO (IC50=4.41±0.08 mM) in vitro. In exogenous MGO-injected rats, EP suppressed AGE burden and MGO-induced oxidative injury in renal tissues. These activities of EP on the MGO-mediated AGEs cross-links with protein in vitro and in vivo showed its pharmacological potential for inhibiting AGE-induced renal diseases.

Development of a method for the determination of advanced glycation end products precursors by liquid chromatography and its application in human urine samples

2012 ◽  
Vol 35 (19) ◽  
pp. 2575-2584 ◽  
Author(s):  
María del Carmen Hurtado-Sánchez ◽  
Anunciación Espinosa-Mansilla ◽  
María Isabel Rodríguez-Cáceres ◽  
Elisabet Martín-Tornero ◽  
Isabel Durán-Merás
Keyword(s):  
Liquid Chromatography ◽  
Advanced Glycation End Products ◽  
Human Urine ◽  
Urine Samples ◽  
Advanced Glycation ◽  
Human Urine Samples ◽  
Glycation End Products ◽  
End Products
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