Production and characterization of insoluble α-1,3-linked glucan and soluble α-1,6-linked dextran from Leuconostoc pseudomesenteroides G29

Author(s):  
Yiya Wang ◽  
Tao Sun ◽  
Yinzhu Wang ◽  
Hao Wu ◽  
Yan Fang ◽  
...  
PLoS ONE ◽  
2013 ◽  
Vol 8 (8) ◽  
pp. e70484 ◽  
Author(s):  
Kahina Maya Makhloufi ◽  
Alyssa Carré-Mlouka ◽  
Jean Peduzzi ◽  
Carine Lombard ◽  
Carol Ann van Reenen ◽  
...  

1999 ◽  
Vol 37 (12) ◽  
pp. 4124-4126 ◽  
Author(s):  
Elisabete A. Cappelli ◽  
Rosana R. Barros ◽  
Thereza Cristina F. Camello ◽  
Lúcia M. Teixeira ◽  
Vânia Lúcia C. Merquior

The phenotypic and genotypic characterization of five clinical isolates of Leuconostoc pseudomesenteroides associated with nosocomially acquired urinary tract infections is described. All the strains were susceptible to chloramphenicol, clindamycin, erythromycin, gentamicin, and tetracycline; all were resistant to nalidixic acid, norfloxacin, and vancomycin; and all were intermediately affected by ampicillin and penicillin. Analysis of chromosomal DNA by pulsed-field gel electrophoresis after treatment with SmaI indicated a clonal relationship of the isolates. The results provide evidence for the possibility of nosocomial transmission of this unusual opportunistic, vancomycin-resistant pathogen.


2019 ◽  
Vol 207 ◽  
pp. 218-223 ◽  
Author(s):  
Guangbin Ye ◽  
Genliang Li ◽  
Changli Wang ◽  
Bo Ling ◽  
Ruirui Yang ◽  
...  

2018 ◽  
Vol 107 ◽  
pp. 2234-2241 ◽  
Author(s):  
Qingqing Zhou ◽  
Fang Feng ◽  
Yanfang Yang ◽  
Fangkun Zhao ◽  
Renpeng Du ◽  
...  

2012 ◽  
Vol 84 (2) ◽  
pp. 495-508 ◽  
Author(s):  
Elinalva M. Paulo ◽  
Elisangela F. Boffo ◽  
Alexsandro Branco ◽  
Ângela M.M.P. Valente ◽  
Itamar S. Melo ◽  
...  

The genus Leuconostoc belongs to a group of lactic acid bacteria usually isolated from fermented vegetables, which includes species involved in the production of exopolysaccharides (EPS). These biopolymers possess considerable commercial potential. Because of the wide variety of industrial applications of EPS, this study aimed to produce and characterize the native exopolysaccharide strain Leuconostoc pseudomesenteroides R2, which was isolated from cabbage collected in a semi-arid region of Bahia. We employed the following conditions for the production of EPS: 10.7% sucrose, pH 8.2, without agitation and incubation at 28ºC for 30 hours. The fermentation broth was treated with ethanol and generated two types of polysaccharide substances (EPS I and EPS II). The identification of EPS I and EPS II was conducted using FT-IR, ¹H, 13C and DEPT-135 NMR spectra. The two substances were identified as linear dextran α polysaccharides (1 → 6) which indicated different characteristics with respect to thermal analysis and density of free packaging, viscosity and time of solubilization. Both dextrans are of low density, possess high thermal stability and exhibited the behavior characteristic of pseudoplastic polymers.


Author(s):  
B. L. Soloff ◽  
T. A. Rado

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.


Author(s):  
A.R. Pelton ◽  
A.F. Marshall ◽  
Y.S. Lee

Amorphous materials are of current interest due to their desirable mechanical, electrical and magnetic properties. Furthermore, crystallizing amorphous alloys provides an avenue for discerning sequential and competitive phases thus allowing access to otherwise inaccessible crystalline structures. Previous studies have shown the benefits of using AEM to determine crystal structures and compositions of partially crystallized alloys. The present paper will discuss the AEM characterization of crystallized Cu-Ti and Ni-Ti amorphous films.Cu60Ti40: The amorphous alloy Cu60Ti40, when continuously heated, forms a simple intermediate, macrocrystalline phase which then transforms to the ordered, equilibrium Cu3Ti2 phase. However, contrary to what one would expect from kinetic considerations, isothermal annealing below the isochronal crystallization temperature results in direct nucleation and growth of Cu3Ti2 from the amorphous matrix.


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