The effect of contact lens solutions on membrane permeability of Staphylococcus aureus aggregates

2013 ◽  
Vol 36 ◽  
pp. e40-e41
Author(s):  
David McCanna ◽  
Lyndon Jones
Keyword(s):  
Staphylococcus Aureus ◽  
Membrane Permeability ◽  
Contact Lens ◽  
Contact Lens Solutions

scholarly journals Component Analysis of Multipurpose Contact Lens Solutions To Enhance Activity against Pseudomonas aeruginosa and Staphylococcus aureus

2016 ◽  
Vol 60 (7) ◽  
pp. 4259-4263 ◽  
Author(s):  
Leo Lin ◽  
Janie Kim ◽  
Hope Chen ◽  
Regis Kowalski ◽  
Victor Nizet
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Contact Lens ◽  
Dilution Method ◽  
Microbial Keratitis ◽  
Content Type ◽  
Contact Lens Solutions ◽  
Contact Lens Solution ◽  
Broth Dilution ◽  
Solution Formulation

ABSTRACTMore than 125 million people wear contact lenses worldwide, and contact lens use is the single greatest risk factor for developing microbial keratitis. We tested the antibacterial activity of multipurpose contact lens solutions and their individual component preservatives against the two most common pathogens causing bacterial keratitis,Pseudomonas aeruginosaandStaphylococcus aureus. Thein vitroantibacterial activity of five multipurpose contact lens solutions (Opti-Free GP, Boston Simplus, Boston Advance, Menicare GP, and Lobob) was assayed by the standard broth dilution method. Synergy between the preservative components found in the top performing solutions was assayed using checkerboard and time-kill assays. The ISO 14729 criteria and the standard broth dilution method were used to define an optimized contact lens solution formulation against a clinical panel of drug-susceptible and drug-resistantP. aeruginosaandS. aureusstrains. Preservatives with the biguanide function group, chlorhexidine and polyaminopropylbiguanide (PAPB), had the best antistaphylococcal activity, while EDTA was the best antipseudomonal preservative. The combination of chlorhexidine and EDTA had excellent synergy againstP. aeruginosa. A solution formulation containing chlorhexidine (30 ppm), PAPB (5 ppm), and EDTA (5,000 ppm) had three to seven times more antipseudomonal activity than anything available to consumers today. A multipurpose contact lens solution containing a combination of chlorhexidine, PAPB, and EDTA could help to reduce the incidence of microbial keratitis for contact lens users worldwide.

scholarly journals A comparison of the ability of three common contact lens solutions with different constituents to inhibit growth of Staphylococcus aureus

2015 ◽  
Vol 74 (1) ◽  
Author(s):  
Marsha Oberholzer ◽  
Jacques Raubenheimer ◽  
Marga Lyell ◽  
Sade Pieterse ◽  
Aveli Keyser ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Contact Lens ◽  
Contact Lenses ◽  
Iso Standard ◽  
Iso Standards ◽  
Soft Contact Lenses ◽  
Log Reduction ◽  
Contact Lens Solutions ◽  
Mucosal Surfaces ◽  
Solution Saline

Background: Staphylococcus aureus is a common commensal on skin and mucosal surfaces; its contact with the eye may cause a variety of ocular inflammations and infections such as blepharitis, conjunctivitis and keratitis, amongst others. Soft contact lenses provide perfect conditions for the breeding of certain pathogens, and disinfecting solutions for contact lenses are therefore of utmost importance. These solutions should be effective in inhibiting the growth of a variety of pathogens to protect the user from ocular infections.Aim: To highlight the need for clinicians to be aware of the effects of various recommended disinfecting contact lens solutions.Method: Three popular disinfecting contact lens solutions readily available in South Africa were chosen. These and a control solution (saline) were prepared and inoculated with S. aureus to evaluate the antimicrobial efficacy of each solution. The primary stand-alone test was used to evaluate the solutions according to the ISO standard specifically for this purpose.Results: The test results indicated that two of the solutions met the ISO standards; the third failed. Of the two that passed the test, only one showed the required 3-log reduction after 30 minutes, as per the ISO standard, although this solution is marketed as a ’10 minute system’.Conclusion: It is important for clinicians to be aware of the complications that may be caused by contaminated solutions, and patients should be warned about the effects thereof. To ensure healthy eyes for our patients, sufficient knowledge regarding the efficacy of recommended multipurpose solutions is necessary. Solutions that meet ISO standards promote good ocular health and ensure sufficient cleaning and disinfecting of contact lenses.

pH OF MULTIPURPOSE CONTACT LENS SOLUTIONS OVER TIME

1998 ◽  
Vol 21 (1) ◽  
pp. 7-10
Author(s):  
Antonio López-Alemany ◽  
Robert Montés i Micó
Keyword(s):  
Contact Lens ◽  
Contact Lens Solutions ◽  
Over Time

scholarly journals Multiparameter Flow Cytometric Analysis of Antibiotic Effects on Membrane Potential, Membrane Permeability, and Bacterial Counts of Staphylococcus aureus andMicrococcus luteus

2000 ◽  
Vol 44 (4) ◽  
pp. 827-834 ◽  
Author(s):  
David J. Novo ◽  
Nancy G. Perlmutter ◽  
Richard H. Hunt ◽  
Howard M. Shapiro
Keyword(s):  
Staphylococcus Aureus ◽  
Flow Cytometry ◽  
Membrane Potential ◽  
Membrane Permeability ◽  
Micrococcus Luteus ◽  
Flow Cytometric Analysis ◽  
Bacterial Counts ◽  
Flow Cytometric ◽  
Permeability Parameter ◽  
Particle Counts

ABSTRACT Although flow cytometry has been used to study antibiotic effects on bacterial membrane potential (MP) and membrane permeability, flow cytometric results are not always well correlated to changes in bacterial counts. Using new, precise techniques, we simultaneously measured MP, membrane permeability, and particle counts of antibiotic-treated and untreated Staphylococcus aureus andMicrococcus luteus cells. MP was calculated from the ratio of red and green fluorescence of diethyloxacarbocyanine [DiOC2(3)]. A normalized permeability parameter was calculated from the ratio of far red fluorescence of the nucleic acid dye TO-PRO-3 and green DiOC2(3) fluorescence. Bacterial counts were calculated by the addition of polystyrene beads to the sample at a known concentration. Amoxicillin increased permeability within 45 min. At concentrations of <1 μg/ml, some organisms showed increased permeability but normal MP; this population disappeared after 4 h, while bacterial counts increased. At amoxicillin concentrations above 1 μg/ml, MP decreased irreversibly and the particle counts did not increase. Tetracycline and erythromycin caused smaller, dose- and time-dependent decreases in MP. Tetracycline concentrations of <1 μg/ml did not change permeability, while a tetracycline concentration of 4 μg/ml permeabilized 50% of the bacteria; 4 μg of erythromycin per ml permeabilized 20% of the bacteria. Streptomycin decreased MP substantially, with no effect on permeability; chloramphenicol did not change either permeability or MP. Erythromycin pretreatment of bacteria prevented streptomycin and amoxicillin effects. Flow cytometry provides a sensitive means of monitoring the dynamic cellular events that occur in bacteria exposed to antibacterial agents; however, it is probably simplistic to expect that changes in a single cellular parameter will suffice to determine the sensitivities of all species to all drugs.

Contact Lens Solutions

2010 ◽  
pp. 103-116
Author(s):  
Douglas J. Rhee ◽  
Kathryn A. Colby ◽  
Lucia Sobrin ◽  
Christopher J. Rapuano
Keyword(s):  
Contact Lens ◽  
Contact Lens Solutions

scholarly journals Determination of Amoebicidal Activities of Multipurpose Contact Lens Solutions by Using a Most Probable Number Enumeration Technique

2003 ◽  
Vol 41 (7) ◽  
pp. 2992-3000 ◽  
Author(s):  
T. K. Beattie ◽  
D. V. Seal ◽  
A. Tomlinson ◽  
A. K. McFadyen ◽  
A. M. Grimason
Keyword(s):  
Contact Lens ◽  
Most Probable Number ◽  
Probable Number ◽  
Contact Lens Solutions

scholarly journals Tannic acid-modified silver nanoparticles enhance anti-Acanthamoeba activity without increasing cytotoxicity of three multipurpose contact lens solutions

2020 ◽  
Author(s):  
Edyta Beata Hendiger ◽  
Marcin Padzik ◽  
Agnieszka Żochowska ◽  
Wanda Baltaza ◽  
Gabriela Olędzka ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Tannic Acid ◽  
Contact Lens ◽  
Contact Lenses ◽  
Colorimetric Assay ◽  
Statistical Significance ◽  
Plant Metabolites ◽  
Contact Lens Solutions ◽  
Preventive Actions

Abstract Background: Free living amoebae of Acanthamoeba genus are cosmopolitan, widely distributed protozoans causing severe, vision-threatening corneal infection known as Acanthamoeba keratitis (AK). Majority of the increasing number of AK cases are associated with contact lenses use. Due to lack of effective therapies against AK, proper eye hygiene and effective contact lenses disinfection are crucial in prevention of this infection. Currently available multipurpose contact lens disinfection systems are not fully effective against Acanthamoeba trophozoites and cysts. There is an urgent need to increase the disinfecting activity of these systems to prevent Acanthamoeba keratitis infections. Synthesized nanoparticles have been recently studied and proposed as a new generation of anti-microbial agents. It is also known that plant metabolites, including tannins, present anti-parasitic activity. The aim of this study was to evaluate the anti-amoebic activity and cytotoxicity of the tannic acid-modified silver nanoparticles (AgTANPs) conjugated with the selected multipurpose contact lens solutions.Methods: The anti-amoebic activity of pure contact lens care solutions and nanoparticles conjugated with contact lens care solutions were examined in vitro by colorimetric assay, based on the oxido-reduction of AlamarBlue. The cytotoxicity assays were performed using a fibroblast HS-5 (ATCC CRL-11882) cell line. The results were statistically analyzed by ANOVA and Student-Newman-Keuls tests using the p<0.05 level of a statistical significance.Results: The obtained results showed that nanoparticles enhanced anti-Acanthamoeba activity of the tested contact lens solutions without increasing their cytotoxicity profile. The activity is enhanced within minimal disinfection time recommended by the manufacturer.Conclusions: The conjugation of the selected contact lens solutions with AgTANPs might be a novel and promising approach as a part of preventive actions of Acanthamoeba keratitis infections among contact lens users.

Sign in / Sign up

Export Citation Format

Share Document