Effects of caffeic acid phenethyl ester on lipid peroxidation and antioxidant enzymes in diabetic rat heart

Oxidative stress is considered to be a major cause of cellular injuries in a variety of chronic health problems, such as carcinogenesis and neurodegenerative disorders. Caffeic acid phenethyl ester (CAPE), derived from the propolis of honeybee hives, possesses a variety of biological and pharmacological properties including antioxidant and anticancer activity. In the present study, we focused on the diverse antioxidative functionalities of CAPE and its related polyphenolic acid esters on cellular macromolecules in vitro. The effects on human erythrocyte membrane ghost lipid peroxidation, plasmid pBR322 DNA, and protein damage initiated by the water-soluble initiator 2,2′-azobis(2-amidinopropane) hydrochloride (AAPH) and hydrogen peroxide (H2O2) were monitored by formation of hydroperoxides and by DNA nicking assay, single-cell alkaline electrophoresis, and SDS-polyacrylamide gel electrophoresis. Our results showed that CAPE and its related polyphenolic acid esters elicited remarkable inhibitory effects on erythrocyte membrane lipid peroxidation, cellular DNA strand breakage, and protein fragmentation. The results suggest that CAPE is a potent exogenous cytoprotective and antigenotoxic agent against cell oxidative damage that could be used as a template for designing novel drugs to combat diseases induced by oxidative stress components, such as various types of cancer.

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Ischemia-reperfusion leads to depletion of glutathione content and augmentation of malondialdehyde production in the rat heart from overproduction of oxidants: Can caffeic acid phenethyl ester (CAPE) protect the heart?

Molecular and Cellular Biochemistry ◽

10.1007/s11010-005-0551-8 ◽

2005 ◽

Vol 273 (1-2) ◽

pp. 169-175 ◽

Cited By ~ 42

Author(s):

Mehmet Kaya Ozer ◽

Hakan Parlakpinar ◽

Yilmaz Cigremis ◽

Muharrem Ucar ◽

Nigar Vardi ◽

...

Keyword(s):

Caffeic Acid ◽

Rat Heart ◽

Ischemia Reperfusion ◽

Caffeic Acid Phenethyl Ester

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The effects of diazinon on lipid peroxidation and antioxidant enzymes in rat heart and ameliorating role of vitamin E and vitamin C

Cell Biology and Toxicology ◽

10.1007/s10565-006-0138-5 ◽

2006 ◽

Vol 22 (6) ◽

pp. 455-461 ◽

Cited By ~ 45

Author(s):

O. Akturk ◽

H. Demirin ◽

R. Sutcu ◽

N. Yilmaz ◽

H. Koylu ◽

...

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Enzymes ◽

Vitamin E ◽

Vitamin C ◽

Rat Heart

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Antidiabetic and Neuroprotective Effects of Trigonella Foenum-graecum Seed Powder in Diabetic Rat Brain

Prague Medical Report ◽

10.14712/23362936.2015.35 ◽

2012 ◽

Vol 113 (1) ◽

pp. 33-43 ◽

Cited By ~ 16

Author(s):

P. Kumar ◽

R. K. Kale ◽

P. McLean ◽

Najma Zaheer Baquer

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Enzymes ◽

Rat Brain ◽

Membrane Fluidity ◽

Diabetic Rats ◽

Diabetic Rat ◽

Neuroprotective Effects ◽

Trigonella Foenum Graecum ◽

Chronic Hyperglycemia ◽

Diabetic Brain

Trigonella foenum-graecum seed powder (TSP) has been reported to have hypoglycemic and hyperinsulinemic action. The objective of the study was to examine the antidiabetic and neuroprotective role of TSP in hyperglycemiainduced alterations in blood glucose, insulin levels and activities of membrane linked enzymes (Na+K+ATPase, Ca2+ATPase), antioxidant enzymes (superoxide dismutase, glutathione S-transferase), calcium (Ca2+) levels, lipid peroxidation, membrane fluidity and neurolipofuscin accumulation in the diabetic rat brain. Female Wistar rats weighing between 180 and 220 g were made diabetic by a single injection of alloxan monohydrate (15 mg/100 g body weight), diabetic rats were given 2 IU insulin, per day with 5% TSP in the diet for three weeks. A significant increase in lipid peroxidation was observed in diabetic brain. The increased lipid peroxidation following chronic hyperglycemia was accompanied with a significant increase in the neurolipofuscin deposition and Ca2+ levels with decreased activities of membrane linked ATPases and antioxidant enzymes in diabetic brain. A decrease in synaptosomal membrane fluidity may influence the activity of membrane linked enzymes in diabetes. The present study showed that TSP treatment can reverse the hyperglycemia induced changes to normal levels in diabetic rat brain. TSP administration amended effect of hyperglycemia on alterations in lipid peroxidation, restoring membrane fluidity, activities of membrane bound and antioxidant enzymes, thereby ameliorating the diabetic complications.

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Protective Potential of Azadirachta indica Leaf Extract in Diabetic Rat Liver

International Journal of Pharmacognosy and Phytochemical Research ◽

10.25258/phyto.v9i2.8059 ◽

2017 ◽

Vol 9 (2) ◽

Author(s):

Gupta N.K. ◽

Srivastva N. ◽

Bubber P. ◽

Garg S. ◽

Mohammad O.

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Enzymes ◽

Azadirachta Indica ◽

Liver Tissue ◽

Major Complication ◽

Tissue Level ◽

Diabetic Rats ◽

Diabetic Rat ◽

Leaf Extracts ◽

Glucose Levels

Diabetes mellitus is a debilitating metabolic disorder and emerged as a major complication nowadays. The role of plants and their derivatives is indispensable in the treatment of various diseases. Azadirachta indica is a medicinal plant and holds a potential to attenuate the pathological changes associated with diabetes. In the present study streptozotocin (STZ) induced diabetic rats received the Azadirachta indica leaf extracts (ALE) treatment for a period of seven consecutive days (600 mg/Kg body weight) and then evaluated for changes in the liver tissue. The diabetic rats exhibited significant (p≤0.001) increase in the lipid peroxidation levels, decrease in GSH levels and modulation in the activities of various antioxidant enzymes including catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase and glutathione-S-transferase. However, following ALE treatment to diabetic rats reported decrease in the blood glucose levels, lipid peroxidation levels, improved GSH levels and restored the activities of various antioxidant enzymes towards control levels. Besides this, light microscopic and ultramicroscopic examination of liver tissue also demonstrated less fragmentation, inflammation and decreased tissue level damage after ALE treatment, and corroborated the results of biochemical parameters. Thus, the results of present study revealed the hepatoprotective potential of Azadirachta indica in diabetes

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