lncRNA RP5-916L7.2 correlates with advanced tumor stage, and promotes cells proliferation while inhibits cells apoptosis through targeting miR-328 and miR-939 in tongue squamous cell carcinoma

Abstract Objective: To analyze the prognostic value of frequency of heterotypic neutrophil-in-tumor structure(FNiT) in patients with tongue squamous cell carcinoma(TSCC).Methods: In vitro, we cocultured TSCC cell line-CAL33 with neutrophils to form heterotypic neutrophil-in-tumor structures, which were then subject to fluorescence staining. Clinically, 197 patients were enrolled. Information including age, sex, FNiT, ECOG PS(Performance Status), FH (family history) of cancer, complications, and pathological characteristics such as tumor stage, node stage, metastasis, disease stage, lymphovascular invasion, perineural invasion, tumor grade, and follow-up results was extracted and analyzed.Results: Fluorescent staining results of typical heterotypic neutrophil-in-tumor structure showed that well-differentiated CAL-33-2 had stronger ability to internalize more neutrophils than poorly-differentiated CAL-33-1 did, the latter often internalizing only one neutrophil. The mean FNiT was 4.8‰,with a range from 2.1‰ to 8.9‰. The FNiT was significantly associated with tumor stage, disease stage and tumor grade. A total of 119 patients died of the disease, and the 5-year disease-specific survival(DSS) rate was 36%. The median survival time was 52.6 months. In patients with an FNiT<4.8‰, the 5-year DSS rate was 40%; in patients with an FNiT>=4.8‰, the 5-year DSS was 28%, and the difference was significant(P=0.001). Cox model analysis showed that FNiT along with disease stage, lymphovascular invasion and tumor grade was an independent prognostic factor for DSS.Conclusion: The FNiT as a novel predictor is positively correlated with adverse prognosis of patients with TSCC.

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The regulation of acetylation and stability of HMGA2 via the HBXIP-activated Akt–PCAF pathway in promotion of esophageal squamous cell carcinoma growth

Nucleic Acids Research ◽

10.1093/nar/gkaa232 ◽

2020 ◽

Vol 48 (9) ◽

pp. 4858-4876 ◽

Cited By ~ 2

Author(s):

Yue Wu ◽

Xue Wang ◽

Feifei Xu ◽

Lu Zhang ◽

Tianjiao Wang ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Esophageal Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Binding Capacity ◽

Disease Free Survival ◽

Protein Accumulation ◽

Advanced Tumor Stage ◽

Advanced Tumor ◽

Hmga2 Protein

Abstract High-mobility group AT-hook 2 (HMGA2) is an architectural transcription factor that plays essential roles in embryonic development and cancer progression. However, the mechanism of HMGA2 regulation remains largely uncharacterized. Here, we demonstrate that HMGA2 can be modulated by hepatitis B X-interacting protein (HBXIP), an oncogenic transcriptional coactivator, in esophageal squamous cell carcinoma (ESCC). HMGA2 expression was positively associated with HBXIP expression in clinical ESCC tissues, and their high levels were associated with advanced tumor stage and reduced overall and disease-free survival. We found that oncogenic HBXIP could posttranslationally upregulate HMGA2 protein level in ESCC cells. HBXIP induced HMGA2 acetylation at the lysine 26 (K26), resulting in HMGA2 protein accumulation. In this process, HBXIP increased the acetyltransferase p300/CBP-associated factor (PCAF) phosphorylation and activation via the Akt pathway, then PCAF directly interacted with HMGA2, leading to HMGA2 acetylation in the cells. HMGA2 K26 acetylation enhanced its DNA binding capacity and blocked its ubiquitination and then inhibited proteasome-dependent degradation. Functionally, HBXIP-stabilized HMGA2 could promote ESCC cell growth in vitro and in vivo. Strikingly, aspirin suppressed ESCC growth by inhibiting HBXIP and HMGA2. Collectively, our findings disclose a new mechanism for the posttranslational regulation of HMGA2 mediated by HBXIP in ESCC.

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SDCBP Modulates Stemness and Chemoresistance in Head and Neck Squamous Cell Carcinoma through Src Activation

Cancers ◽

10.3390/cancers13194952 ◽

2021 ◽

Vol 13 (19) ◽

pp. 4952

Author(s):

Cristina Mir ◽

Yoelsis Garcia-Mayea ◽

Laia Garcia ◽

Pol Herrero ◽

Nuria Canela ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Head And Neck ◽

Squamous Cell ◽

Survival Rates ◽

Neck Squamous Cell Carcinoma ◽

Advanced Tumor Stage ◽

Ki 67 ◽

Advanced Tumor ◽

Src Activation

To characterize the mechanisms that govern chemoresistance, we performed a comparative proteomic study analyzing head and neck squamous cell carcinoma (HNSCC) cells: CCL-138 (parental), CCL-138-R (cisplatin-resistant), and cancer stem cells (CSCs). Syntenin-1 (SDCBP) was upregulated in CCL-138-R cells and CSCs over parental cells. SDCBP depletion sensitized biopsy-derived and established HNSCC cell lines to cisplatin (CDDP) and reduced CSC markers, Src activation being the main SDCBP downstream target. In mice, SDCBP-depleted cells formed tumors with decreased mitosis, Ki-67 positivity, and metastasis over controls. Moreover, the fusocellular pattern of CCL-138-R cell-derived tumors reverted to a more epithelial morphology upon SDCBP silencing. Importantly, SDCBP expression was associated with Src activation, poor differentiated tumor grade, advanced tumor stage, and shorter survival rates in a series of 382 HNSCC patients. Our results reveal that SDCBP might be a promising therapeutic target for effectively eliminating CSCs and CDDP resistance.

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Immunohistochemical Expression of TGF-β3 in Oral Squamous Cell Carcinoma

International Journal of Pharmaceutical Quality Assurance ◽

10.25258/ijpqa.10.1.28 ◽

2019 ◽

Vol 10 (01) ◽

Author(s):

Asmaa Ali Hussein

Keyword(s):

Squamous Cell Carcinoma ◽

Oral Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Transforming Growth Factor ◽

Tumor Grade ◽

Tumor Stage ◽

High Rate ◽

Female Ratio ◽

Positive Expression

Squamous cell carcinoma characterized by poor prognosis due to aggressive tumor growth and dissemination high rate of tumor cell . age ranged of patient case included in the study 40-62 years and mean age 55±99. The sex distribution male/female ratio 1:1. Male case 15 and female 15 of the present study The results of clinical forums showed in the current study was endophytic 10(33.3%) in the same time Exophytic were presented in 20 cases (76.7%). Regarding distribution of the tumors site, the preponderance of them 19 cases 73.3% were located alveolar mucosa, followed by in the tongue 11 cases(36.7%) Tumor stage was analyzed and recorded in Oral squamous cell carcinoma included cases, the preponderance of them were Stage II 11 cases 36.7% followed by stage III 10 cases 33.3% , 9 cases 30.0% were stage I. While Concerning tumor grade, majority of them 15 cases 50% had grade II moderately differentiated SCC, while 11 cases 36.7% had grade III poorly differentiated SCC and 4 cases 13.3% had grade I well differentiated SCC Positive TGF-β3 immunostaining was detected as cell with staining brown color, all tissues sections included show Positive expression based on IHC teqnique. Positive Transforming Growth Factor TGF-β3 Immuno staining was found in all case results and display that 4 samples with percentage 13.3% expressed strong positive 87.67 ± 1.45 expression , 11cases 36.7% showed 51.33 ±0.88 positive expression moderate at the same time 15 samples 50.0% showed positive weak expression.

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