Investigation of the elastic and adhesion properties of adsorbed hydrophobically modified inulin films on latex particles using Atomic Force Microscopy (AFM)

Author(s):  
M. Obiols-Rabasa ◽  
G. Oncins ◽  
F. Sanz ◽  
Th.F. Tadros ◽  
C. Solans ◽  
...  
Author(s):  
O. Shaffer ◽  
J. Qian ◽  
V. Dimonie ◽  
R. Pearson ◽  
M. El-Aasser

Atomic force microscopy (AFM) is a powerful scanning probe technique, which is capable of imaging polymer surfaces. This technique is complementary to the scanning electron microscope (SEM) but because of the AFM's sensitivity in the z direction surfaces that are too smooth to image by SEM can easily be imaged by AFM. This study utilizes transmission electron microscopy(TEM) to image the morphology of the latex rubber particles; SEM and AFM are used to study the degree of dispensability of the latex particles in the epoxy, and the fatigue-fracture surface of the rubber modified epoxy.Core-shell latex particles were prepared with a core of poly(butadiene-styreiie) |P(B-S)| and a shell Poly(methyl methacrylate)(PMMA). In order to study the interaction between the core/shell particles and the epoxy matrix, the shell is systematically varied in terms of chemical bonding, physical interaction and the extent of these interactions by incorporating acrylonitrile(AN). glycidyl-methacrylate(GMA). and crosslinking agent divinylbenzene(DVB) of varying concentrations in the shell.


Microbiology ◽  
2014 ◽  
Vol 160 (7) ◽  
pp. 1466-1473 ◽  
Author(s):  
Shivani Sharma ◽  
Stacey Lavender ◽  
JungReem Woo ◽  
Lihong Guo ◽  
Wenyuan Shi ◽  
...  

A major aetiological factor of dental caries is the pathology of the dental plaque biofilms. The amino acid l-arginine (Arg) is found naturally in saliva as a free molecule or as a part of salivary peptides and proteins. Plaque bacteria metabolize Arg to produce alkali and neutralize glycolytic acids, promoting a less cariogenous oral microbiome. Here, we explored an alternative and complementary mechanism of action of Arg using atomic force microscopy. The nanomechanical properties of Streptococcus mutans biofilm extracellular matrix were characterized under physiological buffer conditions. We report the effect of Arg on the adhesive behaviour and structural properties of extracellular polysaccharides in S. mutans biofilms. High-resolution imaging of biofilm surfaces can reveal additional structural information on bacterial cells embedded within the surrounding extracellular matrix. A dense extracellular matrix was observed in biofilms without Arg compared to those grown in the presence of Arg. S. mutans biofilms grown in the presence of Arg could influence the production and/or composition of extracellular membrane glucans and thereby affect their adhesion properties. Our results suggest that the presence of Arg in the oral cavity could influence the adhesion properties of S. mutans to the tooth surface.


2002 ◽  
Vol 18 (7) ◽  
pp. 763-766 ◽  
Author(s):  
I. C. Gebeshuber ◽  
J. B. Thompson ◽  
Y. Del Amo ◽  
H. Stachelberger ◽  
J. H. Kindt

2018 ◽  
Vol 32 (3) ◽  
pp. e2767 ◽  
Author(s):  
Lionel Chièze ◽  
Anthony Le Cigne ◽  
Marie Meunier ◽  
Alexandre Berquand ◽  
Stéphane Dedieu ◽  
...  

2017 ◽  
Vol 139 (9) ◽  
Author(s):  
Vishwanath Managuli ◽  
Sitikantha Roy

A new asymptotically correct contact model has been developed for conical tip based atomic force microscopy (AFM) nanoindentation. This new model provides both elastic and nonspecific adhesion properties of cells and soft gels by taking sample thickness at the point of indentation and its depth of indentation into consideration. The bottom substrate effect (BSE) is the most common source of error in the study of “AFM force maps” of the cellular sample. The present model incorporates an asymptotically correct correction term as a function of depth of indentation to eliminate the substrate effect in the analysis. Later, the model is extended to analyze the unloading portion of the indentation curve to extract the stiffness and adhesive properties simultaneously. A comparative study of the estimated material properties using other established contact models shows that the provided corrections effectively curb the errors coming from infinite thickness assumption. Nonspecific adhesive nature of a cell is represented in terms of adhesion parameter (γa) based on the “work of adhesion,” this is an alternative to the peak value of tip–sample attractive (negative) force commonly used as representative adhesion measurement. The simple analytical expression of the model can help in estimating more realistic and accurate biomechanical properties of cells from atomic force microscopy based indentation technique.


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