Probing friction and adhesion properties of poly(vinyl methylether) homopolymer and blend films under nano-confinement using atomic-force microscopy

A major aetiological factor of dental caries is the pathology of the dental plaque biofilms. The amino acid l-arginine (Arg) is found naturally in saliva as a free molecule or as a part of salivary peptides and proteins. Plaque bacteria metabolize Arg to produce alkali and neutralize glycolytic acids, promoting a less cariogenous oral microbiome. Here, we explored an alternative and complementary mechanism of action of Arg using atomic force microscopy. The nanomechanical properties of Streptococcus mutans biofilm extracellular matrix were characterized under physiological buffer conditions. We report the effect of Arg on the adhesive behaviour and structural properties of extracellular polysaccharides in S. mutans biofilms. High-resolution imaging of biofilm surfaces can reveal additional structural information on bacterial cells embedded within the surrounding extracellular matrix. A dense extracellular matrix was observed in biofilms without Arg compared to those grown in the presence of Arg. S. mutans biofilms grown in the presence of Arg could influence the production and/or composition of extracellular membrane glucans and thereby affect their adhesion properties. Our results suggest that the presence of Arg in the oral cavity could influence the adhesion properties of S. mutans to the tooth surface.

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In vivonanoscale atomic force microscopy investigation of diatom adhesion properties

Materials Science and Technology ◽

10.1179/026708302225003857 ◽

2002 ◽

Vol 18 (7) ◽

pp. 763-766 ◽

Cited By ~ 27

Author(s):

I. C. Gebeshuber ◽

J. B. Thompson ◽

Y. Del Amo ◽

H. Stachelberger ◽

J. H. Kindt

Keyword(s):

Atomic Force Microscopy ◽

Adhesion Properties ◽

Force Microscopy ◽

Atomic Force Microscopy Investigation ◽

Atomic Force ◽

Microscopy Investigation

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Numerical and experimental evaluation of adhesion properties of asphalt-aggregate interfaces using molecular dynamics simulation and atomic force microscopy

Road Materials and Pavement Design ◽

10.1080/14680629.2021.1910547 ◽

2021 ◽

pp. 1-21

Author(s):

Bingyan Cui ◽

Xingyu Gu ◽

Hao Wang ◽

Dongliang Hu

Keyword(s):

Molecular Dynamics ◽

Atomic Force Microscopy ◽

Molecular Dynamics Simulation ◽

Experimental Evaluation ◽

Dynamics Simulation ◽

Adhesion Properties ◽

Force Microscopy ◽

Atomic Force

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Quantitative characterization of single-cell adhesion properties by atomic force microscopy using protein-functionalized microbeads

Journal of Molecular Recognition ◽

10.1002/jmr.2767 ◽

2018 ◽

Vol 32 (3) ◽

pp. e2767 ◽

Cited By ~ 3

Author(s):

Lionel Chièze ◽

Anthony Le Cigne ◽

Marie Meunier ◽

Alexandre Berquand ◽

Stéphane Dedieu ◽

...

Keyword(s):

Atomic Force Microscopy ◽

Cell Adhesion ◽

Single Cell ◽

Quantitative Characterization ◽

Adhesion Properties ◽

Force Microscopy ◽

Atomic Force

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Simultaneous Analysis of Elastic and Nonspecific Adhesive Properties of Thin Sample and Biological Cell Considering Bottom Substrate Effect

Journal of Biomechanical Engineering ◽

10.1115/1.4037289 ◽

2017 ◽

Vol 139 (9) ◽

Cited By ~ 5

Author(s):

Vishwanath Managuli ◽

Sitikantha Roy

Keyword(s):

Atomic Force Microscopy ◽

Correction Term ◽

Work Of Adhesion ◽

Common Source ◽

Substrate Effect ◽

Adhesive Properties ◽

Adhesion Properties ◽

Force Microscopy ◽

Bottom Substrate ◽

Atomic Force

A new asymptotically correct contact model has been developed for conical tip based atomic force microscopy (AFM) nanoindentation. This new model provides both elastic and nonspecific adhesion properties of cells and soft gels by taking sample thickness at the point of indentation and its depth of indentation into consideration. The bottom substrate effect (BSE) is the most common source of error in the study of “AFM force maps” of the cellular sample. The present model incorporates an asymptotically correct correction term as a function of depth of indentation to eliminate the substrate effect in the analysis. Later, the model is extended to analyze the unloading portion of the indentation curve to extract the stiffness and adhesive properties simultaneously. A comparative study of the estimated material properties using other established contact models shows that the provided corrections effectively curb the errors coming from infinite thickness assumption. Nonspecific adhesive nature of a cell is represented in terms of adhesion parameter (γa) based on the “work of adhesion,” this is an alternative to the peak value of tip–sample attractive (negative) force commonly used as representative adhesion measurement. The simple analytical expression of the model can help in estimating more realistic and accurate biomechanical properties of cells from atomic force microscopy based indentation technique.

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Effect of Alginate Lyase on Biofilm-GrownHelicobacter pyloriProbed by Atomic Force Microscopy

International Journal of Polymer Science ◽

10.1155/2015/989516 ◽

2015 ◽

Vol 2015 ◽

pp. 1-9 ◽

Cited By ~ 12

Author(s):

Alessandro Maiorana ◽

Francesca Bugli ◽

Massimiliano Papi ◽

Riccardo Torelli ◽

Gabriele Ciasca ◽

...

Keyword(s):

Atomic Force Microscopy ◽

Extracellular Polymeric Substances ◽

Alginate Lyase ◽

Adhesion Properties ◽

Force Microscopy ◽

Coccoid Form ◽

Atomic Force ◽

Biofilm Architecture ◽

H Pylori ◽

New Strategies

Helicobacter pylori(H. pylori) is a microorganism with a pronounced capability of adaptation under environmental stress solicitations. Its persistence and antimicrobial resistance to the drugs commonly used in the anti-H. pyloritherapy are associated with the development of a biofilm mainly composed of DNA, proteins, and polysaccharides. A fundamental step to increase the success of clinical treatments is the development of new strategies and molecules able to interfere with the biofilm architecture and thus able to enhance the effects of antibiotics. By using Atomic Force Microscopy and Scanning Electron Microscopy we analyzed the effects of the alginate lyase (AlgL), an enzyme able to degrade a wide class of polysaccharides, on theH. pylorishape, surface morphology, and biofilm adhesion properties. We demonstrated that AlgL generates a noticeable loss ofH. pyloricoccoid form in favor of the bacillary form and reduces theH. pyloriextracellular polymeric substances (EPS).

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