Anti-oxidant, anti-bacterial and anti-biofilm activity of biosynthesized silver nanoparticles using Gracilaria corticata against biofilm producing K. pneumoniae

Author(s):  
Govindan Nadar Rajivgandhi ◽  
Govindan Ramachandran ◽  
Muthuchamy Maruthupandy ◽  
Natesan Manoharan ◽  
Naiyf S. Alharbi ◽  
...  
2021 ◽  
Vol 17 ◽  
Author(s):  
Amita Sahu ◽  
Sudhanshu Shekhar Swain ◽  
Goutam Ghosh ◽  
Deepak Pradhan ◽  
Dipak Kumar Sahu ◽  
...  

Background: Literature evidences as well as traditional uses of genus Alphonsea reveal significant antimicrobial and anti-oxidant activity, which encourages to consider A. madraspatana to have potent antimicrobials, there by offering potential adjuncts to synthesize improved antimicrobial Silver nanoparticles (AgNPs). The objective of the present exposition is to optimize reaction parameters to synthesize antimicrobial Biogenic Silver nanoparticles (BAgNPs) from extract of A. madraspatana leaves (AML) and to evaluate the effect against bacteria. Methods: BAgNPs was synthesized by optimized reaction. The Synthesized nanoparticles were characterized by UV, IR, ICP-MS and XRD analysis. The antibacterial potency of optimized BAgNPs was evaluated against E. coli by comparing with positive controls. Results: Results of optimization process indicate nanoscale BAgNPs were produced at operating temp. of 45°C for 120 min at pH 8 with 1:5 volume ratio of AgNO3 and extract. Optimized BAgNPs exhibits relatively higher antimicrobial activity (31±1mm) compared to Ciprofloxacin (27±1mm) and marketed nano silver (28± 2 mm). The developed BAgNPs shows comparable biofilm inhibition (86.50%) as compared to marketed nano silver (88.10%) and Ciprofloxacin (83.10%). Conclusion: Experimental evidence suggests methanolic extract of AML under predefined conditions successfully generate nano-template of silver with better antibacterial response against E. coli.


2018 ◽  
Vol 8 (3) ◽  
pp. 407-415 ◽  
Author(s):  
Anas Ejaz Shaikh ◽  
Kshitij Vasant Satardekar ◽  
Rummana Rehman Khan ◽  
Nanda Amit Tarte ◽  
Siddhivinayak Satyasandha Barve

Author(s):  
Taanya Imtiaz ◽  
R. Priyadharshini ◽  
S. Rajeshkumar ◽  
Palati Sinduja

Background: Piper longum, a traditional plant used for regenerative medicine commonly known as the long pepper, is used to treat many pathological conditions. Piper longum is used as a source for obtaining the production of various nanoparticles and testing their efficacy. Piper longum has many beneficial activities, such as antifungal, anti-amoebic, anti-asthmatic, anti-cancer, anti-oxidant, anti-inflammatory, antidepressants activities. Silver nanoparticles are widely and commonly used for its antimicrobial activity against various microorganisms. Our study aimed to synthesize Piper longum based silver nanoparticles and to investigate its anti-oxidant property. Materials and Methods: Piper longum plant extract was prepared and silver nanoparticles extract was synthesized. DPPH assay was then performed to assess the free radical scavenging activity of the Piper longum silver nanoparticle extract. The further analysis was that the formation of brownish-red color observed was evident, resulting in thesynthesization of silver nanoparticles. Results: Brown discolouration represents formation of silver nanoparticles and the antioxidant activity was noted to be from 60% to 70% from 10 to 50μL. But when compared to standard vitamin C values the Piper longum silver nanoparticles showed less antioxidant activity. Conclusion: The Piper longum mediated silver nanoparticles showed remarkable and considerable antioxidant activity but when compared with the standard sample values the antioxidant activity was low.


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