Optimization and Characterization of Biogenic Silver Nanoparticles Synthesized by Leaves Extract of Alphonsea Madraspatana

2021 ◽  
Vol 17 ◽  
Author(s):  
Amita Sahu ◽  
Sudhanshu Shekhar Swain ◽  
Goutam Ghosh ◽  
Deepak Pradhan ◽  
Dipak Kumar Sahu ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Volume Ratio ◽  
Xrd Analysis ◽  
Biofilm Inhibition ◽  
Nano Silver ◽  
E Coli ◽  
Biogenic Silver Nanoparticles ◽  
Icp Ms ◽  
Anti Oxidant

Background: Literature evidences as well as traditional uses of genus Alphonsea reveal significant antimicrobial and anti-oxidant activity, which encourages to consider A. madraspatana to have potent antimicrobials, there by offering potential adjuncts to synthesize improved antimicrobial Silver nanoparticles (AgNPs). The objective of the present exposition is to optimize reaction parameters to synthesize antimicrobial Biogenic Silver nanoparticles (BAgNPs) from extract of A. madraspatana leaves (AML) and to evaluate the effect against bacteria. Methods: BAgNPs was synthesized by optimized reaction. The Synthesized nanoparticles were characterized by UV, IR, ICP-MS and XRD analysis. The antibacterial potency of optimized BAgNPs was evaluated against E. coli by comparing with positive controls. Results: Results of optimization process indicate nanoscale BAgNPs were produced at operating temp. of 45°C for 120 min at pH 8 with 1:5 volume ratio of AgNO3 and extract. Optimized BAgNPs exhibits relatively higher antimicrobial activity (31±1mm) compared to Ciprofloxacin (27±1mm) and marketed nano silver (28± 2 mm). The developed BAgNPs shows comparable biofilm inhibition (86.50%) as compared to marketed nano silver (88.10%) and Ciprofloxacin (83.10%). Conclusion: Experimental evidence suggests methanolic extract of AML under predefined conditions successfully generate nano-template of silver with better antibacterial response against E. coli.

scholarly journals Green ultrasonic synthesis, Characterization and Antibacterial activity of Silver and Gold Nanoparticles mediated by Ganoderma lucidum extract

2020 ◽  
Vol 4 (2) ◽  
Keyword(s):  
Gold Nanoparticles ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Metal Nanoparticles ◽  
Cytotoxic Activities ◽  
Gold And Silver Nanoparticles ◽  
Silver And Gold Nanoparticles ◽  
E Coli ◽  
Inhibition Zones

Metal nanoparticles possess an extensive scientific and technological significance due to their unique physiochemical properties and their potential applications in different fields like medicine. Silver and gold nanoparticles have shown to have antibacterial and cytotoxic activities. Conventional methods used in the synthesis of the metal nanoparticles involve use of toxic chemicals making them unsuitable for use in medical field. In our continued effort to explore for simple and eco-friendly methods to synthesize the metal nanoparticles, we here describe synthesis and characterization of gold and silver nanoparticles using Gonaderma lucidum, wild non-edible medicinal mushroom. G. lucidum mushroom contain bioactive compounds which can be involved in the reduction, capping and stabilization of the nanoparticles. Antibacterial activity analysis was done on E. coli and S. aureus. The synthesis was done on ultrasonic bath. Characterization of the metal nanoparticles was done by UV-VIS., High Resolution Transmission Electron Microscope (HRTEM) and FTIR. HRTEM analysis showed that both silver and gold nanoparticles were spherical in shape with an average size of 15.82±3.69 nm for silver and 24.73±5.124nm for gold nanoparticles (AuNPs). FTIR analysis showed OH and -C=C- stretching vibrations, an indication of presence of functional groups of biomolecules capping both gold and silver nanoparticles. AgNPs showed inhibition zones of 15.5±0.09mm and 13.3±0.14mm while AuNPs had inhibition zones of 14.510±0.35 and 13.3±0.50mm on E. coli and S. aureus respectively. The findings indicate the potential use of AgNPs and AuNPs in development of drugs in management of pathogenic bacteria.

scholarly journals Green synthesis and Characterization of Silver Nanoparticles Synthesized Using Piper longum and its Antioxidant Activity

2021 ◽  
pp. 342-352
Author(s):  
Taanya Imtiaz ◽  
R. Priyadharshini ◽  
S. Rajeshkumar ◽  
Palati Sinduja
Keyword(s):  
Antioxidant Activity ◽  
Silver Nanoparticles ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Piper Longum ◽  
Pathological Conditions ◽  
Red Color ◽  
Anti Cancer ◽  
Anti Oxidant

Background: Piper longum, a traditional plant used for regenerative medicine commonly known as the long pepper, is used to treat many pathological conditions. Piper longum is used as a source for obtaining the production of various nanoparticles and testing their efficacy. Piper longum has many beneficial activities, such as antifungal, anti-amoebic, anti-asthmatic, anti-cancer, anti-oxidant, anti-inflammatory, antidepressants activities. Silver nanoparticles are widely and commonly used for its antimicrobial activity against various microorganisms. Our study aimed to synthesize Piper longum based silver nanoparticles and to investigate its anti-oxidant property. Materials and Methods: Piper longum plant extract was prepared and silver nanoparticles extract was synthesized. DPPH assay was then performed to assess the free radical scavenging activity of the Piper longum silver nanoparticle extract. The further analysis was that the formation of brownish-red color observed was evident, resulting in thesynthesization of silver nanoparticles. Results: Brown discolouration represents formation of silver nanoparticles and the antioxidant activity was noted to be from 60% to 70% from 10 to 50μL. But when compared to standard vitamin C values the Piper longum silver nanoparticles showed less antioxidant activity. Conclusion: The Piper longum mediated silver nanoparticles showed remarkable and considerable antioxidant activity but when compared with the standard sample values the antioxidant activity was low.

Rapid size characterization of silver nanoparticles by single particle ICP-MS and isotope dilution

2014 ◽  
Vol 29 (7) ◽  
pp. 1265-1272 ◽  
Author(s):  
L. Telgmann ◽  
C. D. Metcalfe ◽  
H. Hintelmann
Keyword(s):  
Silver Nanoparticles ◽  
Isotope Dilution ◽  
Single Particle ◽  
Consumer Products ◽  
Icp Ms ◽  
Size Characterization ◽  
Intensive Investigation

The increasing application of silver nanoparticles (AgNP) in consumer products and their potential release into the environment call for intensive investigation of their toxicity, stability, and fate.

scholarly journals Multivariate Model Based on UV-Vis Spectroscopy and Regression in Partial Least Squares for Determination of Diameter and Polydispersity of Silver Nanoparticles in Colloidal Suspensions

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
J. F. B. Rodrigues ◽  
E. P. S. Junior ◽  
K. S. Oliveira ◽  
M. R. R. Wellen ◽  
S. S. Simões ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Multivariate Calibration ◽  
Volume Ratio ◽  
Colloidal Suspensions ◽  
Prediction Errors ◽  
Predictive Capacity ◽  
Vis Spectroscopy ◽  
Characterization Of Properties ◽  
The One

In the universe of nanomaterials, silver nanoparticles (AgNPs) have attracted the attention of researchers because of their optical, catalytic, antimicrobial, fungicidal, and bactericidal properties. Recently, studies have correlated the toxicity and efficacy of antimicrobial activity with surface-volume ratio, morphology, polydispersity, ligand types, particle size, and stability of AgNPs. Soon, the need for characterization of properties such as diameter and polydispersity is clear. The methodologies conventionally used for characterization of AgNPs, although accurate, are generally expensive and laborious and can degrade the sample. Thus, the development of methodologies based on UV-Vis spectroscopy and chemometric techniques appears as an alternative for the characterization of diameter and polydispersity of the nanoparticles. For the development of the methodology in question, 50 samples were synthesized, varying the type, volume, and concentration of the reagents in order to increase the diameter and polydispersity values. All samples were analyzed by DLS and UV-Vis spectroscopy. For the construction of multivariate calibration models, the calibration and validation sets were selected using the SPXY algorithm, and their predictive capacity was evaluated based on the method figures. The model that presented the best predictive capacity was the one built with the pretreated spectra with the 1st derivative with a 15-point window and 2nd-order polynomial, providing prediction errors of 5.31% and 4.43% for diameter and polydispersity, respectively.

scholarly journals Bacteria Exposed to Silver Nanoparticles Synthesized by Laser Ablation in Water: Modelling E. coli Growth and Inactivation

Materials ◽  
2020 ◽  
Vol 13 (3) ◽  
pp. 653 ◽  
Author(s):  
Lucija Krce ◽  
Matilda Šprung ◽  
Ana Maravić ◽  
Polona Umek ◽  
Krešimir Salamon ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Bacterial Growth ◽  
Growth Curves ◽  
Dynamic State ◽  
Lag Phase ◽  
The Novel ◽  
Minimal Inhibitory Concentrations ◽  
E Coli ◽  
Main Effect

This study is aimed to better understand the bactericidal mode of action of silver nanoparticles. Here we present the production and characterization of laser-synthesized silver nanoparticles along with growth curves of bacteria treated at sub-minimal and minimal inhibitory concentrations, obtained by optical density measurements. The main effect of the treatment is the increase of the bacterial apparent lag time, which is very well described by the novel growth model as well as the entire growth curves for different concentrations. The main assumption of the model is that the treated bacteria uptake the nanoparticles and inactivate, which results in the decrease of both the nanoparticles and the bacteria concentrations. The lag assumes infinitive value for the minimal inhibitory concentration treatment. This apparent lag phase is not postponed bacterial growth. It is a dynamic state in which the bacterial growth and death rates are close in value. Our results strongly suggest that the predominant mode of antibacterial action of silver nanoparticles is the penetration inside the membrane.

scholarly journals Green Synthesis and Characterization of Silver Nanoparticles using Crude Extract of Crotalaria burhia

2021 ◽  
Vol 8 (4) ◽  
Author(s):  
Khalil Ahmad ◽  
Raeesa Noor ◽  
Muhammad Younus ◽  
Akram Chohan ◽  
Ume Habiba ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Zeta Potential ◽  
Green Synthesis ◽  
Crude Extract ◽  
Bacterial Infections ◽  
Synthesis And Characterization ◽  
Phytochemical Analysis ◽  
E Coli

Background: Appearance of antibiotic resistance has raised the demand to find alternative therapies and modified drug delivery system of medicinal plants to treat bacterial infections. Objective: The aim of this study is the green synthesis and characterization of silver nanoparticles by using crude extract of Crotalaria burhia and to evaluate their antibacterial potential. Methods: The roots and stems of plant were used to prepare the crude extract. The phytochemical analysis of different compounds in extract was performed. 1mM AgNO3 and different concentrations of plant extract were used for the green synthesis of silver nanoparticles. The particles size and zeta potential were measured by zeta sizer while surface morphology of silver nanoparticles was observed with Scanning Electron Microscope (SEM). The antibacterial activity of silver nanoparticles was performed by 96 well microdilution plate method. Results: The particle size and zeta potential of optimized formulation was 92 nm and -24.8 mV. The SEM analysis showed that silver nanoparticles are irregular and spherical shape. The antibacterial activity showed that MIC value of silver nanoparticles was lower for E. coli than S. aureus. Conclusion: Silver nanoparticles possess potent bactericidal activity against E. coli and moderate activity against S. aureus. It had been concluded that these nanoparticles can be used against multi-drug resistant bacterial infections.

scholarly journals Truncation of poly-N-acetylglucosamine (PNAG) polymerization with N-acetylglucosamine analogues

2021 ◽  
Author(s):  
Zachary Morrison ◽  
Alexander Eddenden ◽  
Adithya S Subramanian ◽  
P. Lynne Howell ◽  
mark nitz
Keyword(s):  
Biofilm Formation ◽  
Chain Termination ◽  
Salvage Pathway ◽  
Biofilm Inhibition ◽  
E Coli ◽  
Substrate Analogues

Bacteria require polysaccharides for structure, survival, and virulence. Despite the central role these structures play in microbiology few tools are available to manipulate their production. In E. coli the glycosyltransferase complex PgaCD produces poly-N-acetylglucosamine (PNAG), an extracellular matrix polysaccharide required for biofilm formation. We report that C6-substituted (H, F, N3, SH, NH2) UDP-GlcNAc substrate analogues are inhibitors of PgaCD. In vitro the inhibitors cause PNAG chain termination; consistent with the mechanism of PNAG polymerization from the non-reducing terminus. In vivo, expression of the GlcNAc-1-kinase NahK in E. coli provided a non-native GlcNAc salvage pathway that produced the UDP-GlcNAc analogue inhibitors in situ. The 6-fluoro and 6-deoxy derivatives were potent inhibitors of biofilm formation in the transformed strain, providing a tool to manipulate this key exopolysaccharide. Characterization of the UDP-GlcNAc pool and quantification of PNAG generation support PNAG termination as the primary in vivo mechanism of biofilm inhibition by 6-fluoro UDP-GlcNAc.

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