scholarly journals Green synthesis and Characterization of Silver Nanoparticles Synthesized Using Piper longum and its Antioxidant Activity

2021 ◽  
pp. 342-352
Author(s):  
Taanya Imtiaz ◽  
R. Priyadharshini ◽  
S. Rajeshkumar ◽  
Palati Sinduja
Keyword(s):  
Antioxidant Activity ◽  
Silver Nanoparticles ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Piper Longum ◽  
Pathological Conditions ◽  
Red Color ◽  
Anti Cancer ◽  
Anti Oxidant

Background: Piper longum, a traditional plant used for regenerative medicine commonly known as the long pepper, is used to treat many pathological conditions. Piper longum is used as a source for obtaining the production of various nanoparticles and testing their efficacy. Piper longum has many beneficial activities, such as antifungal, anti-amoebic, anti-asthmatic, anti-cancer, anti-oxidant, anti-inflammatory, antidepressants activities. Silver nanoparticles are widely and commonly used for its antimicrobial activity against various microorganisms. Our study aimed to synthesize Piper longum based silver nanoparticles and to investigate its anti-oxidant property. Materials and Methods: Piper longum plant extract was prepared and silver nanoparticles extract was synthesized. DPPH assay was then performed to assess the free radical scavenging activity of the Piper longum silver nanoparticle extract. The further analysis was that the formation of brownish-red color observed was evident, resulting in thesynthesization of silver nanoparticles. Results: Brown discolouration represents formation of silver nanoparticles and the antioxidant activity was noted to be from 60% to 70% from 10 to 50μL. But when compared to standard vitamin C values the Piper longum silver nanoparticles showed less antioxidant activity. Conclusion: The Piper longum mediated silver nanoparticles showed remarkable and considerable antioxidant activity but when compared with the standard sample values the antioxidant activity was low.

Free Radical Scavenging and Antioxidant Activity of Silver Nanoparticles Synthesized from Cuminum cyminum (Cumin) seed Extract

2021 ◽  
pp. 4349-4354
Author(s):  
Lubna Abdulazeem ◽  
Mohammad J. AL Jassani ◽  
Mustafa A. Al-Sheakh
Keyword(s):  
Antioxidant Activity ◽  
Silver Nanoparticles ◽  
Free Radical ◽  
Large Scale ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Seed Extract ◽  
Sem Images ◽  
Cumin Seed

Silver nanoparticles are extensive, applied in different fields. Green methods using plants have been used as renewable resources for the synthesis of biodegraded nanomaterials, thereby providing economic and safe synthesis routes. The green synthesis was done using the aqueous Cumin seed extract and as a bio-reducer agent and aqueous AgNO3 solution as a precursor under various conditions. The formation of silver nanoparticles was confirmed by the observation of the shift in color from colorless to dark brown. The synthesized AgNPs were characterized using UV/V is spectroscopy, XRD, FTIR, and SEM. Also, the synthesized AgNPs were evaluated for their antioxidant activity (In-vitro) by DPPH assay. The bio-reduced mixture showed a maximum peak at around 388nm. The XRD peaks were observed at 38o and 46o, corresponding to 111, 200, 220, and 311, and the peak widening suggested a smaller particle size. The FTIR absorption spectra indicated the presence of residual plant extract as a reducing agent in the reaction mixture. Also, analysis of C. cyminum seed extract strongly suggested the presence of OH stretching in alcoholic and phenolic compounds as the main phytochemicals parts, which is supported by a strong peak at approximately 3296cm−1. The SEM images clearly showed that AgNPs were almost spherical in shape and 48.7nm in size. The synthesized AgNPs showed almost the same pattern of ascorbic acid-free radical scavenging activity except at concentrations 100 and 50µg/ml with significant differences (P≤0.05) and it is dose-dependent. Silver nanoparticles can be synthesized on a large scale following a simple and eco-friendly method using C. cyminum seed extract that can be used as an effective antioxidant.

Phytochemical Screening and In Vitro Antioxidant Activity of Ethanolic Extract of Cassia occidentalis

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
S. Srividya ◽  
G. Sridevi ◽  
A. G. Manimegalai
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Phytochemical Analysis ◽  
Scavenging Activity ◽  
Cassia Occidentalis ◽  
Total Antioxidant ◽  
Anti Oxidant

The ethanolic extract of the leaves of Cassia occidentalis (Co) were subjected to phytochemical analysis by standard qualitative analysis and the invitro antioxidant activity was evaluated by determination of total antioxidant capacity, 1.1- diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, hydrogen peroxide (H2O2) radical scavenging activity, superoxide scavenging activity and Ferric reducing anti oxidant potential (FRAP). The analyses revealed that the ethanolic extract of Co was able to efficiently scavenge the free radicals in a dose dependant manner. The results were compared with the standard antioxidant ascorbic acid. The results have shown that crude ethanolic extract of the leaves of Co showed excellent antioxidant activity due to the presence of bioactive compounds namely alkaloids, betacyanin, cardiac glycosides, coumarins, flavonoids, phenols, steroids, saponins, tannins, terpenoids, anthraquinones and emodins.

scholarly journals Characterization of antioxidative peptide purified from black eelpout (Lycodes diapterus) hydrolysate

2019 ◽  
Vol 22 (1) ◽  
Author(s):  
Jung Kwon Lee ◽  
Hee-Guk Byun
Keyword(s):  
Antioxidant Activity ◽  
Amino Acid ◽  
Muscle Protein ◽  
Protein Hydrolysate ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Basic Research ◽  
Raw 264.7 Cells ◽  
Antioxidative Peptide

Abstract The functional peptides from protein hydrolysates of various fishery sources have been identified such as antioxidant activity. The main intention of this study was purification and characterization of antioxidative peptide from black eelpout muscle. The antioxidative peptides were purified from black eelpout (Lycodes diapterus) muscle using different proteases. Antioxidant activity of black eelpout hydrolysates was evaluated using DPPH radical scavenging activity. Among six hydrolysates, the pepsin hydrolysate had the highest antioxidant activity compared to the other hydrolysates. Therefore, it was further purified and a peptide with seven amino acid residues of DLVKVEA (784 Da) was identified by amino acid sequence analysis. The EC50 value for scavenging DPPH radicals by purified peptide was 688.77 μM. Additionally, the purified peptide exhibited protective effect against DNA damage induces by oxidation in mouse macrophages (RAW 264.7 cells). The results of this study suggest that black eelpout muscle protein hydrolysate could potentially contribute to development of bioactive peptides in basic research.

scholarly journals Preparation of Polyelectrolyte Complex Films of Chitosan-Alginate Incorporated by Eugenol and its Potency as an Antioxidant Packaging

2020 ◽  
Vol 9 (2) ◽  
pp. 88-95
Author(s):  
Baiq Amelia Riyandari
Keyword(s):  
Antioxidant Activity ◽  
Polyelectrolyte Complex ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Water Vapor Permeability ◽  
Vapor Permeability ◽  
Elongation At Break ◽  
Carboxylate Groups ◽  
Good Percentage

Preparation of PEC chitosan-alginate films incorporated by eugenol has been investigated. Incorporation of eugenol in chitosan-alginate films was conducted by using the different concentration of eugenol including 0.25% 0.5%, and 1% (% w/v). The effect of eugenol incorporation in chitosan-alginate films was investigated through some properties of the films such as tensile strength, elongation at break, transparency value, and water vapor permeability. Meanwhile, the effectiveness of eugenol incorporation as an active compound of the films was investigated from antioxidant activity of chitosan-alginate films incorporated eugenol. Polyelectrolyte complex (PEC) films of chitosan-alginate was occurred through molecular interaction between polycationic groups of chitosan and polyanionic groups of alginate. The formation of chitosan-alginate PEC films was synthesized at pH ± 4.0. Based on FTIR analysis, the ionic interaction between amine groups (–NH3+) and carboxylate groups (–COO¬) formed strongly. Characterization of films also indicated that PEC films of chitosan-alginate incorporated of eugenol was formed. Study showed that PEC chitosan-alginate films had good mechanical properties. Antioxidant activity assay through  fixed reaction time method  using DPPH radical (α,α-difenil-β- pikrilhidrazil) resulted in good percentage of radical scavenging activity (%RSA) from the films. The E3 films which contain 1% eugenol has 55.99% of  RSA value in 96 hours.

scholarly journals Synthesis and Characterization of Vanadium (IV)-Flavonoid Complexes and its Antioxidant ability toward Superoxide and Radical Scavenging

2020 ◽  
Author(s):  
Himyan Akbar ◽  
Salma Habib ◽  
Mohammed Mahroof Tahir ◽  
Lakshmaiah Sreerama
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Synthesis And Characterization ◽  
Vanadium Complex ◽  
Antioxidant Ability ◽  
Ic50 Value ◽  
Anti Oxidant ◽  
Metal Charge Transfer

In this project Vanadium complex -Vanadium (IV) - flavone was synthesized using vanadium (IV) acetylacetonate (VO(acac)2) complex and 3-hydroxy-6-methyl flavone ligand. The complex stability was checked using FTIR and UV-vis spectroscopies. Peackes around 990 cm-1 conforms the formation of (V=O) in the complex, as well as (V-O) around 790 cm-1. In UV-Vis spectrum peak around 400-450 nm was noticed, which conforms the formation of the vanadium complex that correspond to the ligand to metal charge transfer (LMCT) transition. The radical scavenging abilities of vanadium complex were investigated using DPPH. The anti-oxidant activity using (BHA) as a standard reference, the complex synthesized displayed strong DPPH antioxidant radical scavenging activity compared to VO(acac)2 and BHA, with IC50 value of (105, 95 and 96) mM respectively. The absorbance in which the reducing power occurred were found to be (0.397, 0.825 and 0.228) for the complex, VO(acac)2 and BHA.

Design, Synthesis and Characterization of Some Novel 3-Coumarinyl- 5-aryliden-1,3-thiazolidine-2,4-diones and Their Antioxidant Activity

2011 ◽  
Vol 66 (2) ◽  
pp. 177-183 ◽  
Author(s):  
Milan Čačić ◽  
Maja Molnar
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Aromatic Aldehydes ◽  
Free Radical Scavenging ◽  
Biologically Active ◽  
Synthesis And Characterization ◽  
Knoevenagel Reaction ◽  
Design Synthesis

In our effort to obtain biologically active compounds, new 3,5-disubstituted 1,3-thiazolidine-2,4- diones (5a - r) were synthesized. A series of 5-arylmethylidene-1,3-thiazolidine-2,4-diones (3a - r) were prepared by Knoevenagel reaction from 1,3-thiazolidine-2,4-dione (2) and appropriate aromatic aldehydes. Condensation of 3a - r with 7-hydroxy-4-bromomethyl-2-oxo-2H-chromene (1) afforded novel 3-(7-hydroxy-2-oxo-2H-chromen-4-ylmethyl)-5-arylidene-1,3-thiazolidine-2,4-diones 5a - r. Compounds 3a - r and 5a - r were evaluated for their antioxidant activity (DPPH free radical scavenging activity).

scholarly journals In Vitro Antioxidant Activity of Hydrophila auriculata Leaves Extract and Silver Nanoparticles

2018 ◽  
Vol 4 (5) ◽  
pp. 549-551 ◽  
Author(s):  
M. Ravishankar ◽  
V. Christibai Juliet Esther
Keyword(s):  
Antioxidant Activity ◽  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Elemental Silver ◽  
Total Antioxidant ◽  
Dose Dependent ◽  
In Vitro Antioxidant Activity

Antioxidant activity of Hydrophila auriculata leaf extract and silver nanoparticles (AgNPs) were carried out for proving its utility in free radical mediated diseases. The flower extract and silver nanoparticles were screened for in vitro antioxidant activity by oxygen radical scavenging such as DPPH, total antioxidant assay and superoxide radical scavenging activity at different concentrations. The antioxidant potential was found to be dose dependent. AgNPs possess probable antioxidant activity as compared with Hydrophila auriculata leaf extract and near to the standard. It is an obvious indication that the resulting activity of the AgNPs is not due to any capping agents, which are found in minute amounts but is due to the elemental silver.

Preliminary Phytochemical Screening, In Vitro Antioxidant Activity, Topical and Oral Formulation of Extract of Woodfordia fructicosa and Gardenia gummifera

2017 ◽  
Vol 8 ◽  
pp. 16-26
Author(s):  
Hemant K. Nagar ◽  
Mahendra S. Ranawat
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Oral Formulation ◽  
Dpph Method ◽  
Anti Oxidant ◽  
Sedimentation Volume ◽  
Dpph Radical Scavenging

Woodfordia fructicosa and Gardenia gummifera are traditionally claimed to be useful in treatment of number of diseases. The main aim of this study was to evaluate preliminary phytochemical tests and antioxidant activity of ethanolic extract of Woodfordia fructicosa flowers and Gardenia gummifera leaves and finally extracts were formulated in Gels and Suspensions. In vitro antioxidant activity was evaluated by DPPH method. The different concentration (100, 200, 300, 400 and 500 μg/ml) of standard and test samples were prepared. The anti-oxidant activity is exhibited in percentage inhibition. The DPPH radical scavenging activity of both the extracts increases with increasing concentration. The prepared gels were evaluated physical appearance, homogeneity, Grittiness, Spreadability and Viscosity. The prepared suspensions were evaluated organoleptic properties, pH and Sedimentation Volume.

scholarly journals Evaluation of Antioxidant Activity of Synthesized Silver Nanoparticles from Citrus Aurantium Peels Extract by Using the Green Method

2021 ◽  
Vol 16 (2) ◽  
Author(s):  
Sumit Ringwal ◽  
Ankit Singh Bartwal ◽  
S C Nautiyal ◽  
Satish Chandra Sati
Keyword(s):  
Antioxidant Activity ◽  
Silver Nanoparticles ◽  
Radical Scavenging Activity ◽  
Research Work ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Citrus Aurantium ◽  
Research Article ◽  
Ic50 Value

The substance which avoids oxidant of easily oxidizing substance even at low concentration is called antioxidant when antioxidant reacts with reactive oxygen species (ROS) or reactive nitrogen species (RNS) they often convert themself into antioxidant radical. Although such a radical has a reduced ability to react with imperative cellular targets, it can still cause damage. To decrease reactivity and reduction potential further down, the ‘antioxidant radical' react with another antioxidant and this reaction continues in a stepwise manner until antioxidant radical’ is no longer damage the cells like lipids, protein, DNA, and other important cellular molecules. In this research article, we had scanned our previously synthesized silver nanoparticles from Citrus aurantium peels extracts; Synthesis and characterization part of the research work is already in communication we are extending our research work on previously synthesized silver nanoparticles. In this research article, we had scanned the antioxidant activity of synthesized AgNPs was determined by H2O2 free radical scavenging activity, we obtained antioxidant activity of BHT with IC50 Value 65.52 µg/mL and antioxidant activity of synthesized AgNPs with IC50 Value 93.92 µg/mL. Antioxidant activity of synthesized AgNPs was also determined by DPPH free radical scavenging activity. We obtained antioxidant activity of BHT with IC50 Value 65.99 µg/mL and antioxidant activity of synthesized AgNPs with IC50 Value 99.68 µg/mL.

Evaluation of antioxidant and Anthelmintic activity of methanolic flower extract of Nyctanthes arbor-tristis

2018 ◽  
Vol 9 (1) ◽  
pp. 78
Author(s):  
Vijaya Jyothi M ◽  
Bhargav E ◽  
Pavan Kumar K ◽  
Praneeth Gowd K ◽  
Ram Pavan S
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Iridoid Glycosides ◽  
Radical Scavenging ◽  
Anthelmintic Activity ◽  
Total Phenolic ◽  
Standard Drug ◽  
Flower Extract ◽  
Reducing Ability ◽  
Total Flavonoids Content

Nyctanthes arbour-tristis is a shrub belongs to the family Oleaceae. The flowers of this plant are fragrant since the presence of flavonol glycosides. It has also been reported for the presence of β-sitosterol, iridoid glycosides, tannins etc., and known to have immunostimulant, hepatoprotective, antiviral and antifungal activities. In the present study an attempt is made to identify antioxidant capacity and anthelminthic potential of methanolic flower extract of Nyctanthes arbour-tristis. Antioxidant activity was evaluated by total phenolic content assay, total flavonoids content assay, free radical scavenging activity and reducing ability methods. Anthelmintic activity was evaluated on Perithima posthuma using Piperazine citrate as standard drug. The results obtained for the above activities reveals that Nyctanthes arbour-tristis shows considerable antioxidant activity for all the methods and anthelminthic potential at 300 mg/ml. Keywords: arbour-tristis; antioxidant activity; anthelminthic activity; Perithima posthuma; Piperazine citrate.

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