In vitro evaluation of cytotoxicity, possible alteration of apoptotic regulatory proteins, and antibacterial activity of synthesized copper oxide nanoparticles

Background: Recently, nanotechnology has been demonstrated to be a promising application to overcome the problem of antibiotic resistance. In the present study, we aimed to determine the antibacterial activity of copper oxide nanoparticles (CuO NPs) on several multiple-drug resistant (MDR) uropathogenic strains. Methods: This in vitro case-control study was performed on 4 uropathogenic bacteria including Staphylococcus aureus, Enterococcus faecalis, Escherichia coli and Pseudomonas aeruginosa. The antibacterial property was evaluated by well diffusion method at different concentrations of CuO NPs. Results: Overall, NPs concentration of 10, 25 and 50 µg/mL showed the remarkable antibacterial activity. A lower effect was seen against S. aureus strains. CuO NPs exhibited maximum bacterial growth inhibition against E. faecalis strains. In most of the cases, the zone of inhibition in 50 µg/mL concentration was closest to control positive antibiotics. Conclusion: In summary, CuO NPs as an alternative to conventional antibiotics that are currently used showed dose-dependent on antibacterial activity against different uropathogens, specificity towards pathogenic Gram-positive bacteria. This promising antibacterial activity of CuO NPs suggesting the development of NPs coatings on the different surface of biomedical materials for applications in different antimicrobial control systems.

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ANTIBIOTIC PROFILE AND ANTIBACTERIAL ACTIVITY OF COPPER OXIDE NANOPARTICLES AGAINST Pseudomonas fluorescens ISOLATED FROM WOUND INFECTION

International Journal of Drug Delivery Technology ◽

10.25258/ijddt.v9i3.10 ◽

2019 ◽

Vol 9 (o3) ◽

Author(s):

Haider Qassim Raheem ◽

Takwa S. Al-meamar ◽

Anas M. Almamoori

Keyword(s):

Antibacterial Activity ◽

Copper Oxide ◽

Pseudomonas Fluorescens ◽

Wide Spectrum ◽

Copper Oxide Nanoparticles ◽

Morphological Properties ◽

Clinical Samples ◽

Oxide Nanoparticles ◽

Cuo Nps ◽

Disk Diffusion Assay

Fifty specimens were collected from wound patients who visited Al-Hilla Teaching Hospital. The samples were grown on Blood and MacConkey agar for 24-48 hr at 37oC. The bacterial isolates which achieved as a pure and predominant growth from clinical samples as Pseudomonas fluorescens, were identified using morphological properties and Vitek2 system. The anti-bacterial activity of copper oxide nanoparticles (CuO NPs) against was tested by (disk diffusion assay) using dilutions of (400, 200, 100, 50, 25, and 12.5‎µ‎g/ml). The (MIC and MBC) of each isolate was determined. CuO NPs shows wide spectrum antibacterial activity against tested bacteria with rise zone of inhibition diameter that is proportionate with the increase in nanoparticle concentration. The MIC of CuO NPs extended from 100-200‎µ‎g/ml and the MBC ranged from 200-400‎µ‎g/ml. The antibiotic profile was determined by Viteck 2 compact system (Biomérieux). CuO NPs‎ found highly effective and safe in P. fluorescens wounds infections comparing with used antibiotics.

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In vitro improvement and rooting of banana plantlets using antifungal nanocomposite based on myco-synthesized copper oxide nanoparticles and starch

Biomass Conversion and Biorefinery ◽

10.1007/s13399-021-01784-4 ◽

2021 ◽

Author(s):

Mohamed Hasanin ◽

Amr Hosny Hashem ◽

Islam Lashin ◽

Sayed A. M. Hassan

Keyword(s):

Copper Oxide ◽

Copper Oxide Nanoparticles ◽

Oxide Nanoparticles

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Green synthesis of copper oxide nanoparticles using Cedrus deodara aqueous extract for antibacterial activity

Materials Today Proceedings ◽

10.1016/j.matpr.2020.05.472 ◽

2020 ◽

Author(s):

M. Ramzan ◽

Raphael.M. Obodo ◽

S. Mukhtar ◽

S.Z. Ilyas ◽

Fahad Aziz ◽

...

Keyword(s):

Antibacterial Activity ◽

Copper Oxide ◽

Green Synthesis ◽

Aqueous Extract ◽

Copper Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Cedrus Deodara

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Green Synthesis, Characterization and Antibacterial Activity of Copper Oxide Nanoparticles Using Annona muricata Stem Extract

International Journal for Research in Applied Science and Engineering Technology ◽

10.22214/ijraset.2021.39498 ◽

2021 ◽

Vol 9 (12) ◽

pp. 1205-1214

Author(s):

Shivani Kushwaha

Keyword(s):

Antibacterial Activity ◽

Copper Oxide ◽

Copper Sulphate ◽

Copper Oxide Nanoparticles ◽

Maximum Activity ◽

Cuo Nanoparticles ◽

Oxide Nanoparticles ◽

Gradual Change ◽

Annona Muricata ◽

Stem Extract

Abstract: Nanotechnology is a rising field of science and technology that deals with the particles having size in the range of 1 to 100 nm. Copper oxide nanoparticles has many properties like antifungal activity, antibacterial activity, optical properties, conductive properties, etc. Due to its demand of diversified use, copper oxide nanoparticles were fabricated using ecofriendly and non-toxic Annona muricata stem extract. The extract with copper sulphate pentahydrate showed gradual change in the colour of the extract from brown to green which indicates the CuO nanoparticles synthesis. The fabrication is followed by characterization of CuO nanoparticles using UV-vis spectroscope, FTIR, XRD and SEM. The characterization showed roughly spherical shaped nanoparticles in the range of 100nm with high crystalline monoclinic phase. FTIR absorption spectra conclude that the compounds attached with copper oxide nanoparticles could be polyphenols with aromatic ring. The CuO nanoparticles exhibited antibacterial activity; it showed the maximum activity against E.coli (18 mm). Keywords: Annona muricata, copper sulphate pentahydrate, FTIR, nanomaterials, SEM, XRD.

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